Possible remodeling of the callosal fibers affected by the callosotomy

受胼胝切开术影响的胼胝纤维可能发生重塑

• 批准号: 07670041
• 负责人: SHIMADA Masahisa
• 金额: $ 1.47万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670041/
• 关键词: motor cortex; callosotomy; pyramidal neuron; dendritic spine; Golgi-impregnation11; レーザー走査型顕微鏡; Golgi-Cox鍍銀染色; レーザー顕微鏡; 維体細胞; シナプス密度; Golgi-Cox

Changes in the number of spines on apical dendrites of pyramidal neurons in layrs III and V of the motor area of the cerebral cortex were examined in the young adult mice (30-60 days old) by a modified Golgi-Cox method and laser scanning microscopy on the 1st, 6th, 10th and 12th days after callosotomy. The anterior part of the callosal body, including rostrum, genu and truncus corporis callosi, was sectioned with a razor blade. The numbers of spines on apical dendrites and their oblique branches of pyramidal neurons were counted at the level of layr II for layr III pyramidal neurons and at the level of layr III for layr V pyramidal neurons. The density of spines was increased on all these dendritic parts during 10 days after callosotomy ; the diameters of spine stems and dendrites were also increased. There changes, however, appeared to be transient as they were decreased almost to normal level on the 12th day after callosotomy.

用改良Golgi-Cox法和激光扫描显微镜观察了30-60日龄幼龄小鼠胼胝体切开后第1、6、10和12天大脑皮质运动区Ⅲ、Ⅴ层锥体神经元顶树突棘数的变化。用刀片将胼胝体前部包括喙部、胼胝体干和胼胝体干切开。在第II层和第III层水平分别计数锥体神经元顶树突及其斜支上棘的数目。胼胝体切开后10天内，树突棘密度增加，棘干和树突直径也增加。但这些变化似乎是短暂的，在胼胝体切开术后第12天几乎降至正常水平。

项目成果

Masahisa Shimada: "Alteration of apical spine density of layer V pyramidal neurons after callosal transection" Brain Research. (発表予定).

Masahisa Shimada：“胼胝体横断后第五层锥体神经元顶棘密度的改变”大脑研究（待提交）。

M.Shimada, et al.,: "Changes in the spine density in the motor area of the cerebral cortex after callosotomy:A study by modified Golgi-Cox method in the mouse." Acta Anat.Nippon.(Kaibougaku-zasshi). 72・6. 545-552 (1997)

M.Shimada 等人：“胼胝体切开术后大脑皮层运动区脊柱密度的变化：通过改良高尔基-考克斯法对小鼠进行的研究。(Kaibougaku-zasshi) ）72·6。

Shimada, M., Negi, T., Itano, T., Tamayama, T.and Murakami T.H.: "Forelimb muscle contraction induced by cerebral cortical stimulation after callosotomy : A myographic study in the mouse." Acta Anat.Nioopn. (Kaibogaku-zasshi). 72(3). 215-218 (1997)

Shimada, M.、Negi, T.、Itano, T.、Tamayama, T. 和 Murakami T.H.：“胼胝体切开术后大脑皮层刺激引起的前肢肌肉收缩：小鼠肌动描记研究。”

M.Shimada et al.: "Forelimb muscle contraction induced by cerebral cortical stimulation after collosotomy:A myographic study in the mouse." Acta Anat.Nippon.(Kaibougaku-Zasshi). 72・3. 215-218 (1997)

M.Shimada 等人：“结肠切开术后大脑皮质刺激引起的前肢肌肉收缩：小鼠肌动描记研究”（Kaibougaku-Zasshi） 72・3（1997）。

Shimada, M., Negi, T., Itano, T., Hayasaki, H., Konishi, M., Watanabe, M.and Murakami, T.H.: "Changes in the spine density of apical dendrites of pyramidal neurons in the motor area of the cerebral cortex after callosotomy : A study by a modified Golgi-Co

Shimada, M.、Negi, T.、Itano, T.、Hayasaki, H.、Konishi, M.、Watanabe, M. 和 Murakami, T.H.：“运动区锥体神经元顶端树突棘密度的变化