ROLE OF STEROID HORMONES ON THE CELLULAR COMMUNICATION : MOLECULAR, MORPHOLOGICAL, PHYSIOLOGICAL AND BEHAVIORAL MULTIDICIPLINARY APPROACH

类固醇激素对细胞通讯的作用：分子、形态学、生理学和行为多学科方法

• 批准号: 10044314
• 负责人: KAWATA Mitsuhiro
• 金额: $ 4.22万
• 依托单位: KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044314/
• 关键词: SUBCELLULAR LOCALIZATION; STEROID HORMONE RECEPTORS; GFP; REAL-TIME IMAGING; LIGAND-DEPENDENT TRANSCRIPTION FACTOR; GLUCOCORTIOCOLDS; SEX HORMONE; NUCLEOCYTOPLASMIC TRANSLOCATION; 性ホルモン; エストロゲンレセプター; 生後発育; アポトーシス; 強制発現; 視索前野; 雌雄差; GEP

The subcellular distribution of the steroid hormone receptors has not yet been established and controversial : different methods showed different results, I.e. each type of receptor is in cytoplasmic or nuclear or both in the absence of the ligand. To elucidate the localization of receptors of adrenal steroids and estrogen, we carried out an immunocytochemistry with the use of specific antibodies and an imaging analysis by green fluorescent protein (GFP) chimera system. Without the ligand glucocorticoid receptor (GR) is in the cytoplasmic and mineralocorticoid receptor (MR) is both in the cytoplasmic and nuclear, whereas estrogen receptor (ER) a and b are both in the nucleus. Ligand induces the accumulation of all these receptors in the nucleus. The ligand-induced movement of these nuclear receptors from inactive states depends on each type of receptor.

类固醇激素受体的亚细胞分布尚未确定，也存在争议：不同的方法显示出不同的结果，即每种类型的受体在缺乏配体的情况下都位于细胞质或细胞核中，或者两者都存在。为了阐明肾上腺类固醇和雌激素受体的定位，我们利用特异性抗体和绿色荧光蛋白(GFP)嵌合体系统进行了免疫细胞化学和图像分析。在没有配体的情况下，糖皮质激素受体(GR)位于胞浆，盐皮质激素受体(MR)位于胞浆和胞核，雌激素受体(ER)a和b均位于胞核。配体诱导所有这些受体在细胞核内积聚。配体诱导的这些核受体从不活跃状态移动取决于每种类型的受体。

项目成果

Matsushita, H. et al: "Changes in serotonergic neurons in the brain of pyrithiamine-induced acute thiamine-deficient mice"Acta Neuropathol. 98. 614-621 (1999)

Matsushita, H. 等人：“吡硫胺诱导的急性硫胺素缺乏小鼠大脑中血清素能神经元的变化”Acta Neuropathol。

Hu Z., Ito T., Yuri K., Xie,C., Ozawa H., Kawata M.: "In vivo time course of morphological changes and DNA degradation during the degeneration of castration-induced apoptotic cells"Cell Tissue Res.. 294. 153-160 (1998)

Hu Z.、Ito T.、Yuri K.、Xie,C.、Ozawa H.、Kawata M.：“去势诱导凋亡细胞变性过程中形态变化和 DNA 降解的体内时间过程”细胞组织研究。

森田規之,伊藤隆男,河田光博: "脱アイソトープ実践プレトコール,カラーin situハイブリダイゼーション" 秀潤社,東京, 269-299 (1998)

Noriyuki Morita、Takao Ito、Mitsuhiro Kawata：“同位素去除实践、彩色原位杂交” Shujunsha，东京，269-299（1998）

Hu Z. et al: "Long-term lack of endogenous glucocorticoids down-regulates glucocorticoid receptor livels in the rat forebrain"Neuroscience. 90. 1185-1195 (1999)

Hu Z.等人：“长期缺乏内源性糖皮质激素会下调大鼠前脑中糖皮质激素受体的活性”神经科学。

Matsushita,H., Takeuchi,Y., Kosaka,K., Fushiki, S., Kawata,M., Sawada, T.: "Changes in serotonergic neurons in the brain of pyrithiamine-induced acute thiamine-deficient mice"Acta Neuropathol. 98. 614-621 (1999)

Matsushita，H.，Takeuchi，Y.，Kosaka，K.，Fushiki，S.，Kawata，M.，Sawada，T.：“吡硫胺诱导的急性硫胺素缺乏小鼠大脑中血清素能神经元的变化”Acta Neuropathol