Expression of Bcl-2 and Bax mRNA during ischemia-reperfusion in isolated rat cardiomyocytes

大鼠离体心肌细胞缺血再灌注过程中Bcl-2和Bax mRNA的表达

• 批准号: 14370154
• 负责人: MATOBA Ryoji
• 金额: $ 4.48万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370154/
• 关键词: diagnostic marker; quantitative RT-PCR; apoptosis; ischemia-reperfusion; Bcl-2; Bax; 心筋梗塞; 虚血誘発性アポトーシス; 培養心筋細胞; アポトーシス関連遺伝子

The purpose of our study was to achieve reliable parameter as a diagnostic marker in an early stage of acute myocardial infarction to forensic autopsy cases. The Bax/Bcl-2 mRNA ratio was examined by quantitative RT-PCR methods using a cardiomyocyte model of hypoxia/reoxygenation. There was a increase in Bax/Bcl-2 mRNA ratio in both groups of 12-hour hypoxia and 24-hour hypoxia. Moreover, DNA fragmentation of cardiomyocytes was also detected by agarose gel electrophoresis. On the other hand, there was a decrease in Bax/Bcl-2 mRNA ratio in cardiomyocytes of reoxygenation groups which were exposed to reoxygenation for 3 hours. These results suggest that Bax/Bcl-2 mRNA ratio, which is a parameter of apoptosis, may be a reliable parameter as a diagnostic marker in an early stage of acute myocardial infarction to forensic autopsy cases. However, it would be difficult to detect apoptotic cells owing to the effects of the postmortem change.

本研究的目的是获得可靠的参数作为急性心肌梗死早期法医尸检的诊断标记物。采用心肌细胞缺氧/复氧模型，用定量RT-PCR法检测Bax/Bcl2mRNA的表达。低氧12小时组和低氧24小时组Bax/Bc l-2 m RNA比值均升高。琼脂糖凝胶电泳法检测心肌细胞DNA片段化。复氧3h后，复氧组心肌细胞Bax/Bcl2mRNA比值下降。提示Bax/Bcl2mRNA比值作为细胞凋亡的一个参数，在急性心肌梗死早期和法医尸检病例中可能是一个可靠的诊断指标。然而，由于死后变化的影响，很难检测到凋亡细胞。

项目成果

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