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Interactions between early trophoblast cells and uterine endometrial cells at implantation

着床时早期滋养层细胞与子宫内膜细胞之间的相互作用

基本信息

批准号：
03454397
负责人：
SAJI Fumitaka
金额：
$ 4.16万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454397/
关键词：
Extracellular matrix MMP TIMP cytokine hCG IL-6 TGF-beta TNF-alpha 着床子宮内膜 zymography 胎盤絨毛細胞

项目摘要

Interactions between early trophoblast cells and uterine endometrial cells at implantation of human embryo have been investigated.Conditioned medium from normal endometrial cells was subjected to zymography using gelatin as a substrate. Densitometric analysis showed that the expression pattern of 72KD and 92KD gelatinase activity was varied according to the menstrual cycle. In the ascites fluid obtained from endometriosis patients, metalloproteinase(MMP) activity was enhanced compaired with that from healthy women, while no significant increase was observed in the concentration of TIMP, tissue inhibitor of MMP.Immunohistochemical analysis revealed that extracellular matrix of basement membrane (laminin, typeIV collagen) was localized at the matrix of basement membrance of endometrial gland and stromal cells.The effects of various cytokines produced from human placenta on the growth and differentiation of trophoblast cells have been determined by in vitro hCG production from normal and cultured trophoblastic tumor cells. Trophoblast-derived tumor necrosis factor-alpha(TNF-alpha) induces release of hCG using IL-6 and IL-6-receptor dependent system in the late placental trophoblasts. The hCG production was enhanced synergistically by the addition of IL-6. However, TNF-alpha inhibited hCG production from early placental trophoblasts and cultured trophoblastic tumor cells. Similar inhibition of hCG production of trophoblasts has been observed by the addition of trophoblast-derived TGF-beta.

本研究以明胶为底物，对正常子宫内膜细胞的条件培养液进行酶谱分析，探讨了人胚胎着床时早期滋养层细胞与子宫内膜细胞的相互作用。光密度分析表明，72 KD和92 KD明胶酶活性的表达模式因月经周期而异。子宫内膜异位症患者腹水中基质金属蛋白酶（MMP）活性较正常对照组明显升高，而MMP组织抑制剂TIMP的浓度无明显升高，免疫组化结果显示，子宫内膜异位症患者腹水中基质金属蛋白酶（MMP）活性明显升高，基质金属蛋白酶组织抑制剂TIMP的浓度无明显升高，基质金属蛋白酶组织抑制剂TIMP的浓度无明显升高，基质金属蛋白酶组织抑制剂TIMP的浓度无明显升高，基质金属蛋白酶组织抑制剂TIMP的浓度无明显升高。（层粘连蛋白，IV型胶原蛋白）胎盘细胞因子主要定位于子宫内膜腺上皮基底膜基质及间质细胞，本研究探讨了人胎盘细胞因子对子宫内膜腺上皮细胞因子表达的影响。滋养层细胞的生长和分化已经通过从正常和培养的滋养层肿瘤细胞体外产生hCG来确定。滋养层细胞源性肿瘤坏死因子-α（TNF-α）在晚期胎盘滋养层细胞中使用IL-6和IL-6受体依赖性系统诱导hCG释放。通过添加IL-6协同增强hCG的产生。然而，TNF-α抑制早期胎盘滋养层细胞和培养的滋养层肿瘤细胞产生hCG。通过添加滋养层衍生的TGF-β，已经观察到对滋养层hCG产生的类似抑制。