Electrophysiologic Response to Autonomic Receptor Stimulation of Pertussis Toxin Rreated Canimc Cardiac Muscle.

对百日咳毒素相关的犬心肌自主神经受体刺激的电生理反应。

• 批准号: 63570455
• 负责人: OANOO Mitsuru
• 金额: $ 1.28万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570455/
• 关键词: Pertussis toxin; electrophysiologic study; isoproterenol; acetylcholine; adult dog; neonatal dog; chronotropic effect; β受容体刺激; 自動能

It is proposed that abnormal autonomic control may play a role in sudden death of infants received pertussis vaccination. The biochemical effect of pertussis toxin on cardiac muscle has been shown as an increased response to catecholamines and decreased response to acetylcholine. The purpose of this study is to clarify the age related electrophysiologic effect of pertussis toxin on canine Purkinje fiber in a response to autonomic receptor stimulation.In pertussis toxin treated adult fibers isoproterenol induced positive chronotropic effect was enhanced, and acetylcholine induced negative chronotropic effect was diminished. Neonatal fiber demonstrated a less effect of isoproterenol on automatisity than adult fiber. In pertussis toxin treated neonatal fibers isoproterenol induced positive inotropic effect was not enhanced.Beta adrenergic stimulation of neonatal Purkinje fiber by isoproterenol which is not absorbed by the nerve ending, has a less effect on automaticity. This may partly due to an age related difference of GTP binding protein or beta adrenergic receptor.

提示百日咳疫苗接种后婴儿猝死可能与植物神经控制异常有关。百日咳毒素对心肌的生化作用表现为对儿茶酚胺的反应增加和对乙酰胆碱的反应降低。本研究的目的是阐明百日咳毒素对犬浦肯野纤维在自主神经感受器刺激反应中的电生理效应，百日咳毒素处理的成年纤维中异丙肾上腺素引起的正性变时效应增强，乙酰胆碱引起的负性变时效应减弱。异丙肾上腺素对新生纤维自律性的影响小于成年纤维。在百日咳毒素处理的新生纤维中，异丙肾上腺素诱导的正性变力作用没有增强。异丙肾上腺素对新生浦肯野纤维的β肾上腺素能刺激不被神经末梢吸收，对自律性的影响较小。这可能部分是由于年龄相关的GTP结合蛋白或β肾上腺素能受体的差异。