DEVELOPMENT OF NEW METHODS FOR ANALYSIS OF ILLICIT DRUGS : COMBINATION OF UPLC AND TOF-MS

在已经引进的飞行时间(TOF)质谱仪的基础上，连接了一台新的超功率液相色谱仪(UPLC)，该仪器由目前的赠款购买，用于创建一个强大的药物和毒物分析系统。为了实现这种在线连接，出现了各种问题，这些问题已经被克服。作为第一次分析实验，我们使用了鹅膏菌毒素和鬼臼毒素，这两种典型的蘑菇毒素。在UPLC-单级TOF-MS模式下对这些毒素进行了鉴定和定量。作为第二组目标化合物，我们在单级TOF-MS模式下分析了六种色胺类设计药物。每种化合物都有质子化的分子同位素峰，它们的模式可用于每种化合物的鉴定。此外，即使在串联四极杆-TOF-MS模式下，也可以获得可用于每种设计药物的特定定量的良好产品离子，这取决于碰撞诱导解离的电压。

To an already introduced time-of-flight (TOF)-mass spectrometer, a new ultra-power liquid chromatography (UPLC), which was bought by the present grant, has been connected to create a powerful system for analysis of drugs and poisons. To realize such an on-line connection, various problems appeared ; these problems have been overcome. As the first analytical experiments, we used amatoxins and phalloidin, typical mushroom toxins. These toxins were found to be identified and quantitated in the UPLC-single-stage TOF-MS mode. As the second group of target compounds, we analyzed six tryptamine-type designer drugs in the single-stage TOF-MS mode. Each compound also gave protonated molecular isotopic peaks and their pattern was usable for identification of each compound. Moreover, even in the tandem quadrupole-TOF-MS mode, good product ions usable for specific quantitation of each designer drug could be obtained, depending on voltages for collision induced dissociation.