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Isolation and purification of gastric inhibitors from canine gastrointestinal mucosa

Isolation and purification of gastric inhibitors from canine gastrointestinal mucosa
犬胃肠粘膜胃抑制剂的分离纯化
批准号：
63044141
负责人：
GOTO Yoshiaki
金额：
$2.37万
依托单位：
Tokushima Bunri University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Overseas Scientific Survey.
财政年份：
1988
资助国家：
日本
项目状态：
已结题
起止时间：
1988 至 1989
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63044141/
关键词：
StomachGastric SecretionFundic Inhibitor

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英文摘要
Having shown that excision of the oxyntic mucosa of the dog stomach removes a powerful inhibitor, we attempted preliminary isolation of the inhibitor from canine fundic mucosa. The inhibitor was extracted from boiled, homogenized canine gastric mucosa in 2 % cold trifluoroacetic acid (TFA), centrifuged, filtrated and lyophilized. The lyophilized extract was dissolved in water and first purification on Sephadex G10. The inhibitor was found in the salt peak fractions on elution with 0.1 % TFA. These fractions were pooled, loaded onto a reverse-phase C18 column and purified by HPLC. Inhibitory activity of the fractions was tested on pentagastrin-stimulated acid secretion in the rat. The inhibitor is not retained on the HPLC and after ether extraction under both acidic and alkaline condition, the inhibitor was concentrated in aqueous phase of an aqueous/lipid solvent system. This suggests that the inhibitor is a charged, zwitterionic substance, possible a small protein
期刊论文(11)
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Goto, Y., Ohno, H. and Tache, Y.: Possible involvement of central mechanisms in antiulcer and antisecretory actions of prostaglandins. In ULCER DISEASE; New aspects of pathogenesis and pharmacology.CRC Press (Florida, U.S.A.), 229-234 (1989)
Goto, Y.、Ohno, H. 和 Tache, Y.：中枢机制可能参与前列腺素的抗溃疡和抗分泌作用。
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通讯作者：
Hara,N.,Hara,Y.,Goto,Y.: "Acid secretagogue mechanisms of a GABA-mimetic(PCPGABA)in the anesthetized rat:Effect of hypothermia" Life Sciences. (1990)
Hara,N.、Hara,Y.、Goto,Y.：“麻醉大鼠中 GABA 模拟物 (PCPGABA) 的酸分泌机制：低温的影响”生命科学。
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通讯作者：
Hara, N., Hara, Y., Natsume, Y. and Goto, Y.: "Gastric acid inhibitory action of a GABA-related compound, 3-amino-3-phenylpropionic acid, in the rat." European Journal of Pharmacology. (1990)
Hara, N.、Hara, Y.、Natsume, Y. 和 Goto, Y.：“GABA 相关化合物 3-氨基-3-苯基丙酸对大鼠的胃酸抑制作用。”
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通讯作者：
Goto,Y.,Ohno,H.,Tache,Y.: "Ulcer disease:New aspects of pathogenesis and pharmacology" CRC Press(Florida,U.S.A.), 460 (1989)
Goto,Y.、Ohno,H.、Tache,Y.：“溃疡病：发病机制和药理学的新方面”CRC Press（佛罗里达州，美国），460（1989）
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共 11 条
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    Investigation on ultimate behavior of continuous elevated-girder highway bridges by multi-directional shake table test and development of advanced seismic performance verification method
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      Grant-in-Aid for Scientific Research (A)
    • 资助金额：
      $30.87万
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      Nagoya Institute of Technology
    Study on the display methods of tactile guide maps for promoting one's spatial perception
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      23560754
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      Grant-in-Aid for Scientific Research (C)
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      $3.24万
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      2011
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    海外基金