Analysis of leukemia related oncogene and mRNA using double staining fo nucleic acid and protein

核酸和蛋白质双染色分析白血病相关癌基因和 mRNA

• 批准号: 62570436
• 负责人: MAKINO Shun-ichi
• 金额: $ 1.41万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570436/
• 关键词: Neuroblastoma; Leukemia; フローサイトメトリー; 小児白血症; high resolution; DNA分析

With flow-cyto metry, it would be able to elucidate the cell cycle by measuring and analysing the DNA volume. We studied the flow-cyto metry as well as chromosome analysis and immuno cyto chemical staning.(1) Leukemia cell have two lineages, i.e., one with G_1 and SG_2M and other only with SG_2M. conservative methodology failed to visualize the latter lineage. The fact would express the poly clonal character of the cells. Similarly, neuroblastoma cell revealed the more than two cell lineages. There findings showed the traverse distrubance on S-phase, expressing the presense of silent S which is noted regarding to the clonal evolution and oncogene amplification.(2) Flow-cyto metry is very useful for the detection of chromosmal aberration of neuriblastoma. Cases with hyperdiploidity and near diploidity were considered as good prognosis, but cases with near diploidity, hypotetraploidity were as poor prognosis. N-myc amplification was found only among the latter cases.(3) In addition, immunohistochemical staining utilizing neuro specific enolase was done on neuroblastoma cells. As the staining was remarkeably specific, it is now on trial whether the sataing of the neuroblastoma cells can be applied with FCM and cell biology.

用流式细胞仪，可以通过测量和分析DNA体积来阐明细胞周期。我们对流式细胞术、染色体分析和免疫细胞化学染色进行了研究。(1)白血病细胞有两个谱系，即，一个具有G_1和SG_2M，另一个仅具有SG_2M。保守的方法论未能将后一种谱系形象化。这一事实将表达细胞的多克隆特性。同样，神经母细胞瘤细胞也显示出两种以上的细胞谱系。结果表明，S期的横向紊乱，表现为沉默S的存在，这与克隆进化和癌基因扩增有关。(2)流式细胞术是检测神经母细胞瘤染色体畸变的有效方法。超二倍体和近二倍体预后好，近二倍体和低四倍体预后差。N-myc扩增仅见于后者。(3)此外，利用神经特异性烯醇化酶对神经母细胞瘤细胞进行免疫组织化学染色。由于该染色方法特异性强，因此，FCM和细胞生物学方法能否应用于神经母细胞瘤细胞的定位尚待进一步研究。

项目成果

鞭煕 他: 小児がん.

Fuchihiro 等人：儿童癌症。

Hiromu Muchi et al: Cancer.

Hiromu Muchi 等人：癌症。

H.Hayashi,;H.Muchi,et al: Cancer. 63. 126-132 (1989)

H.Hayashi，；H.Muchi，等：癌症。

Hiromu,Muchi; Hiroko,Ijima; Hideo,Shimoizumi,: "Detection of Neuroblastoma cells in the bone marrow with immunohistochemical staining of neurone specific enolase" Pediatric Hematology and Oncology. 6. 63-66 (1989)

弘、穆奇；

H.Hayashi;H.Muchi;et al.: Cancer. 63. 126-132 (1989)

H.Hayashi；H.Muchi；等：癌症。