Developtment of sensitive and rapid methods for analysis of inorganic toxic substances in human specimens

开发灵敏快速的人体样本中无机有毒物质分析方法

• 批准号: 14370153
• 负责人: SUZUKI Osamu
• 金额: $ 7.42万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370153/
• 关键词: Inorganic compound analysis; Ion chromatography; Nitrite anion; Nitrate nion; ESR; Manganese; Molybdenum; Arsenous acid; 原子吸光分析; アジ化物; 鉄; 銅

1)Ion chromatographic analysis of inorganic toxic substancesOn July 25, 1998, the Wakayama Curry Incident, in which 63 people were injured by arsenic and 4 killed, took place. In the same year, many copycat poisoning incidents using other inorganic toxins followed. Therefore, it was essential for us to be prepared to cope with any type of poison analysis including inorganic ones. We introduced a full system of ion chromatography with an autosuppressor, which could cover the analysis of almost every type of inorganic compounds. As the first study using the above instrument, we established a detailed procedure for analysis of nitrite and nitrate anions in human whole blood. There was a serious problem in the assays of nitrite anion in whole blood, because the retention time of nitrite was very close to that of chloride anion which is abundantly present in whole blood. Before application of a sample to ion chromatography, we succeeded in removing the chloride anion using the combination of cartridges of On Guard II AG and On Grand II H. It was found that most of nitrite was oxidized to nitrate within 3 h in whole blood. The recovery rate for nitrite from whole blood was more than 90 %. The linearity was confirmed in the range of 50-100 μg/ml of whole blood. The detection limit of nitrate was sufficientry low. These data were arranged into a scientific paper, which is now in press in an international journal.2)ESR analysis of inorganic substancesIons of manganese(Mn), molybdenum(Mo) and thiocyanate were tried to be measured by ESR after suitable pretreatments. Mn ion shows paramagnetic property and was thus measurable by ESR only after asking a sample. Mo ion reacted with diethyldithiocarbamate and the complex could be measured by ESR with high sensitivity after reduction by oxalic acid. Thiocyanate could be also measured after complex formation. These results were published in analytical journals.

1)无机有毒物质的离子色谱分析1998年7月25日，发生了63人被砷炸伤、4人死亡的和歌山咖喱事件。同年，许多模仿使用其他无机毒素的中毒事件接踵而至。因此，我们必须准备好科普任何类型的毒物分析，包括无机毒物分析。我们介绍了一个完整的系统，离子色谱与自动抑制器，它可以涵盖几乎所有类型的无机化合物的分析。作为使用上述仪器的第一项研究，我们建立了一个详细的程序分析人体全血中的亚硝酸盐和硝酸盐阴离子。全血中亚硝酸根阴离子的保留时间与全血中大量存在的氯阴离子的保留时间非常接近，这给全血中亚硝酸根阴离子的测定带来了严重的问题。在将样品应用于离子色谱法之前，我们成功地使用On Guard II AG和On Grand II H柱的组合去除了氯离子。结果表明，全血中绝大部分亚硝酸盐在3 h内被氧化为硝酸盐。全血中亚硝酸盐的回收率大于90%。在50-100 μg/ml全血范围内确认了线性。硝酸盐的检出限低得令人发指。这些数据整理成一篇科学论文，现已在国际期刊上出版。2）无机物的ESR分析Mn离子显示出顺磁性，因此仅在询问样品后才可通过ESR测量。钼离子与二乙基二硫代氨基甲酸盐反应，经草酸还原后，可用ESR高灵敏度测定。硫氰酸盐也可以在复合物形成后测量。这些结果发表在分析期刊上。

项目成果

K.Watanabe: "Stability of alkyl alcohols as decomposition products of alkyl nitrites in human whole blood"Jpn.J.Forensic Toxicol.. 21・3. 256-261 (2003)

K.Watanabe：“作为亚硝酸烷基酯分解产物的烷基醇在人全血中的稳定性”Jpn.J.Forensic Toxicol.. 21・3 (2003)。

南方かよ子: "ビタミンC欠ラットの電子スピン共鳴法を用いたマンガンの定量"Biomedical Research on Trace Elements. 13・4. 320-321 (2002)

Kayoko Minakata：“利用电子自旋共振对缺乏维生素 C 的大鼠进行锰定量”《微量元素生物医学研究》13・4（2002 年）。

Sensitive analysis of alkyl alcohols as decomposition products of alkyl nitraites in human whole bood and urine by headspace capillary GC with cryogenic oven trapping

通过顶空毛细管气相色谱和低温烘箱捕集技术对人体全血和尿液中亚硝酸烷基酯分解产物烷基醇进行灵敏分析

• 发表时间: 2003
• 期刊: J.Chromatogr.Sci. 41.1
• 作者: K.Watanabe-Suzuki

Effects of paraquat on essential antioxidant elements in osteogenic disorder Shinogi rat

百草枯对成骨性疾病 Shinogi 大鼠必需抗氧化元素的影响

• 发表时间: 2002
• 期刊: J.Toxicol.Environ.Health, PartA 65
• 作者: K.Minakata

Drugs and poisons in humans : a handbook of practical analysis

• 发表时间: 2005
• 作者: O. Suzuki;Kanako Watanabe