Omics, Bioinformatics and Flow Cytometry Core

组学、生物信息学和流式细胞术核心

• 批准号: 10630580
• 负责人: MICHAEL R GARRETT
• 金额: $ 22.41万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-15 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630580
• 关键词: African American population; Apoptosis; Bioinformatics; Biological; Biological Assay; Biological Process; Cardiorenal syndrome; Cell Cycle; Cell Nucleus; Cell Proliferation; Cell Separation; Cells; Centers of Research Excellence; Clinical; Complement; Consultations; Country; Data Set; Disease; Enhancement Technology; Ensure; Equipment; Faculty; Faculty Workshop; Flow Cytometry; Funding; Gene Expression; Generations; Genetic; Genomics; Goals; Health; High Prevalence; Historically Black Colleges and Universities; Immune; Infrastructure; Institution; Instruction; Maintenance; Mass Spectrum Analysis; Metabolic Diseases; Methodology; Mississippi; Molecular; Molecular Analysis; Molecular Biology; Morbidity - disease rate; Names; Nucleic Acids; Obesity; Phase; Phenotype; Pilot Projects; Population; Proteomics; Quality Control; Research; Research Personnel; Research Project Grants; Resources; Scientific Advances and Accomplishments; Scientist; Services; Small RNA; Sorting; Students; Supervision; Systems Biology; Technical Expertise; Techniques; Technology; Training and Education; United States; Work; XCL1 gene; cell type; complex data; epigenome; genome sequencing; genomic data; glucose uptake; improved; insight; instrument; instrumentation; meetings; mortality; multidisciplinary; multiple omics; next generation sequencing; programs; release of sequestered calcium ion into cytoplasm; service delivery; single-cell RNA sequencing; training opportunity; transcriptome; transcriptome sequencing; transcriptomics; undergraduate student; whole genome

CORE C – OMICS, BIOINFORMATICS AND FLOW CYTOMETRY CORE PROJECT SUMMARY/ABSTRACT The goal of the Cardiorenal and Metabolic Disease Research Center (CMDRC) COBRE is to bring together a multidisciplinary group of basic, clinical, and population scientists to work synergistically on the theme of cardiorenal and metabolic diseases. During Phases 1 and 2, the CMDRC - Core C provided COBRE investigators and external researchers access to technical expertise and equipment to perform a wide variety of molecular, genetic, and next generation sequencing studies. During Phases 1 and 2, Core C also made major advances toward ensuring sustainability of its services. During Phase 3, the newly named Core C - Omics, Bioinformatics and Flow Cytometry will extend its services and offer cutting-edge cellular and molecular analysis through building on existing infrastructure supported in Phases 1 and 2 (Genomics and Mass Spectrometry) to offer new cutting-edge omics methodologies as well as offer new capabilities through recently acquired state-of-the-art cell sorting and flow cytometry equipment. The Omics and Bioinformatics Sub-Core and Cell Sorting/Flow Cytometry Sub-Core will allow CMDRC investigators comprehensive molecular or systems biology approaches to their research questions through detailed insight into cell-type specific function, generation of “omics” datasets, and ultimately integration with detailed phenotype information to provide greater depth and insight into biological processes associated with obesity, cardiorenal, and metabolic diseases. Core C has four aims: (1) to provide CMDRC investigators access to technical expertise and cutting-edge omics services, including next generation sequencing technologies (single cell RNA sequencing, spatial transcriptomics) and facilitate proteomic technologies by leveraging existing IDeA funded infrastructure (offer and coordinate proteomics services and through IDeA National Resource for Quantitative Proteomics program); (2) to provide CMDRC investigators access to cutting-edge cell sorting and flow cytometry expertise and services; (3) to provide education and training opportunities for students, trainees, and faculty in omics technologies and flow cytometry; and (4) to provide continuous improvement in existing assays and services and to enhance technological capabilities through acquisition of new cutting- edge instrumentation that will assist in sustainability of the Core. Therefore, it is the objective of the CMDRC - Core C to aid investigators in advancing scientific discovery in obesity, cardiorenal, and metabolic diseases through application of state-of-the-art cell sorting, flow cytometry, and omics technologies to generate greater insights into these diseases. Aside from CMDRC investigators, continued funding of the COBRE during Phase 3 will have a significant impact on providing external researchers (at other Mississippi undergraduate institutions and Historically Black Colleges and Universities (HBCUs)) access to equipment and expertise that would otherwise not be available in our region of the country.

核心组学、生物信息学和流式细胞术核心 项目总结/摘要 肾脏和代谢疾病研究中心（CMDRC）COBRE的目标是汇集 一个由基础、临床和人口科学家组成的多学科小组，协同工作， 心肾和代谢疾病。在第1和第2阶段，CMDRC -核心C提供了COBRE 调查人员和外部研究人员获得技术专长和设备， 分子、基因和下一代测序研究的重要性。在第1阶段和第2阶段，核心C还 在确保其服务的可持续性方面取得了重大进展。在第三阶段，新命名的核心C - 组学，生物信息学和流式细胞术将扩展其服务，并提供尖端的细胞和 通过在第1阶段和第2阶段支持的现有基础设施上进行分子分析（基因组学和 质谱）提供新的尖端组学方法，并通过 最近获得了最先进的细胞分选和流式细胞仪设备。组学与生物信息学 子核心和细胞分选/流式细胞术子核心将使CMDRC研究者能够全面了解 分子或系统生物学通过对细胞类型的详细了解来解决研究问题 特定功能，“组学”数据集的生成，以及最终与详细表型的整合 信息，以提供更深入的了解与肥胖，心肾， 和代谢性疾病。核心C有四个目标：（1）为CMDRC调查人员提供技术 专业知识和尖端的组学服务，包括下一代测序技术（单细胞 RNA测序、空间转录组学），并通过利用现有的IDEA 资助的基础设施（提供和协调蛋白质组学服务，并通过IDEA国家资源， 定量蛋白质组学计划）;（2）为CMDRC研究人员提供尖端细胞分选技术 和流式细胞仪的专业知识和服务;（3）为学生提供教育和培训机会， 学员和教员在组学技术和流式细胞术;及（4）提供持续改进， 现有的分析和服务，并通过收购新的切割技术来提高技术能力， 边缘仪器，这将有助于核心的可持续性。因此，CMDRC的目标是 - 核心C帮助研究人员推进肥胖，心肾和代谢疾病的科学发现 通过应用最先进的细胞分选、流式细胞术和组学技术， 对这些疾病的认识。除了CMDRC调查员之外，COBRE在阶段期间的持续资助 3将对提供外部研究人员产生重大影响（在其他密西西比本科 机构和历史上的黑人学院和大学（HBCU））获得设备和专业知识， 否则在我国的这一地区将无法获得。