A study of bioactive materials used for filling bone defects

本研究旨在探讨磷酸钙晶体特性对骨替代材料生物活性的影响。由人工合成的磷酸八钙(OCP)和明胶等天然聚合物组成的海绵状复合材料。OCP晶体被机械研磨，尺寸从微米到纳米亚微米，分散在明胶基质中并模压成型。将复合材料在模拟体液中浸泡1周或植入大鼠颅骨临界大小的缺损处8周后进行分析。对浸渍材料的X射线衍射分析表明，随着浸泡时间的延长，羟基磷灰石逐渐被检测到。颅骨脱钙标本的组织学分析表明，在材料部分生物降解的同时，形成了新骨。结果表明，晶体特性可能是影响复合材料生物活性的一个因素。

The present study was designed to investigate the effect of calcium phosphate crystal characteristics on the bioactivity of the bone substitute materials. The spongy composite consists of synthetic octacalcium phosphate (OCP) and natural polymers, such as gelatin, was prepared. OCP crystals, ground mechanically from micrometer to nano-submicro meter scale in size, were dispersed in gelatin matrix and molded. The composite was soaked in simulated body fluid for one week or implanted in rat calvaria critical-sized defect for 8 weeks and then analyzed. X-ray diffraction of the soaked materials showed that hydroxyapatite became detected with the time. Histological analysis of the decalcified specimens of the calvaria indicated that new bone was formed together with the partial biodegradation of the materials. The results suggest that the crystal characteristics may be a factor to affect the bioactivity of the composite.