Expression and roles of growth factors in experimental myopic

(1)调节眼部生长的未知物质对正常视网膜和形态缺失视网膜进行了差异显示克隆。分离到46个条带，正常视网膜中表达较缺失视网膜强烈。再扩增后，选择3个cDNA克隆进行斑点杂交和northern杂交。序列同源性分析表明，这3个cDNA克隆均有未知序列。现在，我们正在研究这3个cDNA克隆。(2)对实验性近视鸡的生长因子和视黄酸的免疫组化分析显示，近视巩膜中tgf - β和IGF II升高。生化分析显示，近视巩膜中tgf - β升高，bFGF降低，近视视网膜中tgf - β升高。RT-PCR-ELISA检测显示近视巩膜中维甲酸受体- β的表达增加，HPLC分析显示近视视网膜中维甲酸的表达增加。组织培养实验表明，视网膜色素上皮（RPE）释放出一些生长调节物质。(3) therpe的生长调节机制与蓝光暴露的关系——以往的报道表明，短波长的可见光“蓝光”对视网膜有毒性。但其毒性机制尚不清楚。我们建立了蓝光照射下RPE的虚拟模型，详细研究了蓝光照射下RPE变性的形态学。我们正在研究蓝光照射下RPE的功能变化。(4)视网膜多巴胺参与生长因子的作用据报道，近视眼的视网膜多巴胺减少。在体外实验中，我们证明了多巴胺激动剂阿波啡可以抑制RPE对巩膜细胞的生长刺激作用。现在，我们正在研究穆勒细胞的作用。首先，我们发现多巴胺抑制Muller细胞的生长。(5)机械应变参与生长因子的作用。据报道，眼压增高和调节在眼增大中起作用。我们在体外研究了机械应变对巩膜细胞和RPE细胞生化变化的影响。机械应变影响巩膜细胞的生长和RPE细胞中生长因子的释放。少

(1) Unknown substances regulating ocular growthDifferential display cloning was conducted between the normal retina and the form-deprived retina. 46 bands were lsolated which were expressed more strongly in the normal retina than in the deprived retina. Following reamplification, dot blot differential hybridization and northern blot selected 3 cDNA clones. Sequence homology analysis revealed that those 3 cDNA clones have unknown sequences. Now, we are investigating about those 3 cDNA clones.(2) Involvement of growth factors and retinoic acidImmunohistochemical analyzes of experimental myopic chickens revealed that TGF-beta and IGF II are increased in the myopic sclera. Biochemical analyzes showed that TGF-beta is increased and bFGF is decreased in the myopic sclera and that TGF-beta is also increased in the myopic retina-chorold. RT-PCR-ELISA revealed that expression of retinoic acid receptor-beta is increased in the myopic sclera and HPLC analysis showed that retinoic acid is increase … More d in the myopic retina.Tissue culture experiments showed that the retinal pigment epithelium (RPE) releases some growth regulating substances.(3) Regulation of growth regulating mechanism of theRPE-relationship to the blue light exposure-Previous reports showed that visible light of short wave length "blue light" is toxic to the retina. But the mechanism of the toxicity has been unclear. We established an in virto model of exposure of blue light to the RPE and investigated the morphology of degeneration of the RPE by blue light in detail. We are investigating fuctional changes of the RPE by blue light exposure.(4) Involvement of retinal dopamine in the roles of growth factorsRetinal dopamine has been reported to be decreased in the myopic eye. We demonstrated that apomorphine, dopamine agonist, inhibits growth stimulating effects of RPE on scleral cells in vitro. Now, we are investigating about roles of Muller cells. At first, we revealed that dopamine inhibits cell growth of Muller cells.(5) Involvement of mechanical strain in the roles of growth factorsOcular hypertension and accommodation have been reported to play a role in the ocular enlargement. We investigated the effects of mechanical strain on the biochemical changes of the scleral cells and the RPE cells in vitro. Mechanical strain affected cell growth of scleral cells and the release of growth factors from the RPE cells. Less