ROLE OF STEROID HORMONES ON THE CELLULAR COMMUNICATION : MOLECULAR, MORPHOLOGICAL, PHYSIOLOGICAL AND BEHAVIORAL MULTIDICIPLINARY APPROACH

类固醇激素受体的亚细胞分布尚未确定且存在争议：不同的方法显示不同的结果，即每种类型的受体在没有配体的情况下存在于细胞质或核中或两者兼有。为了阐明肾上腺甾体和雌激素受体的定位，我们利用特异性抗体和绿色荧光蛋白（GFP）嵌合体系统进行了免疫细胞化学和成像分析。不含配体的糖皮质激素受体（GR）位于细胞质中，矿皮质激素受体（MR）同时位于细胞质和细胞核中，而雌激素受体（ER） a和b均位于细胞核中。配体诱导所有这些受体在细胞核中聚集。这些核受体由配体诱导的从非活性状态的运动取决于每种类型的受体。

The subcellular distribution of the steroid hormone receptors has not yet been established and controversial : different methods showed different results, I.e. each type of receptor is in cytoplasmic or nuclear or both in the absence of the ligand. To elucidate the localization of receptors of adrenal steroids and estrogen, we carried out an immunocytochemistry with the use of specific antibodies and an imaging analysis by green fluorescent protein (GFP) chimera system. Without the ligand glucocorticoid receptor (GR) is in the cytoplasmic and mineralocorticoid receptor (MR) is both in the cytoplasmic and nuclear, whereas estrogen receptor (ER) a and b are both in the nucleus. Ligand induces the accumulation of all these receptors in the nucleus. The ligand-induced movement of these nuclear receptors from inactive states depends on each type of receptor.