Study on the novel bioactive substances in follicular fluid regulating a oocyte maturation and its development.

1.为评价卵泡发育，采用放射免疫法测定了18例卵巢超排卵妇女的血清抑制素水平。卵泡早期血清抑制素水平与注射hCG当天直径大于或等于17 mm的卵泡数(r=0.57)、直径大于或等于14 mm的卵泡数(r=0.67)呈正相关。提示：在卵泡早期，血清抑制素可能是预测后续卵泡生长的有效指标；在排卵前期，血清抑制素可能是卵泡发育的实用指标之一。分析了激活素A对PMSG处理大鼠卵母细胞减数分裂成熟的影响，纯化的红系分化因子激活素A不仅促进了卵泡包裹的卵母细胞和卵丘复合体的成熟，而且还促进了裸卵母细胞的成熟，其结果是生发泡破裂(GVBD)的卵母细胞比例增加。激活素A在GVBD抑制剂如环磷酸腺苷的存在下不能促进卵母细胞的成熟，说明激活素A是一种有效的卵母细胞体外成熟刺激剂。未成熟雌性大鼠每日皮下注射激活素A 40微克，连续1~3天，血清FSH、抑制素、雌二醇、子宫重量和卵巢FSH受体均显著增加。每日皮下注射激活素-A 5ug或20ug，连续6天，可引起卵巢重量显著增加和大卵泡发育。与同时注射赋形剂和PMSG相比，同时注射激活素-A和5IU PMSG可显著增加去垂体未成熟雌性大鼠的卵巢和子宫重量、血清抑制素和雌二醇水平。

1. In order to appraise follicular development, human serum inhibin was measured daily by radioimmunoassay in 18 women undergoing ovarian hyperstimulation. Serum inhibin in the early follicula phase correlated with the number of follicles<greater than or equal> 17 mm in diameter(r=0.57), follicles<greater than or equal>14 mm in diameter(r=0.67)on the day of hCG administration. These deta suggest that : In early follicular phase, serum inhibin may be a valid index to predict ensuing follicular growth ; In the preovulatory Phase, serum inhibin may by one of applicable indexes of follicular development during the hyper2. The effect of activin A on meiotic maturation was analyzed in oocyte from imroature rats treated with PMSG ; Activin A, which was purified as the erythroid differentiation factor, accelerated the maturation of not only follicle-enclosed oocytes and oocytecumulus complexes, but also denuded oocytes, as measured by an increase in the percentage of oocytes with germinal vesicle breakdown(GVBD). Oocyte maturation was not accelerated by activin A in the presence of the inhibitor of GVBD such as cyclic-AMP, These results showed activin A is a potent in vitro stimulator of oocyte maturation.3. Daily sc injections of 40 ug activin A over a period of 1-3 days to intact immature female rats caused a significant increase in serum FSH, inhibin, estradiol, uterine weight and ovarian FSH receptors. Daily sc injections of 5 ug or 20 ug activin-A for 6days caused a marked increase in ovarian weight and the development of large ovarian follicles. Simultaneous injections of both activin-A and 5IU PMSG induced a significant increase in ovarian and uterine weight, serum inhibin and e. stradiol levels, compared to simultaneous injections of both vehicle and PMSG in the hypophysectomized immature female rats.