Omics, Bioinformatics and Flow Cytometry Core

组学、生物信息学和流式细胞术核心

• 批准号: 10630580
• 负责人: MICHAEL R GARRETT
• 金额: $ 22.41万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-15 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630580
• 关键词: African American population; Apoptosis; Bioinformatics; Biological; Biological Assay; Biological Process; Cardiorenal syndrome; Cell Cycle; Cell Nucleus; Cell Proliferation; Cell Separation; Cells; Centers of Research Excellence; Clinical; Complement; Consultations; Country; Data Set; Disease; Enhancement Technology; Ensure; Equipment; Faculty; Faculty Workshop; Flow Cytometry; Funding; Gene Expression; Generations; Genetic; Genomics; Goals; Health; High Prevalence; Historically Black Colleges and Universities; Immune; Infrastructure; Institution; Instruction; Maintenance; Mass Spectrum Analysis; Metabolic Diseases; Methodology; Mississippi; Molecular; Molecular Analysis; Molecular Biology; Morbidity - disease rate; Names; Nucleic Acids; Obesity; Phase; Phenotype; Pilot Projects; Population; Proteomics; Quality Control; Research; Research Personnel; Research Project Grants; Resources; Scientific Advances and Accomplishments; Scientist; Services; Small RNA; Sorting; Students; Supervision; Systems Biology; Technical Expertise; Techniques; Technology; Training and Education; United States; Work; XCL1 gene; cell type; complex data; epigenome; genome sequencing; genomic data; glucose uptake; improved; insight; instrument; instrumentation; meetings; mortality; multidisciplinary; multiple omics; next generation sequencing; programs; release of sequestered calcium ion into cytoplasm; service delivery; single-cell RNA sequencing; training opportunity; transcriptome; transcriptome sequencing; transcriptomics; undergraduate student; whole genome

CORE C – OMICS, BIOINFORMATICS AND FLOW CYTOMETRY CORE PROJECT SUMMARY/ABSTRACT The goal of the Cardiorenal and Metabolic Disease Research Center (CMDRC) COBRE is to bring together a multidisciplinary group of basic, clinical, and population scientists to work synergistically on the theme of cardiorenal and metabolic diseases. During Phases 1 and 2, the CMDRC - Core C provided COBRE investigators and external researchers access to technical expertise and equipment to perform a wide variety of molecular, genetic, and next generation sequencing studies. During Phases 1 and 2, Core C also made major advances toward ensuring sustainability of its services. During Phase 3, the newly named Core C - Omics, Bioinformatics and Flow Cytometry will extend its services and offer cutting-edge cellular and molecular analysis through building on existing infrastructure supported in Phases 1 and 2 (Genomics and Mass Spectrometry) to offer new cutting-edge omics methodologies as well as offer new capabilities through recently acquired state-of-the-art cell sorting and flow cytometry equipment. The Omics and Bioinformatics Sub-Core and Cell Sorting/Flow Cytometry Sub-Core will allow CMDRC investigators comprehensive molecular or systems biology approaches to their research questions through detailed insight into cell-type specific function, generation of “omics” datasets, and ultimately integration with detailed phenotype information to provide greater depth and insight into biological processes associated with obesity, cardiorenal, and metabolic diseases. Core C has four aims: (1) to provide CMDRC investigators access to technical expertise and cutting-edge omics services, including next generation sequencing technologies (single cell RNA sequencing, spatial transcriptomics) and facilitate proteomic technologies by leveraging existing IDeA funded infrastructure (offer and coordinate proteomics services and through IDeA National Resource for Quantitative Proteomics program); (2) to provide CMDRC investigators access to cutting-edge cell sorting and flow cytometry expertise and services; (3) to provide education and training opportunities for students, trainees, and faculty in omics technologies and flow cytometry; and (4) to provide continuous improvement in existing assays and services and to enhance technological capabilities through acquisition of new cutting- edge instrumentation that will assist in sustainability of the Core. Therefore, it is the objective of the CMDRC - Core C to aid investigators in advancing scientific discovery in obesity, cardiorenal, and metabolic diseases through application of state-of-the-art cell sorting, flow cytometry, and omics technologies to generate greater insights into these diseases. Aside from CMDRC investigators, continued funding of the COBRE during Phase 3 will have a significant impact on providing external researchers (at other Mississippi undergraduate institutions and Historically Black Colleges and Universities (HBCUs)) access to equipment and expertise that would otherwise not be available in our region of the country.

核心 C – 组学、生物信息学和流式细胞术核心 项目概要/摘要 心肾和代谢疾病研究中心 (CMDRC) COBRE 的目标是将 由基础、临床和人口科学家组成的多学科小组，就以下主题协同工作 心肾和代谢疾病。在第 1 阶段和第 2 阶段，CMDRC - Core C 提供了 COBRE 研究人员和外部研究人员可以获得技术专业知识和设备来执行各种任务 分子、遗传和下一代测序研究。在第一阶段和第二阶段，核心 C 还做出了 确保其服务可持续性的重大进展。在第 3 阶段，新命名的 Core C - 组学、生物信息学和流式细胞术将扩展其服务并提供尖端的细胞和 通过建立第一阶段和第二阶段支持的现有基础设施（基因组学和 质谱）提供新的尖端组学方法，并通过以下方式提供新功能 最近购买了最先进的细胞分选和流式细胞术设备。组学和生物信息学 子核心和细胞分选/流式细胞术子核心将使 CMDRC 研究人员能够全面了解 通过对细胞类型的详细了解，分子或系统生物学方法来解决他们的研究问题 特定功能，生成“组学”数据集，并最终与详细表型整合 信息，以更深入地了解与肥胖、心肾、 和代谢性疾病。核心 C 有四个目标：(1) 为 CMDRC 调查人员提供技术支持 专业知识和尖端组学服务，包括下一代测序技术（单细胞 RNA 测序、空间转录组学）并利用现有 IDeA 促进蛋白质组学技术 资助的基础设施（提供和协调蛋白质组学服务，并通过 IDeA 国家资源 定量蛋白质组学计划）； (2) 为 CMDRC 研究人员提供尖端细胞分选技术 以及流式细胞术专业知识和服务； (3)为学生提供教育和培训机会， 组学技术和流式细胞术的学员和教员； (4) 持续改进 现有的检测和服务，并通过收购新的切割技术来增强技术能力 边缘仪器将有助于核心的可持续性。因此，这是 CMDRC 的目标 - 核心 C 帮助研究人员推进肥胖、心肾和代谢疾病的科学发现 通过应用最先进的细胞分选、流式细胞术和组学技术来产生更多 对这些疾病的深入了解。除了 CMDRC 调查员之外，COBRE 在阶段期间继续提供资金 3将对提供外部研究人员（密西西比州其他本科生）产生重大影响 机构和传统黑人学院和大学 (HBCU)）获得设备和专业知识， 否则将无法在我们国家的地区使用。