Cytodynamic Evaluation on the Relation Ship Between the mode of intracellular DNA content and the Prognosis of Esophageal Cancer Patients

细胞内DNA含量模式与食管癌患者预后关系的细胞动力学评价

• 批准号: 62570601
• 负责人: TANAKA Otsuo
• 金额: $ 1.34万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570601/
• 关键词: Esophageal carcinoma; Flow-cytometry; DNA分析; フローサイトメトリー; 食道癌の悪性度; DNAヒストグラムパターン; euploidy; aneuploidy; DNA; Brdu二重染色法

In order to elucidate biological features of esophageal carcinoma influencing the prognosis of patients, intracellular DNA content was determined using a flowcytometry (FCM) apparatus.DNA ploidy patterns in 112 parffin-embedded specimens removed at surgery were classified into 4 categories according to the shape of the first peak curve and the presence of second peak curve.Type A (65 cases): with symmetric single peak curve. (euploidy type) Type B (21 cases): with asymmetric single peak curve. Type C (12 cases): with the second small peak curve appearing on the right side of the firsat peak curve. Type D (14 cases): with two obvious peak curves.Types B, C and D were grossly included in aneuploidy type, because of the presence of abnormal stem line.These DNA ploidy patterns were well correlated with histologic types. There were, however no signifficant differences in the relationship of DNA polidy patterns and clinical stages such as the degree of cancerous invasion and nodal metastases.The tendency of increasing dna content acompanyaing to tumor invasion was not found. It was considered that the dna content of a cancer cell was constant since cancer had occurred. Four-year survival rate of the patients with euploidy pattern was 65.5% and that with aneuploidy pattern was 15.7%, indicating a significant difference in survival between patients with the former pattern and those with the latter.The type of DNA ploidy was considered as one of the important prognostic indicators in patients with esophageal carcinoma independent of any other pathologic factors.

为探讨食管癌的生物学特性对患者预后的影响，采用流式细胞仪测定细胞内DNA含量。根据第一峰曲线的形状和第二峰曲线的存在，将112例手术切除的石蜡包埋标本的DNA倍体类型分为4类。A型65例，单峰对称。(整倍体型)B型21例，单峰曲线不对称。C型12例，第二小峰曲线位于Firsat峰曲线右侧。D型14例，有两个明显的峰值曲线，B、C、D型主要为异倍体型，因存在异常干系，DNA倍体类型与组织学类型密切相关。但DNA倍体类型与肿瘤侵袭程度、淋巴结转移等临床分期的关系无明显差异，未发现DNA含量随肿瘤侵袭程度的增加而增加的趋势。人们认为，自从癌症发生以来，癌细胞的DNA含量是恒定的。整倍体和非整倍体患者的四年生存率分别为65.5%和15.7%，差异有统计学意义，DNA倍体类型可作为食管癌患者独立于其他病理因素的重要预后指标之一。

项目成果

Masaki Hasegawa: "Cytodynamic Evaluation on the Relationship Between The Mode of Intracellular DNA Content and the Prognosis of Esophageal Cancer Patients" Journal of Japan Surgical Society.

长谷川正树：“细胞内DNA含量模式与食道癌患者预后关系的细胞动力学评价”日本外科学会杂志。

長谷川正樹: 日外会誌.

长谷川正树：日本和外国协会杂志。