Study on the novel bioactive substances in follicular fluid regulating a oocyte maturation and its development.

1. 为了评价卵泡发育，用放射免疫法测定了18例卵巢过度刺激妇女的血清抑制素。早期卵泡期血清抑制素与给药当日卵泡直径<大于等于17mm (r=0.57)，卵泡直径<大于等于14mm （r=0.67）相关。这些数据提示：在卵泡早期，血清抑制素可能是预测随后卵泡生长的有效指标；在排卵前阶段，血清抑制素可能是hyper2期间卵泡发育的适用指标之一。分析了激活素A对经PMSG处理的未成熟大鼠卵母细胞减数分裂成熟的影响；激活素A被纯化为红细胞分化因子，不仅加速了卵泡封闭卵母细胞和卵丘复合物的成熟，而且加速了脱落卵母细胞的成熟，这是通过增加卵母细胞生发囊泡破裂（GVBD）的百分比来测量的。激活素A在GVBD抑制剂如环磷酸腺苷（cyclic-AMP）存在的情况下不加速卵母细胞成熟，表明激活素A是一种有效的体外卵母细胞成熟刺激剂。在1-3天的时间内，每天sc注射40 ug激活素A，使未成熟雌性大鼠血清FSH、抑制素、雌二醇、子宫重量和卵巢FSH受体显著增加。每天sc注射5 ug或20 ug激活素a，连续6天，卵巢重量明显增加，卵巢卵泡发育大。与同时注射激活素- a和5IU PMSG相比，在垂体切除的未成熟雌性大鼠中，同时注射激活素- a和5IU PMSG诱导卵巢和子宫重量，血清抑制素和雌二醇水平显著增加。

1. In order to appraise follicular development, human serum inhibin was measured daily by radioimmunoassay in 18 women undergoing ovarian hyperstimulation. Serum inhibin in the early follicula phase correlated with the number of follicles<greater than or equal> 17 mm in diameter(r=0.57), follicles<greater than or equal>14 mm in diameter(r=0.67)on the day of hCG administration. These deta suggest that : In early follicular phase, serum inhibin may be a valid index to predict ensuing follicular growth ; In the preovulatory Phase, serum inhibin may by one of applicable indexes of follicular development during the hyper2. The effect of activin A on meiotic maturation was analyzed in oocyte from imroature rats treated with PMSG ; Activin A, which was purified as the erythroid differentiation factor, accelerated the maturation of not only follicle-enclosed oocytes and oocytecumulus complexes, but also denuded oocytes, as measured by an increase in the percentage of oocytes with germinal vesicle breakdown(GVBD). Oocyte maturation was not accelerated by activin A in the presence of the inhibitor of GVBD such as cyclic-AMP, These results showed activin A is a potent in vitro stimulator of oocyte maturation.3. Daily sc injections of 40 ug activin A over a period of 1-3 days to intact immature female rats caused a significant increase in serum FSH, inhibin, estradiol, uterine weight and ovarian FSH receptors. Daily sc injections of 5 ug or 20 ug activin-A for 6days caused a marked increase in ovarian weight and the development of large ovarian follicles. Simultaneous injections of both activin-A and 5IU PMSG induced a significant increase in ovarian and uterine weight, serum inhibin and e. stradiol levels, compared to simultaneous injections of both vehicle and PMSG in the hypophysectomized immature female rats.