Development of a multi-agent system for plant biocommunication

植物生物通讯多智能体系统的开发

• 批准号: 07506003
• 负责人: TAKAKURA Tadashi
• 金额: $ 8.51万
• 依托单位: Nagasaki University (1997)The University of Tokyo (1995-1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07506003/
• 关键词: Stable isotope; Photosynthesis; Respiration; LIF; Laser irradiation; レーザー照射; 同時測定

It is essential to mesure precisely direct reaction from plants, in order to achieve the maximum production and highest quality of plants in a plant factory where stable and high efficiency production is required. However, it has not yet been cleared that the relationship between plant physiology and its controlled environments. The purpose of the present study is to develop a multi-agent system to measure plant responses in several aspects. Using stabel isotopes, the simultaneous measurement of photosynthesis and respiration has been developed. Furthemore, the plant fluoresence response induced by a laser beam has been analyzed and temperature pattern of leaves by thermograph was used to detect water stress or plants.A leaf chamber has been built and corn and pothos leaves have been tested by using a stable isotope ^<13>CO_2. The separation of photosynthesis and respiraton, that is, simultaneous measurement of photosynthesis and respiration was conducted, assuming the photosyntheis product using ^<13>CO_2 did not occurer. From this method, photoresipiration was clearly shown. Using the same system, the measurement of potato and sweet potato has been conducted to find net and gross photosynthesis. The measurement of gross photosynthesis has not been completely cleared.The system to detect laser induced fluorescence from plants was developed. Using spinach plants, it has been cleared that a correlation between decreasing values of the ratio of chlorophyll a/b and plant age. Water stress of tomato seedlings was tested. The peak responses at 685nm and 740nm show some trend but it is not clear.Water stress of melon seedlings was measured by a thermograph which gave temperature pattern of the leaves. Green component of the pattern was also analyzed. Temperature in crease and G-value decrease are well corelated and they showed water stress tendency.

为了在植物工厂中实现植物的最大产量和最高质量，需要稳定和高效的生产，必须精确测量植物的直接反应。然而，植物生理与其受控环境之间的关系尚未明确。本研究的目的是开发一个多智能体系统来测量植物在几个方面的反应。利用稳定同位素，发展了光合作用和呼吸作用的同时测定。此外，还分析了激光诱导植物的荧光响应，并利用温度计测量的叶片温度曲线来检测植物的水分胁迫<13>。光合作用与呼吸作用的分离，即光合作用与呼吸作用的同时测定，是在假定利用CO_2的光合产物<13>不存在的情况下进行的。从这种方法中，光致抗蚀剂被清楚地显示。利用相同的系统，对马铃薯和甘薯进行了测定，以确定净光合作用和总光合作用。植物光合作用总量的测量还没有完全解决，我们研制了一套激光诱导荧光检测系统。使用菠菜植物，已经清楚的是，叶绿素a/B的比率的降低值与植物年龄之间的相关性。对番茄幼苗进行了水分胁迫试验。在685 nm和740 nm处的峰值响应有一定的变化趋势，但变化不明显。还分析了图案的绿色分量。温度升高与G值下降相关性较好，表现出水分胁迫的趋势。

项目成果

二見 順, 西 功, 後藤 英司, 高倉 直: "^<13>CO_2を用いた植物の光合成・呼吸同時分別測定システムの開発" CELSS学会. (発表予定).

Jun Futami、Isao Nishi、Eiji Goto、Nao Takakura：“利用 ^<13>CO_2 开发植物光合作用和呼吸作用的同步分馏测量系统”CELSS 协会（已安排演示）。

Nishi, I., Furuta, J., Ma, P., Ishii, H., Takakura, T., and Goto, E.: "Discrimination and continuous measurement of photosynthesis and respiration by monitoring ^<13>CO_2 and ^<12>CO_2 as tracers." Acta Hort.440. 486-491 (1996)

Nishi, I.、Furuta, J.、Ma, P.、Ishii, H.、Takakura, T. 和 Goto, E.：“通过监测 ^<13>CO_2 和 ^< 来区分和连续测量光合作用和呼吸作用

Norikane, J., Goto, E.and Takakura, T.: "Plant stress analysis by LIF.Agricultural Meteorological Society Annual Meeting" (scheduled).

Norikane, J.、Goto, E. 和 Takakura, T.：“LIF 植物胁迫分析。农业气象学会年会”（预定）。

I.Nishi: "Discriminating and continuous measurement of photosynthesis and respiration by monitoring ^<13>CO_2 and ^<12>CO_2 astracer" Acta Horticulturae. 440. 486-491 (1996)

I.Nishi：“通过监测^<13>CO_2和^<12>CO_2 astracer来区分和连续测量光合作用和呼吸”Acta Horticulturae。

Goto, E., Takakura, T., and Okamura, N.: "Development of photosynthesis and respiration measurement system using mass spectrometry." Acta Hort.440. 464-468 (1996)

Goto, E.、Takakura, T. 和 Okamura, N.：“利用质谱法开发光合作用和呼吸测量系统。”