BIOCHEMISTRY OF GLYCOLIPIDS IN GASTRIC SECRETION

胃分泌中糖脂的生物化学

• 批准号: 3227083
• 负责人: BRONISLAW LESZEK SLOMIANY
• 金额: $ 24.35万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3227083
• 关键词: acylation; antiulcer drug; chemical structure function; gas chromatography; gastric juice; gastric mucosa; gastrointestinal epithelium; glycolipids; glycoproteins; human subject; laboratory rat; pepsin; phosphatidylcholines; polymers; proteolysis; secretion; stomach disorder; sulfation; sulfotransferase

The long term objective of this project is to explore the biochemistry and function of glycolipids, phospholipids and neutral lipids in gastrointestinal mucus in health and disease, and to provide the information on the role of mucus lipids in gastric mucosal protection. The specific goals set for the proposed period are: to define the role of covalently bound fatty acids and associated lipids in the viscoelastic, permselective and hydrophobic behavior of mucus glycoproteins along the gastrointestinal tract, and in the susceptibility of these glycoproteins to various proteases; to ascertain the extent of changes occurring in the covalently bound and associated lipids, and in the physicochemical characteristics of gastric mucus glycoprotein in stomach pathology; to assess the effect of antiulcer drugs that enhance the mucosal defense and agents affecting the integrity of mucus gel on the proteolytic susceptibility and the hydrophobic, viscoelastic, and permselective properties of gastric mucus and mucus glycoprotein; to study the effect of antiulcer drugs that enhance the mucosal defense on the acylation of mucus glycoprotein with fatty acids by gastric mucosal cells in culture; to conduct the measurements of the mucus glycoprotein fatty acyltransferase enzyme activity in fundic and antral mucosal biopsies of patients with gastric disease in order to provide an insight into the changes taking place in gastric mucus gel in stomach pathology; and to investigate the extent of changes occurring in mucus and mucosa components of gastric mucosal barrier in disease by studying in human gastric mucosal biopsies the activity of sulfotransferase enzymes responsible for sulfation of structural and secretory glycolipids.

本项目的长期目标是探索 糖脂、磷脂和中性脂的生物化学和功能 健康和疾病中胃肠道粘液中的脂质， 提供有关粘液脂质在胃粘膜中作用的信息， 粘膜保护 为拟议的《公约》确定的具体目标 期间是：定义共价结合的脂肪酸的作用， 粘弹性、选择性渗透和 粘液糖蛋白沿着 胃肠道，并在这些敏感性 糖蛋白的各种蛋白酶;以确定程度 发生在共价结合和缔合的脂质中的变化， 以及胃粘液的理化特性 糖蛋白在胃病理学中的作用;评估 增强粘膜防御的抗溃疡药物和药剂 影响粘液凝胶的完整性， 敏感性和疏水性，粘弹性，和 胃粘液和粘液的选择性渗透特性 糖蛋白;研究抗溃疡药物的作用， 粘液糖蛋白酰化的粘膜防御 脂肪酸的胃粘膜细胞培养;进行 粘液糖蛋白脂肪酰基转移酶的测量 患者胃底和胃窦粘膜活检中的酶活性 与胃病的关系，以便深入了解 在胃病理学中发生在胃粘液凝胶中;以及 研究粘液和粘膜发生变化的程度 胃粘膜屏障成分在疾病中的作用 人胃粘膜活检组织中磺基转移酶的活性 负责结构和分泌硫酸化的酶 糖脂