Molecular analysis of fertilizing ability in human spermatozoa

人类精子受精能力的分子分析

• 批准号: 07671780
• 负责人: SAJI Fumitaka
• 金额: $ 1.34万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671780/
• 关键词: human spermatozoa; fertilizing capacity; CD46; acrosome reaction; acrobeads test; 精子; 受精; 細胞融合; 男性不妊

The mechanism of mammalian fertilization remains unknown. We focused on CD46 molecule on human spermatozoa after acrosome reaction. We developed Acrobeads test for diagnosing the fertilizing capacity of human spermatozoa (Fertil Steril 63 ; 625-630,1995). We examined 210 semen samples from male partners of infertile couples in in vitro fertilization (IVF) program at 20 university hospital in Japan (Jpn J Fertil Steril 40 ; 368-373,1995) and demonstrated the usefulness for assessing the fertilizing capacity of human spermatozoa in IVF.To determine the role of CD46 on human spermatozoa, we used anti-CD46 antibodies which recognize different epitopes, short consensus sequence (SCR) -1,2, and -3. Anti-SCR-3 antibodies prevented human spermatozoa from penetrating zona-free hamster oocytes. These results suggested that SCR-3 of CD46 on human ejaculated spermatozoa may participate in human fertilization.We also developed a new culture medium containing amino acids in human follicular fluid, and achieved the improvement of mouse embryonic development.

哺乳动物受精的机制尚不清楚。我们对人类精子顶体反应后的CD46分子进行了研究。我们开发了Acrobeads试验来诊断人类精子的受精能力(Fertil Steril 63；625-630,1995)。我们在日本20所大学医院检查了210例体外受精(IVF)不育夫妇的男性伴侣的精液样本(Jpn J Fertil Steril 40；368-373,1995)，并证明了CD46在体外受精中评估人类精子受精能力的有效性。抗SCR-3抗体阻止人类精子穿透去透明带的仓鼠卵母细胞。这些结果提示人射精精子表面CD46的SCR-3可能参与了人类受精。我们还开发了一种新的含有人类卵泡液中氨基酸的培养液，并实现了对小鼠胚胎发育的改善。

项目成果

Toshikatsu Nobunaga et al.: "Elevated nitric oxide concentration in the seminal plasma of infertile males" A J Reprod Immunol. 36. 193-197 (1996)

Toshikatsu Nobunaga 等人：“不育男性精浆中一氧化氮浓度升高”A J Reprod Immunol。

谷澤 修 他: "ヒト精子受精能検査試薬FAB-25(アクロビーズテスト)の有用性に関する多施設〓" 日本不妊学会雑誌. 40(3). 368-373 (1995)

Osamu Tanizawa 等：“关于人类精子生育力测试试剂 FAB-25（Acrobeads 测试）有效性的多中心研究”日本不孕协会杂志 40(3) 368-373 (1995)。

Osamu Tanizawa et al.: "Multicenter study of FAB-25, a test kit of the acrobeads test, to evaluate the fertilizing capacity of human spermatozoa" Jpn J Fertil Steril. 40(3). 368-373 (1995)

Osamu Tanizawa 等人：“FAB-25 的多中心研究，FAB-25 是一种 acrobeads 测试试剂盒，用于评估人类精子的受精能力”Jpn J Fertil Steril。

Kazutomo Ohashi et al.: "Acrobeads Test A new diagnostic test for assesment of the fertilizing capacity of human spermatozoa" Fertility and Sterility. 63. 625-630 (1995)

Kazutomo Ohashi 等人：“Acrobeads 测试用于评估人类精子受精能力的新诊断测试”生育力和不育。

Kazutomo Ohashi et al.: "Acrobeads Test : A new diagnostic test for assesment of the fertilizing capacity of fuman spermatozoa" Fertil and Steril. 63. 625-630 (1995)

Kazutomo Ohashi 等人：“Acrobeads 测试：一种用于评估 fuman 精子受精能力的新诊断测试”Fertil 和 Steril。