IN VITRO CULTURE OF SHOOT-TIPS AND THEIR COLD AND FREEZING TORELANCE AND THE POSSIBILITIES OF CRYOPRESERVATION IN LIQUID NITROGEN

芽尖的体外培养及其耐冷和冷冻能力以及液氮冷冻保存的可能性

• 批准号: 63560018
• 负责人: KATANO Manabu
• 金额: $ 1.28万
• 依托单位: KYUSHU TOKAI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560018/
• 关键词: SHOOT-TIP CULTURE; CRYOPRESERVATION; HERBACEOUS PLANTS; WOODY PLANTS; MEDIUM; INORGANIC SALTS; SEASONAL CHANGES; MEDIUM BACKGROUND; 耐凍性

Shoot-tip culture both 43 herbaceous plant species belonging to 17 genera, 10 families and 14 woody species to 13 genera,12 families were conducted from 1988 to 1989 on the following four kinds of media:combination of two kinds of inorganic salts, i.e. thick Murashige & Skoog's(1962) and thin Gamborg'S(1966) and two kinds of plant hormones, i.e. BAP(1mg/l) and IBA(0.2mg/l). Successful results of primary and sub-culture were gained on media with BAP more than with IBA,regardless of herbaceous and woody plants. In general, MS salts brought better growth in herbaccous plants, on the other hand, Gamborg's succeeded in woody plants. Medium background in primary culture heavily influenced succeeding shoot growth in subculture. Selection of inorganic salts was more important factor than that of plant hormones. In case of Japanese flowering cherries, seasonal changes of rate of shoot-tip survival in primary culture were clearly observed.Dormant shoot-tips of barley, white clover and Japanese flowering cherries could be survived after immersion in liquid nitrogen.

1988 ~ 1989年，对10科17属43种草本植物和12科13属14种木本植物的茎尖进行了培养，采用两种无机盐（Murashige & Skoog's（1962）和Gamborg's（1966））和两种植物激素（BAP（1 mg/l）和IBA（0.2mg/l）的组合培养基。无论是草本植物还是木本植物，在原代培养和继代培养中，BAP的成功率均高于IBA。一般来说，MS盐在草本植物中带来更好的生长，另一方面，Gamborg在木本植物中取得了成功。原代培养的培养基背景对继代苗的生长影响很大。无机盐的选择比植物激素更重要。以樱花为例，茎尖初代培养成活率有明显的季节变化，大麦、白色三叶草和樱花的休眠茎尖在液氮中浸泡后均能成活。

项目成果

片野学・田中裕二・栗原浩: "ホワイトクロ-バ-の茎頂培養ならびに茎頂の液体窒素中における凍結保存" 日本作物学会九州支部会報. 57. (1990)

Manabu Katano、Yuji Tanaka、Hiroshi Kurihara：“白三叶草茎尖的培养和液氮中茎尖的冷冻保存”日本作物学会九州分会通报 57。（1990）。

Katano,M.and R.IRIE: "Micropropagation of Japanese flowering cherry“Someiーyoshino"and survival of shootーtips after immersion in liquid nitrogen" Proc.Fac.Agric.Kyushu Tokai University. 10. (1991)

Katano, M. 和 R.IRIE：“日本樱花“染井吉野”的微繁殖和浸入液氮后茎尖的存活”Proc.Fac.Agric.九州东海大学 10。（1991 年）

KATANO, M. and R.IRIE: "Micropropagation of Japanese flowering cherry "Somei-yoshino" and survival of shoot-tips after immersion in liquid nitrogen" Proc.Fac.Agric.Kyushu Tokai Univ.10, 1991.

KATANO, M. 和 R.IRIE：“日本樱花“染井吉野”的微繁殖和浸入液氮后茎尖的存活”Proc.Fac.Agric.Kyushu Tokai Univ.10，1991。

KATANO, M., Y.KENDA and H.KURIHARA: "Shoot-tip culture of several species of herbaceous and woody plants on different formula of medium" Rep.Kyushu Br.Crop Sci.Soc. Japan 55:56-61, 1988.

KATANO, M.、Y.KENDA 和 H.KURIHARA：“在不同配方的培养基上进行几种草本和木本植物的茎尖培养”Rep.Kyushu Br.Crop Sci.Soc。

片野学・見田良彦・栗原浩: 日本作物学会九州支部会報. 55. 56-61 (1988)

Manabu Katano、Yoshihiko Mita、Hiroshi Kurihara：日本作物科学学会九州分会通报 55. 56-61 (1988)。