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血友病患者における活動性AIDSウイルス保有者のモノクローナル抗体による同定

使用单克隆抗体鉴定血友病患者中的活动性艾滋病病毒携带者

基本信息

批准号：
62570556
负责人：
佐川公矯
金额：
$ 1.15万
依托单位：
Kurume University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570556/
关键词：
AIDS AIDSウイルス HIV HIV抗原 p24 gag遺伝子産物 AIDSの発病予知抗HIVモノクローナル抗体

项目摘要

1.AIDSウイルス(HIV)保有者のAIDS発病の予知に有用なマーカーを得ることを目的として, 血友病患者の末梢血リンパ球内に出現するHIV抗原(p24)を検索し, 他のマーカーとの比較検討を試みた.2.血友病患者の末梢血より単核球を分離し, PHA,IL-2,polybreneと共に10日間培養した. この培養単核球の塗沫標本を固定し, 抗HIVP24モノクローナル抗体(VAK5)を使用した間接蛍光抗体法によって, リンパ球内に発現するHIV抗原(p24)を同定した. 同時に, 患者血清中の抗HIV抗体を, Serodia-HIV法とELISA法で測定した. また, 末梢血リンパ球の膜抗原を各種モノクローナル抗体とflow cytometryを用いて解析した.3.血清中の抗HIV抗体は, 血友病36名中25名(69%)が陽性で, 内訳は血友病A20名中11名(55%)が陽性, 血友病B14中13名(93%)が陽性, von Willebrand病2名中1名(50%)が陽性であった. リンパ球内のHIV抗原は血友病36名中7名(19%)が陽性であった. 内訳は, 血友病A20名中5名(25%)が陽性, 血友病B14名中2名(14%)が陽性であった. なお, HIV抗原陽性者はすべて抗HIV抗体陽性であった. 抗HIV抗体陽性者のうちHIV抗原陽性者は28%であった.4.抗HIV抗体とHIV抗原を指標として血友病患者を区分すると, A群(抗体陰性・抗原陰性)は12名, B群(抗体陽性・抗原陰性)は17名, C群(抗体陽性・抗原陽性)は7名であった. この圧分とリンパ球の膜抗原の解析結果を統合すると, CD4/CD8比の平均がA群は1.35, B群は0.66, C群は0.59であり, A群と比べB群とC群は有意に低く, HIV保有者の細胞性免疫能の低下が確認された. また, C群7名中2名はARC状態であり, 他の1名はAIDSを発症して死亡した事実から, HIV抗原の検出は発病を予知するマーカーとして有用なものであることが示唆された.

1. The owner of AIDS disease (HIV) is not aware of the disease, but it is useful and effective. HIV antigen (p24) appears in the peripheral blood globulins of patients with hemophilia. He also performed a comparative study on the subject. 2. The peripheral blood of patients with hemophilia was isolated and cultured for 10 days with PHA, IL-2, polybrene and polybrene. The cultured mononuclear pellet smear specimen was fixed, and the anti-HIVP24 antibody (VAK5) was used using the indirect photoantibody method. The HIV antigen (p24) present in the human body was determined at the same time. At the same time, the anti-HIV antibodies in the patient's serum were determined by Serodia-HIV method and ELISA method. Peripheral blood glomus membrane antigen and various neutrophil antibodies were analyzed by flow cytometry. 3. Anti-HIV antibodies in serum, 25 out of 36 (69%) patients with hemophilia were positive, 11 out of 20 (55%) patients were positive for hemophilia A, 13 (93%) out of 20 patients (93%) were positive for hemophilia B14, and 1 out of 2 (50%) patients were positive for von Willebrand disease. HIV antigen in the intraglobule was found to be positive in 7 out of 36 (19%) patients with hemophilia. It was found to be positive in 5 out of 20 (25%) patients with hemophilia A, and in 2 out of 14 (14%) patients with hemophilia B. Na, HIV antigen positive person, anti-HIV antibody positive person, and anti-HIV antibody positive person. Anti-HIV antibody-positive people and HIV antigen-positive people accounted for 28%. 4. Anti-HIV antibodies and HIV antigen indicators differentiated hemophilia patients, 12 people were in group A (antibody negative and antigen negative), and 17 people were in group B (antibody positive and antigen negative). Group C (antibody-positive, antigen-positive) 7 people. Analytical results of the analysis of the membrane antigen of the group A and the average CD4/CD8 ratio were 1.35 for group A and 0.66 for group B, Group C is 0.59, group A is intentionally lower than group B and group C, and the HIV carrier's cellular immunity is low and confirmed. 2 out of 7 people in group C are in ARC status, His name is AIDS and he died of AIDS syndrome, HIV antigen の検出は発 Disease するマーカーとして is useful and なものであることがshows instigation された.