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Fundamental and technological studies on the ability of the plant cultured cells to produce and transform organic compounds

植物培养细胞产生和转化有机化合物能力的基础和技术研究

基本信息

批准号：
59840013
负责人：
HIRATA Toshifumi
金额：
$ 5.82万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

项目摘要

The biochemical potentiality of plant cultured cells to produce useful substances is of considerable interest in connection with the use of the cultured cells as a bioreactor. We have investigated the bioconversion of terpenoids with the cultured cells of Nicotiana tabacum for generalizing the bioconversion pattern and the in vitro shoot tip cultures of Zingiber officinale and Mentha spicata for producing the volatile constituents by the cultured cells.(1) The cultured cells were found to have the ability to transform the substrates as follows: the regio- and stereo-selective hydroxylation at the <alpha> -position of carbonyl group, the stereospecific hydrogenation of the C-C double bond adjacent to carbonyl group, and the enantioselective conversion of the hydroxyl, carbonyl, and acetoxyl groups and the C-C double bond.(2) The oxidoreduction between cyclic alcohols and their corresponding ketones in the cultured cells of N. tabacum was governed by an <NAD^+> - dependent alcohol dehydrogenase and the balance in the equilibrium of the oxidoreduction was correlated with the <^(13)C> NMR chemical shift of the carbonyl carbon.(3) The time-courses in the oxidoreduction in the cultured cells can be predicted on the basis of the permeability of the substrates into the cells and the <^(13)C> NMR chemical shifts of the carbonyl carbon.(4) The accumulation of mono- and sesquiterpenoids in the culture plantlets of z. officinale and M. spicata can be controlled by the combination of the ouxin and the basal culture medium.

植物培养细胞产生有用物质的生化潜能与将培养细胞用作生物反应器有关。我们研究了烟草细胞对萜类化合物的生物转化，以推广其生物转化模式，并研究了生姜和薄荷的离体茎尖培养细胞对挥发性成分的产生。(1)培养细胞具有以下转化底物的能力：羰基< α > -位置的区域选择性和立体选择性羟基化，羰基附近的C-C双键的立体特异性氢化，羟基、羰基、乙酰氧基和C-C双键的对映选择性转化。(2)烟叶N. tabacum培养细胞中环醇与相应酮之间的氧化还原受<NAD^+> -依赖的醇脱氢酶控制，氧化还原平衡中的平衡与羰基碳的<^(13)C> NMR化学位移有关。(3)根据底物对细胞的渗透性和羰基碳的<^(13)C> NMR化学位移，可以预测培养细胞中氧化还原的时间过程。(4)乌皮素与基础培养基配合施用可有效控制牛皮草和木皮草培养苗中单萜类和倍半萜类物质的积累。