Cytodynamic Evaluation on the Relation Ship Between the mode of intracellular DNA content and the Prognosis of Esophageal Cancer Patients

为了阐明影响患者预后的食管癌生物学特征，采用流式细胞术（FCM）检测细胞内DNA含量。根据第一峰曲线的形状和第二峰曲线的存在，将112例手术切除的石蜡包埋标本的DNA倍体模式分为4类。A型（65例）：对称单峰曲线。B型（21例）：单峰曲线不对称。C型（12例）：第二个小峰曲线出现在第一个峰曲线的右侧。D型（14例）：有两个明显的峰曲线。由于存在异常的茎系，B型、C型和D型大致属于非整倍体型。这些DNA倍体模式与组织学类型密切相关。然而，DNA多态性模式与临床分期（如癌症侵袭程度和淋巴结转移）的关系没有显著差异。没有发现肿瘤侵袭时dna含量增加的趋势。人们认为，自从癌症发生以来，癌细胞的dna含量是恒定的。整倍体型患者4年生存率为65.5%，非整倍体型患者4年生存率为15.7%，前者与后者的生存率有显著差异。DNA倍体类型被认为是独立于其他病理因素的食管癌患者预后的重要指标之一。

In order to elucidate biological features of esophageal carcinoma influencing the prognosis of patients, intracellular DNA content was determined using a flowcytometry (FCM) apparatus.DNA ploidy patterns in 112 parffin-embedded specimens removed at surgery were classified into 4 categories according to the shape of the first peak curve and the presence of second peak curve.Type A (65 cases): with symmetric single peak curve. (euploidy type) Type B (21 cases): with asymmetric single peak curve. Type C (12 cases): with the second small peak curve appearing on the right side of the firsat peak curve. Type D (14 cases): with two obvious peak curves.Types B, C and D were grossly included in aneuploidy type, because of the presence of abnormal stem line.These DNA ploidy patterns were well correlated with histologic types. There were, however no signifficant differences in the relationship of DNA polidy patterns and clinical stages such as the degree of cancerous invasion and nodal metastases.The tendency of increasing dna content acompanyaing to tumor invasion was not found. It was considered that the dna content of a cancer cell was constant since cancer had occurred. Four-year survival rate of the patients with euploidy pattern was 65.5% and that with aneuploidy pattern was 15.7%, indicating a significant difference in survival between patients with the former pattern and those with the latter.The type of DNA ploidy was considered as one of the important prognostic indicators in patients with esophageal carcinoma independent of any other pathologic factors.