A study on cardiac injury of methamphetamine abusers

甲基苯丙胺滥用者心脏损伤的研究

• 批准号: 61570290
• 负责人: MATOBA Ryoji
• 金额: $ 1.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570290/
• 关键词: methamphetamine; cardiac injury; catecholamine; B-blocker; Ca-antagonist; Ca-antagonisr; 心筋症

To investigate the effects of long-term mechamphetaminc(MA) administration in animals. MA 5. 10. 20 mg/kg b.w. was injected to ddy male mice 6 times per week for 2 or 4 weeks. In microscopic observations there were the findings: hypertrophy. atrophy. eosinophilic changes, vacuolation contraction band nccrosis and disarray of myofibers. minimum patchy fibrosis. interstitial edema and infiltrations of small round cells. These lesions were mainly seen at the left ventricle and the septum except disarray of myotibers which was prominent at the triangle region. Electorn microscopy revealed further appearance of irregularity of sarcolomma, hypercontraction of sarcomere, thickning of % band. increase in myofibrillar elements and mitochondria and dilatation of the sarcomplasmic reticuliums. Dissapearance of myofibrils. degenerations of mitochondia and myofibrillar disarray were sometimes seen. Furthermore. many single membrane vesicles were sometimes observed bancath the sarcolemma. Thesed sur … More face vesiclees were similar to dilated sarcoplasmic reticulums in size, sometimes fused with sarcolomma and seen in the basement membrane. inthe cndothelial cells and in the capillary lumen. In general, the severity of these cardiac lesions were in proportion to both the doso and the duration of MA administrated. but disarray of myofibers were rather found frequently in the group administered smaller dose of MA. We then compared the lesions by MA with those after administration of catecholamine. and also examined the effect of -blocker and Ca-antagonist. The cardiac lesions after norepincphrine administration were similar to those after MA administration but to a limited extent. On the contrally. premedication of propranolol and verapamil prevented the appcarance of the cardiac lesion. isolorm pattern of cardiac myosin of the mice administered MA as by pyrophosphate gei cicotrophoresis showed to so remarkable difference from that of controll mice. Thus. various cardiac lesions found in MA abusers seemed to be explained by the cardiac toxicity of MA by itself. Furtheermore. our result suggests that. MA induces cardiac injury through catecholamine released from nerve endings. and possibly caleium everioad may be also involved. Less

目的探讨长期给予动物甲苯丙胺（MA）的影响。 MA 5. 10. 20 毫克/千克体重每周向 ddy 雄性小鼠注射 6 次，持续 2 或 4 周。显微镜观察发现：肥大。萎缩。嗜酸性粒细胞变化、空泡收缩带坏死和肌纤维紊乱。最小的斑片状纤维化。间质水肿和小圆形细胞浸润。这些病变主要见于左心室和室间隔，但三角区肌纤维排列紊乱除外。电子显微镜进一步显示肌节不规则、肌节过度收缩、%带增厚。肌原纤维成分和线粒体增加以及肌浆网扩张。肌原纤维消失。有时可见线粒体变性和肌原纤维紊乱。此外。有时在肌膜处观察到许多单膜囊泡。面部的这些囊泡大小与扩张的肌浆网相似，有时与肌膜融合，可见于基底膜。在内皮细胞和毛细血管腔中。一般来说，这些心脏病变的严重程度与MA的剂量和持续时间成正比。但在给予较小剂量MA的组中，经常发现肌纤维紊乱。然后我们将 MA 造成的病变与给予儿茶酚胺后的病变进行比较。并检查了β-阻滞剂和Ca-拮抗剂的作用。去甲肾上腺素给药后的心脏病变与MA给药后相似，但程度有限。相反。术前使用普萘洛尔和维拉帕米预防了心脏病变的出现。通过焦磷酸盐凝胶电泳施用MA的小鼠的心肌肌球蛋白异构体模式显示出与对照小鼠的显着差异。因此。 MA 滥用者中发现的各种心脏病变似乎可以用 MA 本身的心脏毒性来解释。此外。我们的结果表明这一点。 MA通过神经末梢释放的儿茶酚胺诱发心脏损伤。可能还涉及大量钙。较少的

项目成果

的場梁次: 大阪大学医学雑誌. 40(4). 341-353 (1988)

间场良治：大阪大学医学杂志 40(4)（1988）。

Ryoji Matoba.: "A Morphological Study on Mice Hearts Following after Long-term Methamphetamine Administration" Medical Journal of Osaka Unviersiyt Japanese Edition. 40. 341-353 (1988)

Ryoji Matoba.：“长期服用甲基苯丙胺后小鼠心脏的形态学研究”大阪大学医学杂志日文版。

的場梁次: 大阪大学医学雑誌. (1988)

场良二：大阪大学医学杂志（1988）。