BIOCHEMISTRY OF GLYCOLIPIDS IN GASTRIC SECRETION

胃分泌中糖脂的生物化学

• 批准号: 3227081
• 负责人: BRONISLAW LESZEK SLOMIANY
• 金额: $ 23.29万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3227081
• 关键词: acylation; antiulcer drug; chemical structure function; gas chromatography; gastric juice; gastric mucosa; gastrointestinal epithelium; glycolipids; glycoproteins; human subject; laboratory rat; pepsin; phosphatidylcholines; polymers; proteolysis; secretion; stomach disorder; sulfation; sulfotransferase

The long term objective of this project is to explore the biochemistry and function of glycolipids, phospholipids and neutral lipids in gastrointestinal mucus in health and disease, and to provide the information on the role of mucus lipids in gastric mucosal protection. The specific goals set for the proposed period are: to define the role of covalently bound fatty acids and associated lipids in the viscoelastic, permselective and hydrophobic behavior of mucus glycoproteins along the gastrointestinal tract, and in the susceptibility of these glycoproteins to various proteases; to ascertain the extent of changes occurring in the covalently bound and associated lipids, and in the physicochemical characteristics of gastric mucus glycoprotein in stomach pathology; to assess the effect of antiulcer drugs that enhance the mucosal defense and agents affecting the integrity of mucus gel on the proteolytic susceptibility and the hydrophobic, viscoelastic, and permselective properties of gastric mucus and mucus glycoprotein; to study the effect of antiulcer drugs that enhance the mucosal defense on the acylation of mucus glycoprotein with fatty acids by gastric mucosal cells in culture; to conduct the measurements of the mucus glycoprotein fatty acyltransferase enzyme activity in fundic and antral mucosal biopsies of patients with gastric disease in order to provide an insight into the changes taking place in gastric mucus gel in stomach pathology; and to investigate the extent of changes occurring in mucus and mucosa components of gastric mucosal barrier in disease by studying in human gastric mucosal biopsies the activity of sulfotransferase enzymes responsible for sulfation of structural and secretory glycolipids.

该项目的长期目标是探索 糖脂、磷脂和中性脂的生物化学和功能 健康和疾病中胃肠粘液中的脂质，以及 提供有关粘液脂质在胃中的作用的信息 粘膜保护。 为建议设定的具体目标 周期是：定义共价结合脂肪酸的作用和 粘弹性、选择性渗透和 粘液糖蛋白的疏水行为 胃肠道，以及这些的易感性 糖蛋白到各种蛋白酶；以确定程度 共价结合和相关脂质发生的变化， 以及胃粘液的理化特性 胃病理学中的糖蛋白；评估效果 增强粘膜防御能力的抗溃疡药及药剂 影响粘液凝胶的蛋白水解完整性 敏感性和疏水性、粘弹性和 胃粘液和粘液的选择性渗透特性 糖蛋白；研究增强抗溃疡药物的作用 粘膜对粘液糖蛋白酰化的防御作用 培养物中胃粘膜细胞的脂肪酸；进行 粘液糖蛋白脂肪酰基转移酶的测量 患者胃底和胃窦粘膜活检中的酶活性 患有胃病，以便深入了解胃病的变化 胃病理学中发生在胃粘液凝胶中；并到 研究粘液和粘膜发生变化的程度 通过研究胃粘膜屏障在疾病中的组成部分 人胃粘膜活组织检查磺基转移酶的活性 负责结构和分泌硫酸化的酶 糖脂。