Biochemistry of oil bodies in higher

高等油体的生物化学

• 批准号: 11694086
• 负责人: HIRATA Toshifumi
• 金额: $ 2.94万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11694086/
• 关键词: Oil body; Defense reaction; Carboxypeptidase; Recognition of chemical stress; Cultured plant cells; 植物細胞; オイルボディー; チオールプロテアーゼ; ペルオキシダーゼ

The supramolecule structure and function of oil bodies, which are known as an organelle for accumulation of the materials in the plant cells, were investigated.1. It was expected that the oil body in the shoot primordia of Matricaria chamomilla was constructed as a surrounded monolayer molecule of acylglycerols. The oil bodies contained triacylglycerols as major components, mono- and diacyl glycerols, fatty acids, phospholipids, and steroids.2. The oil body in the shoot primordia of M.chamomilla did not contain any volatile components (essential oils), but contained a small amount of proteins. Proteins containing in the oil body in the seeds of M.chamomilla have a protease-like activity, but those in the oil body of the shoot primordia showed a peptidase-like activity.3. Amino acid sequences of two proteases found in the oil bodies of M.chamomilla was determined by cloning of their corresponding cDNA ; both proteins are constructed by 501 amino acids, and have active cites for serine carboxypeptidase and thiol protease, respectively. It was also observed that mRNAs related to these peptidases were increased by the chemical stress against the cultured cells of M.chamomilla.

研究了油体的超分子结构和功能，油体是植物细胞中积累物质的细胞器。推测母菊茎原基中的油体是以甘油单层分子包围的形式存在的。油体主要成分为三酰甘油、单甘油和双甘油、脂肪酸、磷脂和类固醇。甘菊枝条原基中的油体不含任何挥发性成分(精油)，但含有少量蛋白质。洋甘菊种子油体中含有的蛋白质具有类蛋白酶活性，而地上部原基油体中的蛋白质具有类肽酶活性。通过克隆甘菊油体中发现的两种蛋白的氨基酸序列，确定了它们的氨基酸序列，这两种蛋白均由501个氨基酸组成，分别具有丝氨酸羧肽酶和硫醇蛋白水解酶活性。此外，还观察到与这些多肽酶相关的mRNAs在对甘菊培养细胞的化学胁迫中增加。

项目成果

Shinji Ohta: "Isolation and Identification of Cell Hypertrophy-Inducing Substances in the Gal Forming Aphid Colopha moriokaensis"Insect Biochem.Mol.Biol.. 30・10. 947-952 (2000)

Shinji Ohta：“Gal 形成蚜虫 Colopha moriokaensis 中细胞肥大诱导物质的分离和鉴定” Insect Biochem.Mol.Biol.. 947-952 (2000)。

Toshifumi Hirata: "A 38 kDa Allylic Alcohol Dehydrogenase from the Cultured Cells of Nicotiana tabacum"Phytochemistry. 55. 297-303 (2000)

Toshifumi Hirata：“来自烟草培养细胞的 38 kDa 烯丙醇脱氢酶”植物化学。

Toshifumi Hirata: "A 20-Kda protein mith the GTP-binding and trypsin inhibitory activities from Glycine max"Biosci. Biotechnol. Biochem.. 63・10. 1816-1818 (1999)

Toshifumi Hirata：“来自 Glycine max 的具有 GTP 结合和胰蛋白酶抑制活性的 20-Kda 蛋白质”Biosci. Biochem.. 1816-1818 (1999)。

Chie Kohchi: "Isolation of a CDNA encoding for a carboxypeptidase, haning leucine zipper structure at the N-terminal region, from Matricaria chamomilla"Plant Biotechnol.. 16・5. 409-412 (1999)

Chie Kohchi：“从母菊中分离编码羧肽酶、N-末端区域的哈宁亮氨酸拉链结构的 cDNA”植物生物技术.. 16・5 (1999)。

Chie Kohchi: "Isolation of a cDNA Encoding for a Carboxypeptidase, having Leucine Zipper Structure at the N-terminl Region, from the Cultured Shoot Primordia of Matricaria chamomilla"Plant Biotechnology. 16 (5). 409-412 (1999)

Chie Kohchi：“从母菊培养芽原基中分离编码羧肽酶的 cDNA，其 N 末端区域具有亮氨酸拉链结构”植物生物技术。