Simultaneous detection of miltiple STR loci

采用荧光技术同时扩增3个STRs基因座（VWA、F13AO1和FES）。等位基因频率为：VWA(n=114) 14: 0.110；15:0 .035；16:0 .215；17: 0.250；18: 0.250；19: 0.118；20:0.022， FES(n=103) 9:0.015；10:10 .087；11: 0.364；12:0 .320；13:0 .209；14: 0.005, F13A01 (n=114) 2.3: 0.356；4: 0.081；5:0 .021；6:5 . 42。3个位点均观察到Hardy-Weinberg平衡。最近证明了X和Y染色体STR位点的法医用途。1个四联PCR，使用2个X-和2个y -STR (STRX1/HPRTB和DYS39O/DYS393)， 2个双联PCR，每个使用和X-和一个STR (ARA/DYS39O和ARA/DYS393)， PCR产物检测使用自动DNA测序仪。这种方法使我们不仅可以确定样本供体的性别，还可以确定样本的X和/或y-STR基因型。雄性生物标本在半双链pcr中扩增出4个扩增产物，在双链pcr中扩增出2个扩增片段，而雌性生物标本在四双链pcr中只扩增出2个X-STR片段，在双链pcr中也只扩增出1个X-STR片段。因此，我们的研究为法医实践中的许多活动提供了有用的信息，例如身份测试，亲子鉴定，特别是缺陷病例，人口数据的汇编，以及从单个PCR中确定生物样本的性别。

A protoco1 for simultanecus amplification of three STRs loci (VWA, F13AO1 and FES) detected by a fluorescence based technology was performed. Allele frequencies were as follws : VWA(n=114) 14 : 0.110 ; 15 : 0.035 ; 16 : 0.215 ; 17 : 0.250 ; 18 : 0.250 ; 19 : 0.118 ; 20 : 0.022, FES(n=103) 9 : 0.015 ; 10 : 0.087 ; 11 : 0.364 ; 12 : 0.320 ; 13 : 0.209 ; 14 : 0.005, F13A01 (n=114) 2.3 : 0.356 ; 4 : 0.081 ; 5 : 0.021 ; 6 : 0.542. Hardy-Weinberg equilibrium was observed for three loci.The forensic usefulness of X and Y chromosomal STR loci has recently been demonstrated. One quadruplex-PCR, using 2 X- and 2 Y-STRs (STRX1/HPRTB and DYS39O/DYS393), and 2 duplex-PCRs, each using and X- and a STR (ARA/DYS39O and ARA/DYS393), and detection of PCR prcducts was performed by using an automated DNA sequencer. This approach allows us to determine not only the sex of the donor of a sample, but also the X- and/or y-STR genotypes of the sample. A male biological specimen yields 4 amplified products in quayadruplex-PCR and 2 amplified fragments in duplex-PCRs, whereas a female biological specimen yields only 2 amplified fragments of X-STR in quadruplex-PCR and one fragment, also of X-STR, in duplex-PCRs. Our study thus provides useful information for many activities in forensic practice, such as identity testing, paternity testing, especially of deficiency cases, compilation of popilation data, and sex determination of a biological sample from a single PCR.