Interactions between early trophoblast cells and uterine endometrial cells at implantation

着床时早期滋养层细胞与子宫内膜细胞之间的相互作用

基本信息

批准号：
03454397
负责人：
SAJI Fumitaka
金额：
$ 4.16万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454397/
关键词：
Extracellular matrix MMP TIMP cytokine hCG IL-6 TGF-beta TNF-alpha 着床子宮内膜 zymography 胎盤絨毛細胞

项目摘要

Interactions between early trophoblast cells and uterine endometrial cells at implantation of human embryo have been investigated.Conditioned medium from normal endometrial cells was subjected to zymography using gelatin as a substrate. Densitometric analysis showed that the expression pattern of 72KD and 92KD gelatinase activity was varied according to the menstrual cycle. In the ascites fluid obtained from endometriosis patients, metalloproteinase(MMP) activity was enhanced compaired with that from healthy women, while no significant increase was observed in the concentration of TIMP, tissue inhibitor of MMP.Immunohistochemical analysis revealed that extracellular matrix of basement membrane (laminin, typeIV collagen) was localized at the matrix of basement membrance of endometrial gland and stromal cells.The effects of various cytokines produced from human placenta on the growth and differentiation of trophoblast cells have been determined by in vitro hCG production from normal and cultured trophoblastic tumor cells. Trophoblast-derived tumor necrosis factor-alpha(TNF-alpha) induces release of hCG using IL-6 and IL-6-receptor dependent system in the late placental trophoblasts. The hCG production was enhanced synergistically by the addition of IL-6. However, TNF-alpha inhibited hCG production from early placental trophoblasts and cultured trophoblastic tumor cells. Similar inhibition of hCG production of trophoblasts has been observed by the addition of trophoblast-derived TGF-beta.

已经研究了早期滋养细胞细胞与子宫子宫内膜细胞之间在植入人类胚胎时的相互作用。正常子宫内膜细胞的条件培养基使用明胶作为底物进行ZMOGRAGHY。光密度分析表明，根据月经周期，72KD和92KD明胶酶活性的表达模式变化。 In the ascites fluid obtained from endometriosis patients, metalloproteinase(MMP) activity was enhanced compaired with that from healthy women, while no significant increase was observed in the concentration of TIMP, tissue inhibitor of MMP.Immunohistochemical analysis revealed that extracellular matrix of basement membrane (laminin, typeIV collagen) was localized at the matrix of basement membrance of子宫内膜腺体和基质细胞。由人胎盘产生的各种细胞因子对滋养细胞细胞生长和分化的影响已通过从正常和培养的滋养细胞肿瘤细胞中产生的体外HCG产生来确定。滋养细胞衍生的肿瘤坏死因子-Alpha（TNF-Alpha）在晚期胎盘滋养细胞中使用IL-6和IL-6受依赖性系统诱导HCG释放HCG。通过添加IL-6协同增强了HCG的产生。然而，TNF-α抑制了早期胎盘滋养细胞和培养的滋养细胞肿瘤细胞的HCG产生。通过添加滋养细胞衍生的TGF-β，已经观察到了滋养层细胞的HCG产生类似的抑制作用。