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Study about Regulation of Scleral Growth-using Experimental Myopia-

巩膜生长调节研究-利用实验性近视-

基本信息

批准号：
04454438
负责人：
TOKORO Takashi
金额：
$ 4.48万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

(1) Relationship between method of visual deprivation and induction of myopia : White Leghorn male chicks wearing plastic translucent or black goggles monocularyly showed high myopia with excessive axial elongation under 12hr-light/12hrs-dark cycles. When wearing plastic translucent or black goggles binocularly also showed high myopia with excessive axial elongation under 12hrs-light/12hrs-dark cycles. When wearing translucent or black plastic goggles monocularly did not show any axial elongation under 24hrs-dark condition.(2) Histological study : In the posterior segment of experimentally myopic sclera, expression of PCNA,TGFbeta、IGF-II,decorin, phosphotyrosine increased by immunohistochemical technique. These results suggest that cell prolioferation and matrix synthesis are increased in the posterior segment of experimentally myopic sclera, which could be regulated by such growth factors as TGFbeta、IGF-II.Electron microscopic observation suggested that active remodelling occurs in th … More e myopic cartilaginous sclera and suggested the thinner changes of collagen fibrils in the myopic fibrous sclera.(3) Biochemical analysis : TGFbeta and bFGF were extracted and were quantitatively analyzed by ELISA.In the posterior segment of experimentally myopic eyes, TGFbeta increased but bFGF was decreased.(4) Investigation by tissue culture : Cell proliferation of scleral chondrocytes and scleral fibroblasts derived chick embryos was stimulated by such growth factors as TGFbeta , bFGF,TGFalpha、IGF-II,IGF-I and PDGF.Cell proliferation of these scleral cells was also stimulated by co-cultured RPE cells. And this co-culture system revealed that the target of inhibitory effects of apomorphine on scleral growth could be RPE.(5) Tension experiment with bovine choroid : The degree of stretch was greater in the longitudinal direction than in the circular direction. Thus, it may be possible that the tension becomes one of the factors of axial elongation in the direction of the posterior pole.(6) Experimental myopia in monkeys : One of the monkeys with high myopia by visual deprivation by lid-suture, showed chorioretinal atrophy in his myopic eyes. Histological observation suggested that aging playd a role in that pathological change. Less

(1)视觉剥夺方法与近视诱导的关系：白色来航公雏鸡单眼戴塑料半透明或黑色护目镜，在12小时光照/12小时黑暗循环下，表现为高度近视，眼轴过度伸长。当戴塑料半透明或黑色护目镜时，在12小时光照/12小时黑暗循环下，双眼也显示出高度近视和过度的轴向伸长。当戴半透明或黑色塑料护目镜时，在24小时黑暗条件下，单眼没有显示出任何轴向伸长。(2)组织学研究：免疫组化结果显示，实验性近视眼巩膜后段PCNA、TGF β、IGF-II、核心蛋白聚糖、磷酸酪氨酸表达增加。结果提示，实验性近视眼巩膜后段细胞增殖和基质合成增加，可能受TGF β、IGF-Ⅱ等生长因子的调节。 ...更多信息近视性软骨性巩膜胶原纤维变薄。(3)生化分析：提取TGF β和bFGF，用ELISA法定量分析，结果显示：在实验性近视眼眼后段，TGF β升高，bFGF降低。(4)组织培养研究：TGF β、bFGF、TGF α、IGF-II、IGF-I和PDGF等生长因子刺激鸡胚巩膜软骨细胞和巩膜成纤维细胞增殖，与RPE细胞共培养也可刺激巩膜软骨细胞和巩膜成纤维细胞增殖。该共培养体系揭示了阿扑吗啡抑制巩膜生长的作用靶点可能是RPE。(5)牛脉络膜拉伸实验：纵向拉伸程度大于环向。因此，张力可能成为后极方向上轴向伸长的因素之一。(6)猴实验性近视：其中一只用眼睑缝合法造成高度近视的猴，其近视眼出现脉络膜视网膜萎缩。组织学观察表明，衰老在该病理变化中起一定作用。少