DEVELOPMENT OF NEW METHODS FOR ANALYSIS OF ILLICIT DRUGS : COMBINATION OF UPLC AND TOF-MS

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To an already introduced time-of-flight (TOF)-mass spectrometer, a new ultra-power liquid chromatography (UPLC), which was bought by the present grant, has been connected to create a powerful system for analysis of drugs and poisons. To realize such an on-line connection, various problems appeared ; these problems have been overcome. As the first analytical experiments, we used amatoxins and phalloidin, typical mushroom toxins. These toxins were found to be identified and quantitated in the UPLC-single-stage TOF-MS mode. As the second group of target compounds, we analyzed six tryptamine-type designer drugs in the single-stage TOF-MS mode. Each compound also gave protonated molecular isotopic peaks and their pattern was usable for identification of each compound. Moreover, even in the tandem quadrupole-TOF-MS mode, good product ions usable for specific quantitation of each designer drug could be obtained, depending on voltages for collision induced dissociation.