Analysis of the Reproductive Failure by Using Monoclonal Antibodies Against Human Sperm

抗人精子单克隆抗体导致生殖障碍的分析

• 批准号: 01570929
• 负责人: SAJI Fumitaka
• 金额: $ 1.34万
• 依托单位: Osaka University Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570929/
• 关键词: Antisperm antibody; Infertility; Capacitation; ELISA; Sperm immobilizing test; monoclonal antibody; acrosomal reaction; capacitation

1. Screening tests for antisperm antibodies were performed on 684 infertile women by Sperm Agglutination test (SAT), sperm immobilization test (SIT), enzyme-linked immunosorbent assay (ELISA), immunobeads test and mixed antiglobulin reaction (MAR). Seventy-one patients with circulating antisperm antibodies were selected by these methods.2. The monoclonal antibodies against human sperm were obtained by using Balb/c mouse spleen cells immunized with capacitated human sperm. All antibodies showed inhibitory effects on Zona free hamster egg-human sperm penetration test (ZSPT).3. Monoclonal antibody, MH61, which reacts specifically with capacitated human sperm was conjugated with Dynabeads M-450. A remarkable agglutination of MH61 coated beads was observed after incubation with sperm of fertile men, while poor agglutination activity was observed in sperm of infertile patients.

1.通过精子凝集试验（SAT）、精子固定试验（SIT）、酶联免疫吸附试验（ELISA）、免疫珠试验和混合抗球蛋白反应（MAR）对684名不孕妇女进行抗精子抗体筛查试验。通过这些方法筛选出71例循环中有抗精子抗体的患者。 2．抗人精子的单克隆抗体是通过使用具有获能的人精子免疫Balb/c小鼠脾细胞获得的。所有抗体对无带仓鼠卵-人精子穿透试验（ZSPT）均表现出抑制作用。3．单克隆抗体 MH61 与获能的人类精子发生特异性反应，并与 Dynabeads M-450 结合。 MH61包被的珠子与有生育能力的男性精子孵育后观察到显着的凝集活性，而在不育患者的精子中观察到较差的凝集活性。

项目成果

Kazutomo ohashi, Munehiro Kato, Takao Negoro, Fumitaka Saji, and Osamu Tanizawa: "HLA expression of sperm cell surface" Acta. Obstet. Gynec. Jpn. 41(4). 497-498 (1989)

Kazutomo ohashi、Munehiro Kato、Takao Negoro、Fumitaka Saji 和 Osamu Tanizawa：“精子细胞表面的 HLA 表达”Acta。

Akinari Wakimoto,Munehiro Kato,Kazutomo Ohashi,Fumitaka Saji,and Osamu Tanizawa.: "Weak expression of HLA class II on human sperm" J.Reprol.Immunol.Suppl. 55 (1989)

Akinari Wakimoto、Munehiro Kato、Kazutomo Ohashi、Fumitaka Saji 和 Osamu Tanizawa.：“人类精子上 HLA II 类的弱表达”J.Reprol.Immunol.Suppl。

大橋 一橋,根来 孝夫,佐治 文隆,谷澤 修: "精子不動化抗体陽性不妊婦人血清,卵胞液と特異的に反応するヒト精子細胞膜抗原の同定" 日本産科婦人科学会誌. 41(4). 467-472 (1989)

Hitotsubashi Ohashi、Takao Negoro、Fumitaka Saji、Osamu Tanizawa：“与精子固定抗体阳性不孕女性血清和卵泡液特异性反应的人类精子细胞膜抗原的鉴定”日本妇产科学会杂志41（4）。 467-472（1989）

Kazutomo Ohashi.Munehiro Kato,Takao Neyoro,Fumitaka Saji,and Osamu Tanizawa: "HLA Expressin of Sperm Cell Surface" 日本産婦人科学会誌. 41(1). 1-6 (1989)

Kazutomo Ohashi.Munehiro Kato、Takao Neyoro、Fumitaka Saji 和 Osamu Tanizawa：“精子细胞表面的 HLA 表达”，日本妇产科学会杂志 41(1) (1989)。

Munehiro Kato: "Evaluation of sperm fertilizing ability with monoclonal antibody specific to acrosomeーreacted sperm" Proceeding of the 5th annual meeting of Japan society for medical reproductive Immunology in press. (1990)

加藤宗宏：“用顶体反应精子特异性单克隆抗体评估精子受精能力”，日本医学生殖免疫学会第五届年会论文集（1990年）。