Molecular analysis of fertilizing ability in human spermatozoa

哺乳动物受精的机制尚不清楚。我们重点研究了顶体反应后CD46分子对人精子的影响。我们开发了用于诊断人类精子受精能力的Acrobeads试验（Fertil Steril 63; 625-630,1995）。我们检查了日本20所大学医院体外受精（IVF）项目中来自不育夫妇男性伴侣的210份精液样本（Jpn J Fertil Steril 40; 368-373,1995），并证明了在体外受精中评估人类精子受精能力的有效性。为了确定CD46在人类精子中的作用，我们使用了识别不同表位、短一致序列(SCR) -1、2和-3的抗CD46抗体。抗scr -3抗体阻止人类精子穿透无带的仓鼠卵母细胞。这些结果提示，人射精精子上CD46的SCR-3可能参与了人类受精。我们还在人卵泡液中开发了一种新的含有氨基酸的培养基，并实现了小鼠胚胎发育的改善。

The mechanism of mammalian fertilization remains unknown. We focused on CD46 molecule on human spermatozoa after acrosome reaction. We developed Acrobeads test for diagnosing the fertilizing capacity of human spermatozoa (Fertil Steril 63 ; 625-630,1995). We examined 210 semen samples from male partners of infertile couples in in vitro fertilization (IVF) program at 20 university hospital in Japan (Jpn J Fertil Steril 40 ; 368-373,1995) and demonstrated the usefulness for assessing the fertilizing capacity of human spermatozoa in IVF.To determine the role of CD46 on human spermatozoa, we used anti-CD46 antibodies which recognize different epitopes, short consensus sequence (SCR) -1,2, and -3. Anti-SCR-3 antibodies prevented human spermatozoa from penetrating zona-free hamster oocytes. These results suggested that SCR-3 of CD46 on human ejaculated spermatozoa may participate in human fertilization.We also developed a new culture medium containing amino acids in human follicular fluid, and achieved the improvement of mouse embryonic development.