Expression and roles of growth factors in experimental myopic

生长因子在实验性近视眼中的表达及作用

• 批准号: 07407049
• 负责人: TOKORO Takashi
• 金额: $ 3.71万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07407049/
• 关键词: Experimental Myopia; Sclera; vetina; dopamine; growth factor; differentical display; mechanical strain; differential display; 青色光; 視性刺激遮断; 細膜; 網膜色素上皮; TGFβ; レチノイン酸

(1) Unknown substances regulating ocular growthDifferential display cloning was conducted between the normal retina and the form-deprived retina. 46 bands were lsolated which were expressed more strongly in the normal retina than in the deprived retina. Following reamplification, dot blot differential hybridization and northern blot selected 3 cDNA clones. Sequence homology analysis revealed that those 3 cDNA clones have unknown sequences. Now, we are investigating about those 3 cDNA clones.(2) Involvement of growth factors and retinoic acidImmunohistochemical analyzes of experimental myopic chickens revealed that TGF-beta and IGF II are increased in the myopic sclera. Biochemical analyzes showed that TGF-beta is increased and bFGF is decreased in the myopic sclera and that TGF-beta is also increased in the myopic retina-chorold. RT-PCR-ELISA revealed that expression of retinoic acid receptor-beta is increased in the myopic sclera and HPLC analysis showed that retinoic acid is increase … More d in the myopic retina.Tissue culture experiments showed that the retinal pigment epithelium (RPE) releases some growth regulating substances.(3) Regulation of growth regulating mechanism of theRPE-relationship to the blue light exposure-Previous reports showed that visible light of short wave length "blue light" is toxic to the retina. But the mechanism of the toxicity has been unclear. We established an in virto model of exposure of blue light to the RPE and investigated the morphology of degeneration of the RPE by blue light in detail. We are investigating fuctional changes of the RPE by blue light exposure.(4) Involvement of retinal dopamine in the roles of growth factorsRetinal dopamine has been reported to be decreased in the myopic eye. We demonstrated that apomorphine, dopamine agonist, inhibits growth stimulating effects of RPE on scleral cells in vitro. Now, we are investigating about roles of Muller cells. At first, we revealed that dopamine inhibits cell growth of Muller cells.(5) Involvement of mechanical strain in the roles of growth factorsOcular hypertension and accommodation have been reported to play a role in the ocular enlargement. We investigated the effects of mechanical strain on the biochemical changes of the scleral cells and the RPE cells in vitro. Mechanical strain affected cell growth of scleral cells and the release of growth factors from the RPE cells. Less

(1)调节眼睛生长的未知物质在正常视网膜和形状剥夺视网膜之间进行差异显示克隆。分离出 46 个条带，这些条带在正常视网膜中的表达比在缺失的视网膜中更强。再扩增后，通过斑点印迹差异杂交和Northern印迹选择了3个cDNA克隆。序列同源性分析显示，这3个cDNA克隆具有未知序列。目前，我们正在对这3个cDNA克隆进行研究。(2)生长因子和视黄酸的参与对实验性近视鸡进行免疫组化分析，发现近视巩膜中TGF-β和IGF II增加。生化分析表明，近视巩膜中 TGF-β 增加，bFGF 减少，近视视网膜脉络膜中 TGF-β 也增加。 RT-PCR-ELISA显示近视巩膜中视黄酸受体-β表达增加，HPLC分析显示近视视网膜中视黄酸增加。组织培养实验表明视网膜色素上皮(RPE)释放一些生长调节物质。(3)RPE生长调节机制的调节-与蓝光的关系 曝光——此前的报道表明，短波长“蓝光”的可见光对视网膜有毒。但其毒性机制尚不清楚。我们建立了蓝光照射 RPE 的体外模型，并详细研究了蓝光对 RPE 变性的形态。我们正在研究蓝光照射对RPE的功能变化。(4)视网膜多巴胺参与生长因子的作用 据报道，近视眼中视网膜多巴胺减少。我们证明阿扑吗啡（多巴胺激动剂）在体外抑制 RPE 对巩膜细胞的生长刺激作用。现在，我们正在研究穆勒细胞的作用。首先，我们发现多巴胺抑制Muller细胞的生长。(5)机械应变参与生长因子的作用据报道，高眼压和调节在眼球增大中发挥作用。我们在体外研究了机械应变对巩膜细胞和 RPE 细胞生化变化的影响。机械应变影响巩膜细胞的生长以及 RPE 细胞生长因子的释放。较少的

项目成果

Seko Y.et al.: "Retinoic acidincreases in theretina of the chick with form-dipvivation myoria" Ophthalmic Res. (in press). (1998)

Seko Y. 等人：“患有形态退化性肌病的小鸡视网膜中的视黄酸增加”眼科研究。

Seko Y. et al: "Retinoic acid increnses in the retina of the thick with form diprivition onyopia" Ophthalmic Res.(in press). (1998)

Seko Y. 等人：“视黄酸在厚视网膜中增加，形成剥夺性强视”眼科研究（正在出版）。

世古裕子: "眼科診療Q&A 近視発生の病態" 六法出版者(in press), (1998)

Yuko Seko：“眼科问答：近视的病理状况”Rokupo Publishing（正在印刷），（1998）

草刈匡世: "眼科臨床プラクティス22.「やさしい眼の生化学・分子生物学」強膜の組成と成長因子" 文光堂, 4 (1996)

Masayo Kusakari：“眼科临床实践 22.‘简易眼生物化学和分子生物学’巩膜成分和生长因子”Bunkodo，4 (1996)

Yuko Seko: "Influence of bFGF as a potent growth stimulator and TGF-β as a growth regulator on scleral chondrocytes and scleral fibroblasts in vitro" Ophthalmic Res.27. 144-152 (1995)

Yuko Seko：“bFGF 作为有效生长刺激剂和 TGF-β 作为生长调节剂对体外巩膜软骨细胞和巩膜成纤维细胞的影响”眼科研究 144-152 (1995)。