A study of bioactive materials used for filling bone defects

本研究旨在探讨磷酸钙晶体特性对骨替代材料生物活性的影响。制备了一种由合成磷酸八钙（OCP）和天然高分子（如明胶）组成的海绵状复合材料。OCP晶体，机械研磨从微米到纳米亚微米级的大小，分散在明胶基质和成型。将复合材料浸泡在模拟体液中1周或植入大鼠颅骨临界大小缺损中8周后进行分析。浸泡材料的X射线衍射表明，随着时间的推移，羟基磷灰石变得检测。脱钙标本的组织学分析表明，新骨形成的同时，材料的部分生物降解。结果表明，复合材料的结晶特性可能是影响其生物活性的一个因素。

The present study was designed to investigate the effect of calcium phosphate crystal characteristics on the bioactivity of the bone substitute materials. The spongy composite consists of synthetic octacalcium phosphate (OCP) and natural polymers, such as gelatin, was prepared. OCP crystals, ground mechanically from micrometer to nano-submicro meter scale in size, were dispersed in gelatin matrix and molded. The composite was soaked in simulated body fluid for one week or implanted in rat calvaria critical-sized defect for 8 weeks and then analyzed. X-ray diffraction of the soaked materials showed that hydroxyapatite became detected with the time. Histological analysis of the decalcified specimens of the calvaria indicated that new bone was formed together with the partial biodegradation of the materials. The results suggest that the crystal characteristics may be a factor to affect the bioactivity of the composite.