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Analysis of avian retinotectal projection.

鸟类视网膜顶盖投影分析。

基本信息

批准号：
62570029
负责人：
SATO Akinaro G.
金额：
$ 1.22万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

The alar plate of the prosencephalon of the guail embryo was haterotopically transplanted into the alar plate of the mesencephalon of the chick embryo at the 7-10 somite stage. When the transplant was completely integrated into the host mesencephalon, the transplant differentiated into the laminar structures resmbling to the optic tectum. Immunohistochemistry with MAb-39B11, which recognizes a species-specific antigen on chick never fibers, showed that the host optic nerve fibers innervated both in the host and the transplant when the transplant was integrated into the host, but that optic nerve fibers did not invade the transplant when it had not a laminar pattern of the tectum. Our study suggests that the prosencephalon has the capacity to differentiate into the optic tectum at the 7-10 somite stage.Next, we studied in E9 to E18 chicks the development of topographic order of the projection from temporal retina to rostral optic tectum using the anterograde fluorescent tracers DiI and … More DiO. The trajectories and branching patterns were studied whole mounts of retina and tectum.Retinal fibers enter the contralateral optic tectum at its rostral edge and grow caudally. We labeled the temporal retinal fibers. They terminate on the rostral edge of the tectum. Many axons grow caudally and mediolaterally beyond the expected terminal zone. A small number of overshooting axons reverse course 180゜ and grow back to the terminal zone. After considerable elongation, an axon develops numerous side branches and arbors both in and outside of the appropriate terminal zone. Aberrant arbors and axon collaterals that fail to arborize in the appropriate terminal zone are rapidly eliminated over about a 2 day period. These events lead to the establishment by E16 of the discrete and highly focused zone o fterminal arborization characteristio of the mature projection. The topographic projection is very precise, leaving course corrections along mediolateral and rostrocaudal tectal axis after maturation. Less

将7-10体节期的鹌鹑胚胎前脑的前脑板异位移植到鸡胚中脑的前脑板上。当移植物完全整合到宿主中脑中时，移植物分化为与视顶盖相似的层状结构。免疫组织化学与单克隆抗体-39 B11，它承认鸡神经纤维的种属特异性抗原，表明宿主视神经纤维支配的主机和移植物时，移植物整合到主机，但视神经纤维不侵入移植物时，它没有层状模式的顶盖。我们的研究表明，前脑在7-10体节期具有分化为视顶盖的能力。其次，我们在E9-E18鸡中，用顺行荧光示踪剂DiI和 ...更多信息迪奥视网膜纤维从对侧视顶盖的吻侧缘进入视顶盖，向尾侧生长。我们标记了颞侧视网膜纤维。它们终止于顶盖的嘴侧边缘。许多轴突在预期的终末区之外向尾侧和内侧生长。少数过冲的轴突将路线逆转180度，并长回终末区。在相当长的长度后，轴突在适当的终末区内外都发育出许多侧枝和乔木。在适当的终末区未能形成树枝状的异常树枝和轴突侧枝在约2天的时间内迅速消除。这些事件导致E16建立了成熟投射的离散和高度集中的终末树枝化区。地形投影是非常精确的，留下路线校正沿着内侧和吻尾顶盖轴成熟后。少