Isolation and purification of gastric inhibitors from canine gastrointestinal mucosa

犬胃肠粘膜胃抑制剂的分离纯化

• 批准号: 63044141
• 负责人: GOTO Yoshiaki
• 金额: $ 2.37万
• 依托单位: Tokushima Bunri University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Overseas Scientific Survey.
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63044141/
• 关键词: Stomach; Gastric Secretion; Fundic Inhibitor

Having shown that excision of the oxyntic mucosa of the dog stomach removes a powerful inhibitor, we attempted preliminary isolation of the inhibitor from canine fundic mucosa. The inhibitor was extracted from boiled, homogenized canine gastric mucosa in 2 % cold trifluoroacetic acid (TFA), centrifuged, filtrated and lyophilized. The lyophilized extract was dissolved in water and first purification on Sephadex G10. The inhibitor was found in the salt peak fractions on elution with 0.1 % TFA. These fractions were pooled, loaded onto a reverse-phase C18 column and purified by HPLC. Inhibitory activity of the fractions was tested on pentagastrin-stimulated acid secretion in the rat. The inhibitor is not retained on the HPLC and after ether extraction under both acidic and alkaline condition, the inhibitor was concentrated in aqueous phase of an aqueous/lipid solvent system. This suggests that the inhibitor is a charged, zwitterionic substance, possible a small protein

在证明了切除狗胃的含氧酸粘膜可以去除一种强大的抑制物后，我们尝试从犬的胃底粘膜中初步分离出这种抑制物。从2%冷三氟乙酸(TFA)中煮沸、匀浆的犬胃粘膜中提取抑制物，离心、过滤、冷冻干燥。冻干提取液溶于水，Sephadex G10柱层析纯化。用0.1%TFA洗脱时，在盐峰部分发现了该抑制剂。这些组分混合在一起，加载到反相C18柱上，并通过高效液相色谱法进行纯化。测定其对五肽胃泌素刺激的大鼠胃酸分泌的抑制作用。缓蚀剂在高效液相色谱上没有保留，在酸性和碱性条件下乙醚萃取后，缓蚀剂被浓缩在水/脂类溶剂体系的水相中。这表明该抑制剂是一种带电的两性离子物质，可能是一种小的蛋白质。

项目成果

Goto, Y., Ohno, H. and Tache, Y.: Possible involvement of central mechanisms in antiulcer and antisecretory actions of prostaglandins. In ULCER DISEASE; New aspects of pathogenesis and pharmacology.CRC Press (Florida, U.S.A.), 229-234 (1989)

Goto, Y.、Ohno, H. 和 Tache, Y.：中枢机制可能参与前列腺素的抗溃疡和抗分泌作用。

Hara,N.,Hara,Y.,Goto,Y.: "Acid secretagogue mechanisms of a GABA-mimetic(PCPGABA)in the anesthetized rat:Effect of hypothermia" Life Sciences. (1990)

Hara,N.、Hara,Y.、Goto,Y.：“麻醉大鼠中 GABA 模拟物 (PCPGABA) 的酸分泌机制：低温的影响”生命科学。

Hara, N., Hara, Y., Natsume, Y. and Goto, Y.: "Gastric acid inhibitory action of a GABA-related compound, 3-amino-3-phenylpropionic acid, in the rat." European Journal of Pharmacology. (1990)

Hara, N.、Hara, Y.、Natsume, Y. 和 Goto, Y.：“GABA 相关化合物 3-氨基-3-苯基丙酸对大鼠的胃酸抑制作用。”

Goto,Y.,Ohno,H.,Tache,Y.: "Ulcer disease:New aspects of pathogenesis and pharmacology" CRC Press(Florida,U.S.A.), 460 (1989)

Goto,Y.、Ohno,H.、Tache,Y.：“溃疡病：发病机制和药理学的新方面”CRC Press（佛罗里达州，美国），460（1989）

Goto, Y. and Yamada, M.: "Quantitative assessment of mild stages of the experimental gastritis in the rat and effect of several types of antiulcer drugs." Scandinavian Journal of Gastroenterology. 24 suppl.162. 166-169 (1989)

Goto, Y. 和 Yamada, M.：“大鼠实验性胃炎轻度阶段的定量评估以及几种抗溃疡药物的效果。”