Studies of human trophoblast derived factors which regulate fetal and placental development

本研究从分子水平对调节胎儿和胎盘发育的人胎盘滋养层细胞衍生因子进行了研究，结果表明：(1)人滋养层细胞表达白细胞介素6受体(IL-6R)复合体(gp80和gp130)，并与绒毛膜促性腺激素相关。孕妇血清中发现的可溶性IL-6R通过IL-6及其受体系统促进hCG的产生。除IL-6外，母胎界面大量存在的白血病抑制因子(LIF)可刺激滋养层细胞产生hCG。(2)DNA片段法检测人胎盘组织中的细胞凋亡。在正常妊娠中，足月胎盘滋养层细胞明显出现凋亡。结果表明，滋养细胞的凋亡与胎盘功能密切相关。(3)从基因和蛋白水平研究了催产素受体在子宫内膜的表达。在分泌期子宫内膜中的表达高于增生期。原位杂交显示OTR定位于子宫内膜腺组织。提示着床与OTR在子宫内膜的表达有一定的关系。

Human placental trophoblast derived factors which regulate fetal and placental development have been investigated at molecular level, and following results were obtained.(1) Interlerukin-6 receptor(IL-6R) complex(gp80 and gp130) is expressed on human trophoblast and is associated with human chorionic gonadotropin. Soluble IL-6R found in serum of pregnant women enhances hCG production via IL-6 and its reeptor system. In addition to IL-6, leukemia inhibitory factor (LIF) which is found in a great amount at fetomaternal interface stimulates hCG production from the trophoblast.(2) Apoptosis in human placental tissue was examine by means of DNA fragmentation. In normal pregnancy, post-term placental trophoblasts clearly showed apoptosis. Futhermore, DNA fragmentation was obtained from placentas of intruterine fetal growth retardation or pregnancy toxemia.The results indicate a close association between apoptosis of trophoblast and placental function.(3) The expression of oxytocin receptor (OTR) on human uterine endometrium was studied at mRNA and protein level. The expression was higher in the secretory phase endometrium than in the proliferative endometrium. In situ hybridization showed the localization of OTR at endometrial gland tissue. The data suggests the relation between inplantation and OTR expression in the endometrium.