Expression of Bcl-2 and Bax mRNA during ischemia-reperfusion in isolated rat cardiomyocytes

本研究的目的是为法医尸检急性心肌梗死的早期诊断提供可靠的指标。采用定量RT-PCR方法检测心肌细胞缺氧/复氧模型中Bax/Bcl-2 mRNA的比值。缺氧12 h和24 h组Bax/Bcl-2 mRNA比值均升高。琼脂糖凝胶电泳检测心肌细胞DNA断裂。再给氧3 h后，心肌细胞Bax/Bcl-2 mRNA比值下降。提示Bax/Bcl-2 mRNA比值作为细胞凋亡的指标，可作为急性心肌梗死早期诊断的可靠指标。然而，由于死后变化的影响，很难检测到凋亡细胞。

The purpose of our study was to achieve reliable parameter as a diagnostic marker in an early stage of acute myocardial infarction to forensic autopsy cases. The Bax/Bcl-2 mRNA ratio was examined by quantitative RT-PCR methods using a cardiomyocyte model of hypoxia/reoxygenation. There was a increase in Bax/Bcl-2 mRNA ratio in both groups of 12-hour hypoxia and 24-hour hypoxia. Moreover, DNA fragmentation of cardiomyocytes was also detected by agarose gel electrophoresis. On the other hand, there was a decrease in Bax/Bcl-2 mRNA ratio in cardiomyocytes of reoxygenation groups which were exposed to reoxygenation for 3 hours. These results suggest that Bax/Bcl-2 mRNA ratio, which is a parameter of apoptosis, may be a reliable parameter as a diagnostic marker in an early stage of acute myocardial infarction to forensic autopsy cases. However, it would be difficult to detect apoptotic cells owing to the effects of the postmortem change.