Effects of anoxia or hypoxia on morphological changes and leakage of cytoplasmic substances in cultured adult rat cardiomyocytes.

为了建立早期缺血性心脏病的诊断，我们利用培养的成年大鼠心肌细胞，研究了在体外缺氧(或缺氧)过程中是否导致了包括细胞死亡在内的细胞损伤。在20 mm HgPO2低氧条件下，细胞收缩、肿胀、横纹消失、滤过泡形成等形态改变持续4h。细胞外培养上清液中肌酸磷酸激酶的含量也增加。此外，较长的低氧孵育时间会导致DNA降解，包括180bp的DNA片段。这些结果提示，法医病理资料中的这些形态变化和细胞凋亡可能有助于缺血性心脏病的诊断。

To establish the diagnosis of ischemic heart disease in the early atage, we studied whether cell injuries including cell death were led in vitro during anoxia (or hypoxia) using cultured adult rat cardiomyocytes. In the hypoxic condition of 20 mmHg PO2, morphological changes such as cellular contraction, swelling, disappearance of cross striations and bleb formation were observed up to 4 hr. Creatine phosphokinase contents in the extracellular medium also increased. Moreover, longer period of hypoxic incubation resulted in DNA degradation including DNA fragmentations with 180 bp multiples. These results suggest that these morphological changes and apoptosis in forensic pathological materials may be useful for the diagnosis of ischemic heart disease.