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A Morphological and Functional Study of Choroidal Melanocytes

脉络膜黑素细胞的形态和功能研究

基本信息

批准号：
63440066
负责人：
TOKORO Takashi
金额：
$ 10.18万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1991
项目状态：
已结题

项目摘要

This study was performed to clarify the morphology and function of the choroidal melanocytes. Eyes of macaque monkeys, pigmented rabbits and cats were used for the morphological study. In the suprachoroidea, the fibroblasts had long thin cytoplasmic processes and were in contact with neighboring cells by intercellular junctions, thus forming a network.Melanocytes were wedged between the thin processes of fibroblasts, and surrounded by collagen fibers and elastic fibers. In some areas, there were interfibroblastic spaces, which were devoid of fiber elements and filled with amorphous substances. When the tracers such as a suspension of activated carbon were injected into the anterior chamber, these tracers were detected in the interfibroblastic spaces at the suprachoroid. So, these spaces may function as the lymphatic route. Furthermore, we examined VIP-positive nerve fiber in the choroid of human enucleated eye by electron microscopical immunohistochemstry. Although VIP-positive nerve fibers were observed in the suprachoroid and inner scleral layers near choroidal melanocytes, no direct contact between VIP-positive nerve fibers and melanocytes could be found.In addition, electron microscopy revealed the gap junction between choroidal melanocytes and physiological methods demonstrated dye coupling and electrical coupling between choroidal melanocytes in rabbit's eyes. Thus it can be hypothesized that a group of choroidal melanocytes may function as a unit by exchanging ions and bioactive substances through their intercellular communication system, the gap junction. Furthermore, we investigated the suprachoroid uptake of extracellular calcium ions by using fura-2 and found that the intercellular calcium ions concentration was significantly increased by ATP superfusion. So, one of the functions of melanocytes and fibroblasts may be to regulate the extracellular fluid in the choroid.

本研究旨在阐明脉络膜黑素细胞的形态和功能。猕猴、有色兔和猫的眼睛用于形态学研究。脉络膜上膜成纤维细胞具有细长的胞质突起，并通过细胞间连接与相邻细胞接触，形成网状结构，黑素细胞夹在细胞突起之间，周围为胶原纤维和弹性纤维所包围。在某些区域，有成纤维细胞间的空间，这是缺乏纤维元素，充满了无定形物质。当示踪剂如活性炭悬浮液被注入前房时，这些示踪剂在脉络膜上的成纤维细胞间隙中被检测到。所以，这些空间可能起着淋巴通路的作用。此外，我们还用电镜化学方法观察了人眼球摘除脉络膜中VIP阳性神经纤维。在兔眼脉络膜上膜和内巩膜层，虽然在脉络膜黑素细胞附近观察到VIP阳性神经纤维，但未发现VIP阳性神经纤维与黑素细胞之间的直接接触，电镜观察到脉络膜黑素细胞之间存在差距连接，生理学方法证实了脉络膜黑素细胞之间存在染料偶联和电偶联。因此，可以推测，一组脉络膜黑素细胞可能作为一个单位，通过交换离子和生物活性物质，通过它们的细胞间通讯系统，差距连接。此外，我们研究了脉络膜上的细胞外钙离子的摄取，通过使用Fura-2，发现细胞间钙离子浓度显着增加ATP灌流。因此，黑素细胞和成纤维细胞的功能之一可能是调节脉络膜中的细胞外液。