Difference in the uptake of glucose in various hippocampal regions

海马各区域葡萄糖摄取的差异

• 批准号: 62570031
• 负责人: SHIMADA Masahisa
• 金额: $ 1.22万
• 依托单位: Kagawa Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570031/
• 关键词: microautoradiography; 2-deoxyglucose; glucose; GFA; hippocampus; マウス; ABC-DAB法; cell counting; 2-デオキシグルコース

By freeze-mount microautoradiography, differences in the uptake of tracers from radioactive 2-deoxyglucose ([1,2-^3H],[2,6-^3H]) and glucose ([1-^3H],[3-^3H]) into hippocampal regions were investigated after 45 minutes for 2-deoxyglucose(2-DG), and 15 and 45 minutes for glucose. Silver grains were assessed quantitatively by the image analyzer.(1) The radioactivity (silver grains/mm^2) in the stratum lacunosum-moleculare of the Ammon's horn from 2-DG autoradiograms was significantly higher than that of other hippocampal regions (p<0.01), while lowest in the hilus fascia dentata (p<0.01).(2) autoradiograms of [1-^3H] glucose and 15-min of [3-^3H] glucose showed that the radioactivity in the dentata molecular layer was significantly higher than that of other regions, expect for the stratum lacunosum-moleculare (p<0.05).(3) The 2-DG and 45-min glucose autoradiograms showed the intensely labeled perikarya of pyramidial cells in the CA3a sector.(4) Radioactivity in the dentate granular layer from 45-min autoradiogram of [3-^3H] glucose was significantly higher than that of the molecular layer (p<0.05).These results imply that the metabolic fate of glucose, i.e., whether it is mainly used for energy production or amino acid synthesis depends on each structure of the hippocampus. On the other hand, in order to investigate the factors responisible for glucose uptake, districution of synapses and GFA-positive glia in each hippocampal region was quantitatively observed using immnohistochemical preparations. As the results it was found that the radioactivity from 2-DG autoradiograms was closely related to the distribution of GFA-positive glia. i.e. the higher the grain density on an autoradiogram, the greater the glial density in that region.

通过冰冻切片显微放射自显影术，研究了海马区对放射性2-脱氧葡萄糖（[1，2-^3H]，[2，6-^3H]）和葡萄糖（[1-^3H]，[3-^3H]）的示踪剂摄取的差异，2-脱氧葡萄糖（2-DG）在摄取45分钟后，葡萄糖在摄取15和45分钟后。通过图像分析仪定量评估银颗粒。(1)阿蒙角陷窝-分子层的放射性（银粒/mm ^2）显著高于海马其他区域（p<0.01），而齿状筋膜门最低（p<0.01）。(2)[1-^3H]葡萄糖和[3-^3H]葡萄糖15分钟放射自显影结果显示，除腔隙分子层外，齿状层分子层的放射性显著高于其他区域（p<0.05）。(3)2-DG和45分钟葡萄糖放射自显影显示CA 3a区的线粒体细胞胞体呈强标记。(4)[3-^3H]葡萄糖45分钟放射自显影齿状颗粒层的放射性显著高于分子层（p<0.05）。这些结果表明，葡萄糖的代谢归宿，即，海马体主要用于产生能量还是合成氨基酸，取决于海马体的每个结构。另一方面，为了研究葡萄糖摄取的因素，在每个海马区的突触和GFA阳性胶质细胞的分布进行了定量观察，使用免疫组织化学制剂。结果发现，2-DG放射自显影的放射性与胶质细胞的分布密切相关。即放射自显影图上的颗粒密度越高，该区域中的神经胶质密度越大。