Structure and function of platelet membrane glycoproteins-analysis of their mechanism using gene analysis and affinity labelling

血小板膜糖蛋白的结构和功能-利用基因分析和亲和标记分析其机制

• 批准号: 61580149
• 负责人: MOROI MASAAKI
• 金额: $ 1.34万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61580149/
• 关键词: Platelet; glycoprotein; GPIb; GPII b; polymorphism; thrombosis; biosynthesis; 血小板無力症; GP【I】b; GP【II】b; グリコカリシン

1. Polymorphism of platelet membrane glycoprotein Ib and its physiological function-Glycocalicin, a degraded form of GPIb,was purified and further degraded by various kinds of proteases. polymorphism of GPIb was found to be derived from glycopeptide portion of 90kDa. Polymorphysm of GPIb of patients with brain thombosis was analyzed. Although number is rather small(n=18), type C GPIb was found to be more and type D was found to be less in the patient than in the normal Japanese subjects. The relationship between the occurence of thrombosis and type C GPIb should be confirmed by using a larger number of patients.2. Biosynthesis of platelet glycoproteins-Megakaryoblast cell line was found to express GPIb,II b and IIIa antigens. Precursors of GPIb and GPII b were found in the different subfractions from plasma membrane fraction.3. Thrombasthenia with precursor type GPII b-We found 2 different families of patients with thrombasthenia who have an abnormal GPII b with larger molecular weight. This abnormal GPII b was identified as a precrsor of GPII b which was mentioned above. Analysis of the abnormal GPII b in these patients would give us useful infomation for understanding the mechanism of biosynthesis of GPII b and IIIa.

1.血小板膜糖蛋白Ib的多态性及其生理功能--糖杯素是血小板膜糖蛋白Ib的降解形式，经纯化后可被多种蛋白酶进一步降解。GPIb的多态性来源于90 kDa的糖肽部分。分析脑血栓患者GPIb的多态性。虽然数量相当少（n=18），C型GPIb被发现更多，D型被发现在患者比正常的日本人少。血栓的发生与C型GPIb的关系需要大量的病例来证实.血小板糖蛋白生物合成-巨核细胞系表达GPI B、II B和IIIa抗原。GPI B和GPII B的前体存在于质膜组分的不同亚组分中.血小板无力症伴前体GPII b型-我们发现2个不同的血小板无力症患者家族具有较大分子量的异常GPII B。这种异常的GPII B被鉴定为上述GPII B的前体。对这些患者异常GPII B的分析，为进一步了解GPII B和IIIa的生物合成机制提供了有益的信息。

项目成果

S.M.Jung et al: Thromb.Res.42. 83-90 (1986)

S.M.Jung 等人：Thromb.Res.42。

Jung,S.M.: Blood. 71. (1988)

Jung,S.M.：血。

Jung, S. M., Yoshida, N., Aoki, N., Tanoue, K., Yamazaki, H. and Moroi, M.: "Thrombasthenia with an abnormal platelet membrane glycoprotein of different molecular weight" Blood. 71. (1988)

Jung，S.M.，Yoshida，N.，Aoki，N.，Tanoue，K.，Yamazaki，H.和Moroi，M.：“具有不同分子量的异常血小板膜糖蛋白的血小板无力症”血液。