Coronavirus Challenge Core

项目总结-动物攻毒核心B 动物挑战核心B将为四种不同的人类发展和提供关键的病毒储备。 和具有大流行潜力的动物冠状病毒（CoV）。这些病毒属于 Sarbecovirus和Merbecovirus的亚属，并将由核心用于攻毒研究 在小鼠和叙利亚仓鼠中，评估疫苗对不同 β冠状病毒。核心B产生的病毒储备液也将用于病毒中和 在小鼠和仓鼠中进行疫苗攻击前或免疫原性试验后进行试验。的 核心B的具体职能将包括：（a）产生四种不同的经验证的库存， β冠状病毒，包括SARS-CoV-1、SARS-CoV-2、SHC-014和穿山甲/MP 789/2019; (b)开发蝙蝠冠状病毒的啮齿动物模型;（c）促进并开展关键的 在小鼠和仓鼠中进行免疫原性研究，以定量B和T细胞对疫苗接种的应答 与项目1-3中产生的不同候选疫苗;（d）进行病毒攻击 在用候选疫苗免疫的小鼠和仓鼠中对人和蝙蝠CoV进行的研究， 诱导对多种β冠状病毒广泛反应性B-和T-细胞应答。的结果 核心B将由核心A中的数据管理团队用于识别免疫相关性 对引起大流行的乙型冠状病毒的交叉保护。这些函数将使 PPG组确定候选疫苗是否诱导广泛的保护性免疫 对抗人类和动物的冠状病毒这个核心B，由弗里曼和布恩博士领导，将满足需求 通过获取和产生挑战病毒，开发动物模型， 在已建立的啮齿动物模型中进行免疫原性和疫苗攻毒研究， β冠状病毒。这些功能为所有研究项目提供关键的基础设施支持， 的PPG。

Project Summary – Animal Challenge Core B The Animal Challenge Core B will develop and provide key virus stocks for four different human and animal coronaviruses (CoVs) that have pandemic potential. These viruses belong to the subgenera of Sarbecovirus and Merbecovirus and will be used by the Core for challenge studies in mice and Syrian hamsters to evaluate cross-protective efficacy of vaccines against diverse betacoronaviruses. The virus stocks, generated by Core B, also will be used in virus neutralization assays prior to challenge or after immunogenicity testing of vaccines in mice and hamsters. The specific functions of Core B will include: (a) generating validated stocks of four different betacoronaviruses including SARS-CoV-1, SARS-CoV-2, SHC-014 and pangolin/MP789/2019; (b) developing rodent models for bat coronaviruses; (c) Contributing to and performing pivotal immunogenicity studies in mice and hamsters to quantify the B and T cell response to vaccination with different vaccine candidates generated in Projects 1-3; (d) Performing virus challenge studies with human and bat CoV in mice and hamsters immunized with candidate vaccines that induce broadly reactive B- and T-cell responses to diverse betacoronaviruses. The results from Core B will be used by the data management team in Core A to identify correlates of immune cross-protection against betacoronaviruses of pandemic concern. These functons will enable the PPG group to determine whether a candidate vaccine induces broadly protective immunity against human and animal CoV. This Core B, led by Drs. Frieman and Boon, will meet the needs of the PPG by acquiring and generating challenge viruses, developing animal models and performing immunogenicity and vaccine challenges studies in established rodent models of betacoronaviruses. These functions provide key infrastructure support for all research projects of the PPG.