Localization and dynamics of oxytocin mRNA as revealed by in situ hybridization histochemistry.

氧化酶神经元系统基因表达的机理是通过在原位杂交历史学中使用的。第一次尝试是为了对氧霉素mRNA进行特定的探测，因为核肽序列的类似序列对vasopressin mRNA进行分析，这反映了对特定杂交信号的评估。在设计氧化物mRNA的特定核苷酸序列后， 25- 30聚体寡核苷酸序列是合成的。一种特殊的注意力是为了使探针至少具有同源性，从而产生血管紧张素和其他生物活性物质的mRNA。The probes were labeled by radioactive mononucleotides such as ^3 H·dCTP， ^<35>S·dATP。第二次尝试是为了调查雌激素和/或雌激素对氧化物基因表达的影响。Estrogen treatment did not induce changes of oxytociin mRNA both in neurons of the paraventricular and supraoptic nuci。However、雌激素和progesterone治疗导致在神经元中的氧ytocin mRNA的显著增加，部分在准核的肠道亚核中。神经元in the supraoptic Nucleus did not show任何oxytocin mRNA的变化，estrogen和progesterone治疗。第三次实验通过对氧蛋白的抑制作用进行了研究，当氧蛋白神经元细胞体内的增强电子活动时，进行了氧化蛋白mRNA含量的快速增加。为了刺激催产素释放，纳洛酮在肠道内吗啡灌注后给予女性老鼠。纳洛酮增加了40个等离子体中的催产素含量，至少在吗啡中毒大鼠中停留40分钟。纳洛昔酮对标记细胞中的氧素mRNA含量没有明显的影响，对标记细胞的比例没有影响，我们将基因表达的氧素神经元的机制结合在一起，在核、细胞体和终端水平上存在差异。

Mechanisms of the gene expression of oxytocin neuron system were investigated by using in situ hybridization histochemistry. The first attempt was done to make specific probes for oxytocin mRNA because of the similar sequence of nucleotides to vasopressin mRNA, and this reflected the evaluation of specific hybridization signals to analyze. After designing the specific nucleotide sequence for oxytocin mRNA, 25-30mer oligonucleotides were synthesized. A particular attention was paid to make probes having least homology to mRNAs of vasopressin and other bioactive substances. The probes were labeled by radioactive mononucleotides such as ^3H・dCTP, ^<35>S・dATP. Second attempt was done to investigate the effects of estrogen and/or progesterone to oxytocin gene expression. Estrogen treatment did not induce changes of oxytociin mRNA both in neurons of the paraventricular and supraoptic nuclei. However, estrogen and progesterone treatment caused a significant increase of oxytocin mRNA in neurons, particularly in the ventromedial subnucleus of the paraventricular nucleus. Neurons in the supraoptic nucleus did not show any changes of oxytocin mRNA by estrogen and progesterone treatment. Third experiment was carried out to investigate whether a substained increase in oxytocin secretion, with or without enhanced electrical activity of the cell bodies of oxytocin neurons, leads to a rapid increase in oxytocin mRNA content in these neurons. To stimulate oxytocin release, naloxone was given to female rats after intracerebroventricular morphine infusion. Naloxone increased oxytocin content in plasma 40 fold for at least 40 min in morphine-infused rats. Naloxone had no significant effect on the oxytocin mRNA content in labeled cells in the supraoptic nucleus, and no effect on the proportion of labeled cells.We concluded that there exist differences in mechanisms of gene expression of oxytocin neurons at the nuclear, cell body and terminal levels.