Effects of perinatal steroid hormonal changes on the cytoarchitecture of brain

围产期类固醇激素变化对大脑细胞结构的影响

• 批准号: 09480214
• 负责人: KAWATA Mitsuhiro
• 金额: $ 8.06万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480214/
• 关键词: sex hormone; estrogen receptor; postnatal development; apoptosis; over expression; preoptic area; sexual dimorphism; GFP; ステロイドホルモンレセプター; ポリクローナル抗体; プローブ; 免疫細胞化学法; in situハイブリダイゼーション法; 脳内分布; 部位特異的発現

To elucidate the mechanism of the formation of sexual dimorphism, we examined the number of apoptotic cells in the medial preoptic nucleus in male and female rats of various developing stages. Many apoptotic cells in the postnatal day 1 and 4 were found in the preoptic periventricular nucleus and its number was significantly larger in male than in female. The number of apoptotic cells in the medial preoptic nucleus was increased in the postnatal day 4 and 7 and significantly larger in female than in male. It was found that the distribution of apoptotic cells depends on the sexuality, the location of the area and postnatal period, indicating that the apoptosis contributes the formation of sexual dimorphism in the medial preoptic area. The medial preoptic area contains highest number of estrogen receptors. Therefore we expanded our experiments to investigate the subcellular localization of estrogen receptor in cultured cells, in which estrogen receptor linked to GFP was over expressed. With or without ligand, the subcellular localization of estrogen receptor was localized in the nucleus, not in the cytoplasm.It was concluded that the sexual dimorphism in the brain was due, at least in part, to the apotosis of the neurons in the specific areas and these apoptosis was strongly linked to the gene expression of steroid hormone receptor.

为了阐明两性异形形成的机制，我们检测了不同发育阶段雄性和雌性大鼠视前内侧核中凋亡细胞的数量。生后第1、4天，视前室周核内可见大量凋亡细胞，且雄性明显多于雌性。视前内侧核内凋亡细胞数在生后4、7日龄时明显增多，且雌鼠明显多于雄鼠。结果发现，凋亡细胞的分布与性别、区域位置和出生后时间有关，表明凋亡参与了视前内侧区性二型的形成。内侧视前区含有最多的雌激素受体。因此，我们扩大了我们的实验，以研究雌激素受体在培养细胞中的亚细胞定位，其中雌激素受体连接到GFP过表达。雌激素受体的亚细胞定位与雌激素受体的基因表达密切相关，雌激素受体的亚细胞定位与雌激素受体的基因表达密切相关，雌激素受体的亚细胞定位与雌激素受体的基因表达密切相关。

项目成果

Hu Z.,Yuri K.,Ozawa H.,Lu H.,Kawata M.: "The in vivo time course for elimination of adrenalectomy-induced apoptotic profiles from the granule cell layer of the rat" J.Neurosci.17. 3981-3989 (1997)

Hu Z.、Yuri K.、Ozawa H.、Lu H.、Kawata M.：“消除大鼠颗粒细胞层肾上腺切除诱导的细胞凋亡特征的体内时间过程”J.Neurosci.17。

Hu Z ., Ito T., Yuri K., Chang X., Ozawa H., Kawata M.: "In vivo time course of morphological changes and DNA degradation during the degeneration of castration-induce apoptotic cells" Cell Tissue Res.294. 153-160 (1998)

Hu Z.、Ito T.、Yuri K.、Chang X.、Ozawa H.、Kawata M.：“去势诱导凋亡细胞变性过程中形态变化和 DNA 降解的体内时间过程”Cell Tissue Res.294

森田規之,伊藤隆男,河田光博: "脱アイソトープ実践プレトコール,カラーin situハイブリダイゼーション" 秀潤社,東京, 269-299 (1998)

Noriyuki Morita、Takao Ito、Mitsuhiro Kawata：“同位素去除实践、彩色原位杂交” Shujunsha，东京，269-299（1998）

Ozawa H.,Ito T,Ochiai I,Kawata M: "Cellular localization and distribution of glucocorticoid receptor (GR) immunoreactivity and the expression of GR mRNA in the rat pituitary gland : A combined double immunohistochemistry and in situ hybridization histoche

Ozawa H.，Ito T，Ochiai I，Kawata M：“大鼠垂体中糖皮质激素受体（GR）免疫反应性的细胞定位和分布以及 GR mRNA 的表达：联合双重免疫组织化学和原位杂交组织化学

Lu H., Yuri K., Ito T., Yoshimoto K., Kawata M.: "The effects of oestrogen and progesterone on serotonin and its metabolite in lateral septum,medial preoptic area and ventromedial hypothalamic nucleus of female rats" J.Neuroendocrinology. 10. 919-926 (199

Lu H.，Yuri K.，Ito T.，Yoshimoto K.，Kawata M.：“雌激素和孕激素对雌性大鼠外侧隔、内侧视前区和腹内侧下丘脑核血清素及其代谢物的影响”J.Neuroendocrinology