Application of New Genetic Markers on Proteins, Enzymes and DNA to Forensic Medicine.

蛋白质、酶和 DNA 上的新遗传标记在法医学中的应用。

• 批准号: 01304036
• 负责人: HARADA Shoji
• 金额: $ 7.42万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01304036/
• 关键词: Genetic polymorphism; Probe; Blood stain; Paternity testing; PCR; DNA fingerprinting; Serum protein; Individual identification; DNA多型; pHYN22; MCT118; VNTR; GStπ gene; ORM型; Factor1; 唾液アミラ-ゼ; フィンガ-プリント法; PCR法; 蛋白多型; 人獣鑑定; 性別鑑定; RFLP_S

l. Application of Genetic Markers on DNAGenetic palymorphism detected by using different probes were applied to paternity testing of the family in dispute to obtain the data base in Japan. The probes pYNZ22, pYN22, MR24/1, 3'-Globin, Mucin and Ha-ras were used to detect the single locus DNA polymorphism. It was found by the statistical analysis that these probes supply very useful information for paternity testing. Moreover, VNTR of 5'-flanking region of GST pi gene showed a high heterozigosity (83.3%) in Japanese using PCR technology.An efficient method for isolating DNA from the forensic samples such as blood stains, bones and tissues were established to make individual identification. The isolated DNA samples were subjected to PCR for identifying VNTR on MCT 118 and ApoB and it was confirmed that these markers are very effective for forensic analysis. Also, pYNH24 probe was informative in the bone and tissue analysis. DNA fingerprinting detected by the single and multi loci probes was applied to testing in paternity cases in Japan.2. Genetic Markers of Proteins and Enzymes.We focused the serum proteins which have not yet studied in Japanese, although high polymorphisms have been reported in Caucasoid. The data concerning ZAG, IF, ORM, AHSG, Factor B have been obtainedin our country as well as in other Asian population, in addition to the racial differences of gene frequencies of these serum proteins. Moreover, Factor I, DR and Factor H were found to be useful markers for paternity testing.Genetic analysis of saliva amylase variants has been done including the study of the difference among human and non-human primates. LDH isozyme pattern of the injured body was investigated from aspect of forensic analysis.

L。遗传标记在DNA检测中的应用不同探针检测的遗传多态现象在日本应用于有争议的家庭的亲子鉴定，以获得数据库。用pYNZ22、pYN22、MR24/1、3‘-Globin、Mucin和Ha-ras探针检测单基因座DNA多态性。通过统计分析发现，这些探针为亲子鉴定提供了非常有用的信息。此外，利用聚合酶链式反应技术对日本人GST pi基因5‘-侧翼区进行了高异质性分析(83.3%)，建立了一种从血痕、骨骼和组织等法医标本中提取DNA的有效方法，用于个体鉴定。对分离的DNA样品进行了MCT 118和ApoB上的VNTR的PCR检测，证实了这些标记在法医分析中是非常有效的。此外，pYNH24探针在骨和组织分析中也提供了丰富的信息。将单、多位点探针检测的DNA指纹图谱应用于日本的亲子鉴定。蛋白质和酶的遗传标记。我们关注尚未在日本研究的血清蛋白质，尽管在高加索人中已经报道了高度多态。ZAG、IF、ORM、AHSG、B因子等血清蛋白基因频率的种族差异已在我国和其他亚洲人群中获得。此外，因子I、DR和因子H被发现是亲子鉴定的有用标记。唾液淀粉酶变异的遗传分析已经完成，包括研究人类和非人类灵长类之间的差异。从法医分析的角度对损伤身体的乳酸脱氢酶同工酶进行分析。

项目成果

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Masayuki Natani、Kaoru Osaka、Tsuyoshi Yokoi、Koichi Hiraiwa、Yasuhiro Aoki、Takehiko Takatori：“使用单位点 DNA 探针进行亲子鉴定”《日本法医学杂志》45. 138-145 (1991)。

吉岡 尚文,目黒 ひとみ,横井 毅,森田 香,那谷 雅之,匂坂 馨: "血清ハプトグロビン欠損の遺伝学的検討" 生物物理化学. 35. 63-67 (1991)

Naofumi Yoshioka、Hitomi Meguro、Takeshi Yokoi、Kaoru Morita、Masayuki Natani、Kaoru Osaka：“血清触珠蛋白缺乏症的遗传学研究”生物物理化学 35. 63-67 (1991)。