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MOLECULAR AND CELLULAR APPROACH FOR THE ANALYSIS ON THE REGULATION OF HYPOTHALAMIC CONTROL AND LATERALIZATION

下丘脑控制和侧化调节的分子和细胞方法分析

基本信息

批准号：
03454120
负责人：
KAWATA Mitsuhiro
金额：
$ 3.71万
依托单位：
KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

Endocrine organs are regulated by the control of the brain. This regulation is classified into two parts, humoral and neurogenic. The present study was undertaken to elucidate the molecular and cellular control of hypothalamic neurons the endocrine cells, particularly emphasized on the role of lateralization in the hypothalamus. To investigate the neuronal projection to and from the spinal cord, cholera toxin was applied to the peripheral tissues and retrograde transport to the neurons in the spinal cord and ganglia was visualized. Ventral and dorsal roots sections induced the changes of the distribution of chemical transmitter substances, such as CGRP, enkephalin, substance P, serotonin and galanin. CGRP-and substance P-immunoreactive nerve fibers were significantly decreaseat the operated side by the root section, whereas enkephalin-,serotonin-,and galanin-immunoreactive nerve fibers did not show any changes ispilaterally as well as contralaterally. Spinal cords from the fetus or neonatal period were transplanted into the anterior chamber of the eye, and the changes of the transmitter substances were observed in a isolated condition. In this condition, there were two types of the changes in the distributional pattern of immunoreactive nerve fibers; some fibers showed different distribution compared to the control, but some did not. Steroid hormone receptor-containing cells were distributed in the hypothalamus, particularly in the preoptic area. Long term treatment of estrogen induced significant increase of the number of CGRP-and enkephalin-immunoreactive nerve fibers in the preoptic area, and these cells contained estrogen-receptor in their nucleus. On the contrary, dopaninergic neurons showed an apparent decrease of the number of immunoreactive fibers in the preoptic area. We developed the culture system, and now trying to investigate the effect of steroid hormone on the hypothalamic neurons in vitro.

内分泌器官受大脑的控制。这种调节分为两部分：体液调节和神经源性调节。本研究旨在阐明下丘脑神经元和内分泌细胞的分子和细胞控制，特别强调下丘脑侧化的作用。为了研究进出脊髓的神经元投射，将霍乱毒素施加到周围组织，并观察到脊髓和神经节中神经元的逆行转运。腹根和背根切片引起CGRP、脑啡肽、P物质、血清素和甘丙肽等化学递质物质分布的变化。 CGRP-和P物质-免疫反应性神经纤维在手术侧根部部分显着减少，而脑啡肽-、血清素-和甘丙肽-免疫反应性神经纤维在同侧和对侧均未显示任何变化。将胎儿或新生儿时期的脊髓移植到眼前房，在隔离条件下观察递质物质的变化。在这种情况下，免疫反应神经纤维的分布模式有两种类型的变化：与对照相比，一些纤维显示出不同的分布，但有些则没有。含有类固醇激素受体的细胞分布在下丘脑，特别是视前区。长期雌激素治疗会导致视前区 CGRP 和脑啡肽免疫反应性神经纤维数量显着增加，这些细胞的细胞核中含有雌激素受体。相反，多巴能神经元显示视前区免疫反应纤维数量明显减少。我们开发了培养系统，现在试图在体外研究类固醇激素对下丘脑神经元的影响。