Expression of Bcl-2 and Bax mRNA during ischemia-reperfusion in isolated rat cardiomyocytes

我们的研究目的是获得可靠的参数作为早期急性心肌梗死法医尸检病例的诊断标志。采用缺氧/再氧化心肌细胞模型，采用定量RT-PCR方法检测Bax/Bcl-2 mRNA比值。缺氧12小时组和缺氧24小时组Bax/Bcl-2 mRNA比值均升高。此外，琼脂糖凝胶电泳检测心肌细胞的DNA片段。另一方面，复氧组心肌细胞Bax/Bcl-2 mRNA比值在复氧3小时后下降。上述结果提示，作为细胞凋亡参数的Bax/Bcl-2 mRNA比值，可作为法医尸检病例早期急性心肌梗死诊断指标的可靠参数。然而，由于死后变化的影响，凋亡细胞的检测比较困难。

The purpose of our study was to achieve reliable parameter as a diagnostic marker in an early stage of acute myocardial infarction to forensic autopsy cases. The Bax/Bcl-2 mRNA ratio was examined by quantitative RT-PCR methods using a cardiomyocyte model of hypoxia/reoxygenation. There was a increase in Bax/Bcl-2 mRNA ratio in both groups of 12-hour hypoxia and 24-hour hypoxia. Moreover, DNA fragmentation of cardiomyocytes was also detected by agarose gel electrophoresis. On the other hand, there was a decrease in Bax/Bcl-2 mRNA ratio in cardiomyocytes of reoxygenation groups which were exposed to reoxygenation for 3 hours. These results suggest that Bax/Bcl-2 mRNA ratio, which is a parameter of apoptosis, may be a reliable parameter as a diagnostic marker in an early stage of acute myocardial infarction to forensic autopsy cases. However, it would be difficult to detect apoptotic cells owing to the effects of the postmortem change.