Studies of human trophoblast derived factors which regulate fetal and placental development

调节胎儿和胎盘发育的人类滋养层衍生因子的研究

• 批准号: 05454450
• 负责人: SAJI Fumitaka
• 金额: $ 5.06万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454450/
• 关键词: Trophoblast; Decidua; IL-6; LIF; Apoptosis; Oxytocin receptor; 胎盤絨毛; アポトーシス; IL‐6; hCG; フィブロネクチン; オキシトシン・レセプター

Human placental trophoblast derived factors which regulate fetal and placental development have been investigated at molecular level, and following results were obtained.(1) Interlerukin-6 receptor(IL-6R) complex(gp80 and gp130) is expressed on human trophoblast and is associated with human chorionic gonadotropin. Soluble IL-6R found in serum of pregnant women enhances hCG production via IL-6 and its reeptor system. In addition to IL-6, leukemia inhibitory factor (LIF) which is found in a great amount at fetomaternal interface stimulates hCG production from the trophoblast.(2) Apoptosis in human placental tissue was examine by means of DNA fragmentation. In normal pregnancy, post-term placental trophoblasts clearly showed apoptosis. Futhermore, DNA fragmentation was obtained from placentas of intruterine fetal growth retardation or pregnancy toxemia.The results indicate a close association between apoptosis of trophoblast and placental function.(3) The expression of oxytocin receptor (OTR) on human uterine endometrium was studied at mRNA and protein level. The expression was higher in the secretory phase endometrium than in the proliferative endometrium. In situ hybridization showed the localization of OTR at endometrial gland tissue. The data suggests the relation between inplantation and OTR expression in the endometrium.

人类胎盘滋养细胞衍生因子在分子水平上调控胎儿和胎盘发育，并获得了以下结果。(1)白细胞介素-6受体（IL-6R）复合物（gp80和gp130）在人滋养细胞上表达，与人绒毛膜促性腺激素相关。孕妇血清中发现可溶性IL-6R通过IL-6及其受体系统促进hCG的产生。除IL-6外，大量存在于胎母界面的白血病抑制因子（leukemia inhibitory factor， LIF）刺激滋养细胞产生hCG。(2)采用DNA片段法检测人胎盘组织的凋亡。在正常妊娠中，胎盘滋养细胞明显凋亡。此外，从宫内胎儿生长迟缓或妊娠毒血症的胎盘中获得DNA片段。结果提示滋养细胞凋亡与胎盘功能密切相关。(3)从mRNA和蛋白水平研究了人子宫内膜催产素受体（OTR）的表达。分泌期子宫内膜的表达高于增殖期子宫内膜。原位杂交显示OTR定位于子宫内膜腺组织。这些数据提示着床与子宫内膜中OTR表达之间的关系。

项目成果

Fumitaka Saji et al.: "Current Topic:Human Placental Fc receptors." Placenta. 15. 453-466 (1994)

Fumitaka Saji 等人：“当前主题：人胎盘 Fc 受体。”

Toshihito Kanai et al.: "Increased level of interleukin-1 receptor antagonist(IL-1ra)in uterine cervical mucus at ovulatory phase." Proceedings of the 8th Annual Meeting of JSIR 1994.8. 17 (1994)

Toshihito Kanai 等人：“排卵期宫颈粘液中白介素 1 受体拮抗剂 (IL-1ra) 水平升高。”

Toushun Jo et al.: "Apoptosis of cultured mouse luteal cells induced by tumor necrosis factor-alpha and interferon-gamma" The AnatomicalRecord. 241. 70-76 (1995)

Tooushun Jo 等人：“肿瘤坏死因子-α 和干扰素-γ 诱导培养的小鼠黄体细胞凋亡”解剖记录。

Toshun Jo et al: "Inhibitory effects of estrogen,progesterone,androgen and glucocorticoid on death of neonatal mouse uterine epithelial cells induced to proliferate by estrogen." J.Steroid Biochem.Molec.Biol.46. 25-32 (1993)

Toshun Jo等人：“雌激素、孕激素、雄激素和糖皮质激素对雌激素诱导增殖的新生小鼠子宫上皮细胞死亡的抑制作用”。

Masahiko Takemura et al.: "Expression and localization of human oxytocin receptor mRNA and its protein in chrion and decidua during parturition." J.Clin. Invest.241. 2319-2323 (1994)

Masahiko Takemura 等人：“分娩过程中人催产素受体 mRNA 及其蛋白质在绒毛膜和蜕膜中的表达和定位。”