Application of gas chromatography/negative ion chemical ionization mass spectrome try to legal medicine

气相色谱/负离子化学电离质谱在法医学中的应用尝试

• 批准号: 62570263
• 负责人: SUZUKI Osamu
• 金额: $ 1.15万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570263/
• 关键词: Mass spctrometry; Negative ion chemical ionization; Toxicology; 薬毒物分析; ガスクロマトグラフィー; 質量分析法; 法中毒学; ベンゾジアゼピン系薬剤

Some advantages in the use of negative ion chemical ionization (NICI) mass spctrometry (MS)have been reported,but they depend on the nature of compounds to be analyzed. In this study,mass spectra in the NICI, positive ion electron impact(PIEI)and positive ion chemical ionization(PICI)modes were recorded and analyzed for neuropsychopharmaceuticals such as benzodiazepines,their acid-hydrolysis product benzophenones and hydantoins.The NICI mode was found not superior in sensitivity than PIEI and PICI modes for 24benzophenones,but is was very useful for identification of halogen groups in drug structures.The mass spectra of 14 benzophenones in the NICI mode showed anions due to halogen and nitro groups, and also very intense molecular anions useful for their sensitive detection by selected ion monitoring.The mass spectra of hydantoins showed a characteristic anions at m/z42, which can be used for screening of the drug group . In addition to the above mass spectral analyses various extraction methods were tested for a number of drugs and poisons such as benzodiazepines, berbiturates,butyrophenones and organophosphate pecticides. As a result, extraction with use of Sep-Pak C18 cartridges was best in view of its rapidness,high recovery and high purity. The above data have appeared or are new in press in some journals

使用负离子化学电离（NICI）质谱法（MS）有一些优点，但这取决于待分析化合物的性质。本研究记录并分析了苯二氮卓类神经精神药物及其酸水解产物二苯甲酮类和氢妥英类药物的NICI、正离子电子冲击（PIEI）和正离子化学电离（PICI）模式的质谱。NICI模式对24种二苯甲酮类药物的敏感性不优于pii和PICI模式，但对药物结构中卤素基团的识别非常有用。在NICI模式下，14种二苯甲酮类化合物的质谱显示出由卤素和硝基引起的阴离子，以及非常强烈的分子阴离子，这有助于通过选择离子监测对其进行敏感检测。其质谱在m/z42处显示一个特征阴离子，可用于药物基团的筛选。除了上述质谱分析外，还测试了各种提取方法对多种药物和毒药的提取，如苯二氮卓类药物、苯比妥酸盐、丁苯酮和有机磷农药。结果表明，Sep-Pak C18萃取筒具有提取速度快、回收率高、纯度高等优点。以上数据已在一些期刊上发表或新发表

项目成果

O. Suzuki; H. Seno; T. Kumazawa: "Rapid isolation of benzodiazepines with Sep-Pak C18 cartridges" J. Forensic Soi.33. 1249-1253 (1988)

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O.Suzuki,;T.Kumazawa,;H.Seno,;H.Hattori: Med.Sci.Law. (1989)

O.Suzuki,;T.Kumazawa,;H.Seno,;H.Hattori：医学科学法。

J.Liu,;O.Suzuki,;T.Kumazawa,;H.Seno: Forensic Sci.Int.(1989)

J.Liu,;O.Suzuki,;T.Kumazawa,;H.Seno：法医学国际 (1989)

H.Seno,;O.Suzuki,;T.Kumazawa,;M.Asano: Z.Rechtsmed.(1989)

H.Seno,;O.Suzuki,;T.Kumazawa,;M.Asano: Z.Rechtsmed.(1989)

O. Suzuki; T. Kumazawa; H. Seno; H. Hattori: "Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of some barbiturates" Med. Sci. Law. (1989)

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