Interactions between early trophoblast cells and uterine endometrial cells at implantation

研究了人胚胎着床时早期滋养细胞与子宫内膜细胞之间的相互作用。从正常子宫内膜细胞中提取条件培养基，使用明胶作为底物进行酶谱分析。密度分析显示，72KD和92KD明胶酶活性的表达模式随月经周期的变化而变化。在子宫内膜异位症患者的腹水中，金属蛋白酶（MMP）活性比健康妇女的腹水增强，而MMP的组织抑制剂TIMP的浓度未见显著增加。免疫组化分析显示，基底膜细胞外基质（层粘连蛋白、iv型胶原）定位于子宫内膜基底膜基质和间质细胞。人类胎盘产生的各种细胞因子对滋养层细胞生长和分化的影响已经通过体外正常和培养滋养层肿瘤细胞产生hCG来确定。滋养层细胞衍生的肿瘤坏死因子- α (tnf - α)通过IL-6和IL-6受体依赖系统诱导胎盘晚期滋养层细胞释放hCG。添加IL-6可协同促进hCG的产生。然而，tnf - α抑制早期胎盘滋养层细胞和培养的滋养层肿瘤细胞产生hCG。通过添加滋养层细胞衍生的tgf - β，观察到对滋养层细胞hCG产生的类似抑制作用。

Interactions between early trophoblast cells and uterine endometrial cells at implantation of human embryo have been investigated.Conditioned medium from normal endometrial cells was subjected to zymography using gelatin as a substrate. Densitometric analysis showed that the expression pattern of 72KD and 92KD gelatinase activity was varied according to the menstrual cycle. In the ascites fluid obtained from endometriosis patients, metalloproteinase(MMP) activity was enhanced compaired with that from healthy women, while no significant increase was observed in the concentration of TIMP, tissue inhibitor of MMP.Immunohistochemical analysis revealed that extracellular matrix of basement membrane (laminin, typeIV collagen) was localized at the matrix of basement membrance of endometrial gland and stromal cells.The effects of various cytokines produced from human placenta on the growth and differentiation of trophoblast cells have been determined by in vitro hCG production from normal and cultured trophoblastic tumor cells. Trophoblast-derived tumor necrosis factor-alpha(TNF-alpha) induces release of hCG using IL-6 and IL-6-receptor dependent system in the late placental trophoblasts. The hCG production was enhanced synergistically by the addition of IL-6. However, TNF-alpha inhibited hCG production from early placental trophoblasts and cultured trophoblastic tumor cells. Similar inhibition of hCG production of trophoblasts has been observed by the addition of trophoblast-derived TGF-beta.