Detection of cardiomyocyte apoptosis in acute myocardial infarction

急性心肌梗死心肌细胞凋亡的检测

• 批准号: 10670397
• 负责人: MATOBA Ryoji
• 金额: $ 2.24万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670397/
• 关键词: myocardial infarction; diagnostic marker; apoptosis; 早期診断マ-カ-; アポト-シス

The purpose of our study was to achieve reliable parameter and apply apoptotic cells as a diagnostic marker in an early stage of acute myocardial infarction to forensic autopsy cases. Myocardial tissue taken from forensic autopsy cases were examined by immunohistochemical and molecular-biological methods using the terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) and the DNA laddering method. In cases of acute myocardial infarction within 24 hours postmortem time, the nuclei of cardiomyocytes were stained strongly positive with TUNEL method and DNA fragmentation of myocardial cells was detected by agarose gel electrophoresis. In other cases such as asphyxia, tetrodotoxin poisoning, alcohol poisoning, head injury, and myocarditis, no apoptotic cells were found. However, it was difficult to discriminate between apoptotic cells and TUNEL-positive cells owing to the effects of the postmortem change.

本研究的目的是获得可靠的参数，并将凋亡细胞作为急性心肌梗死早期诊断标志物应用于法医尸检病例。应用脱氧核苷酸末端转移酶介导的dUTP生物素缺口末端标记法（TUNEL）和DNA梯状条带法，对法医尸检心肌组织进行了免疫组化和分子生物学检测。死后24小时内的急性心肌梗死病例，心肌细胞核TUNEL法染色呈强阳性，琼脂糖凝胶电泳检测心肌细胞DNA断裂。在窒息、河豚毒素中毒、酒精中毒、颅脑损伤、心肌炎等病例中，未发现凋亡细胞，但由于死后变化的影响，很难区分凋亡细胞和TUNEL阳性细胞。

项目成果

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