BIOCHEMISTRY OF GLYCOLIPIDS IN GASTRIC SECRETION

这项计划的长远目标是探讨 糖脂、磷脂和中性脂肪的生物化学和功能 健康和疾病中胃肠粘液中的脂质，以及 提供有关粘液脂质在胃中的作用的信息 粘膜保护。为建议的 时间段是：定义共价结合脂肪酸的作用和 粘弹性、渗透性和选择性的相关脂类 粘液糖蛋白的疏水行为 胃肠道，以及这些疾病的易感性 糖蛋白对各种酶的作用；以确定 在共价结合和相关的脂类中发生的变化， 以及胃粘液的理化特性 糖蛋白在胃病理中的作用 增强粘膜防御的抗溃疡药物和制剂 粘液凝胶完整性对蛋白水解率的影响 磁化率和疏水性、粘弹性和 胃粘液和胃粘液的渗透选择特性 糖蛋白；研究抗溃疡药物增强 粘液糖蛋白酰化的粘膜防御作用 培养的胃粘膜细胞中的脂肪酸；进行 粘液糖蛋白脂肪酰基转移酶的测定 胃底和胃窦粘膜活检组织中的酶活性 胃病，以便提供对变化的洞察力 在胃病理中发生在胃粘液凝胶中； 观察粘液和粘膜的变化程度。 胃黏膜屏障成分在疾病中的研究 人胃粘膜活检组织中磺基转移酶的活性 负责结构和分泌物硫酸盐化的酶 糖脂。

The long term objective of this project is to explore the biochemistry and function of glycolipids, phospholipids and neutral lipids in gastrointestinal mucus in health and disease, and to provide the information on the role of mucus lipids in gastric mucosal protection. The specific goals set for the proposed period are: to define the role of covalently bound fatty acids and associated lipids in the viscoelastic, permselective and hydrophobic behavior of mucus glycoproteins along the gastrointestinal tract, and in the susceptibility of these glycoproteins to various proteases; to ascertain the extent of changes occurring in the covalently bound and associated lipids, and in the physicochemical characteristics of gastric mucus glycoprotein in stomach pathology; to assess the effect of antiulcer drugs that enhance the mucosal defense and agents affecting the integrity of mucus gel on the proteolytic susceptibility and the hydrophobic, viscoelastic, and permselective properties of gastric mucus and mucus glycoprotein; to study the effect of antiulcer drugs that enhance the mucosal defense on the acylation of mucus glycoprotein with fatty acids by gastric mucosal cells in culture; to conduct the measurements of the mucus glycoprotein fatty acyltransferase enzyme activity in fundic and antral mucosal biopsies of patients with gastric disease in order to provide an insight into the changes taking place in gastric mucus gel in stomach pathology; and to investigate the extent of changes occurring in mucus and mucosa components of gastric mucosal barrier in disease by studying in human gastric mucosal biopsies the activity of sulfotransferase enzymes responsible for sulfation of structural and secretory glycolipids.