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Morphological investigations of glucose uptake in the mouse hippocampus.

小鼠海马葡萄糖摄取的形态学研究。

基本信息

批准号：
02670032
负责人：
SHIMADA Masahisa
金额：
$ 1.47万
依托单位：
Osaka Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

In recent years, there has been a growing interest in the relationships between energy metabolism and neural activity in the brain. We have been investigating the factors responsible for glucose uptake in the mouse hippocampus. We will report here the densities of microvessel, laminine, insulin, insulin receptor and glucose transporter in each laminar region of the hippocampus. The highest density of perfused microvessels with indian ink was found in the stratum lacunosum - moleculare, as compared with other regions, and lowest values were found in the stratum lucidum and dentate granular cell layer. Among pyramidal cell layers, microvessel density of the sector CA3a was significantly higher than that of the CA1. Although laminine distributions stained immunohistochemically were consistent with that of microvessels, immuno - positive reactions were also seen in the pyramidal and granular cell layer. By contraries, insulin and insulin receptors were intensively distributed in the stratum granulare and pyramidale, while they were lowest in the stratum lacunosum - moleculare, suggesting that the insulin have no dealings with the glucose transport into the brain. The immunohistochemical reactions of glucose transporter, responsible for facilitated glucose transport in the brain, were not only highly positive in the microvessel wall, but also in the parenchymal tissue in the stratum lacunosum - moleculare. The severe anemic hypoxia(NO_2 for30 minutes), used for the model of aging, did not any influence to the synaptic densities and morphological changes of astroglial cells in the hippocampus.

近年来，人们对大脑中能量代谢和神经活动之间的关系越来越感兴趣。我们一直在研究小鼠海马体中葡萄糖摄取的因素。我们将在这里报告的微血管密度，laminine，胰岛素，胰岛素受体和葡萄糖转运蛋白在海马的每个层区域。与其他区域相比，用印度墨水灌注的微血管密度最高的是腔隙-分子层，最低的是透明层和齿状颗粒细胞层。在锥体细胞层中，CA 3a区的微血管密度显著高于CA 1区。免疫组化染色结果显示，层粘连蛋白的分布与微血管的分布一致，但在锥体细胞层和颗粒细胞层也可见免疫阳性反应。而胰岛素和胰岛素受体在颗粒层和髓质层分布较集中，在腔隙分子层分布最少，提示胰岛素与葡萄糖的脑内转运无关。葡萄糖转运蛋白的免疫组织化学反应不仅在脑内的微血管壁呈高阳性，而且在腔隙分子层的实质组织中也呈高阳性。衰老模型用严重缺氧（NO_230分钟）对海马星形胶质细胞突触密度和形态学改变无影响。