Histologic and Electrophysiologic Study of Normal Neural Retinal Cell Transplantation in Mice with Retinal Degeneration

视网膜变性小鼠正常神经视网膜细胞移植的组织学和电生理学研究

• 批准号: 11671761
• 负责人: YAMAMOTO Shuichi
• 金额: $ 2.11万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671761/
• 关键词: Retinal dystrophic mice; Neural retinal cell transplantation; 網膜変性; 網膜移植; 網膜電図

1) The normal neural retinas obtained from transgenic mice have been successfully transplanted into the subretinal space of retinal dystrophic mice without immune suppression. Any sign of the immune rejection was not observed by the fundoscopic examination.2) It was found that the orientation of the differentiating photoreceptor outer segments were varied in each experiment and that the proper orientation could hardly archived in our current method.3) Several attempts to improve the results have been performed, and further histological and electrophysiological studies are needed.4) The TGF-beta neutralizing antibody successfully suppresses the development of experimental proliferative vitreoretinopathy in rabbit eyes.5) Electroretinograms were examined in patients with various retinal diseases to study retinal function, in order to compare with those in experimental animals.6) An LED built-in electrode was developed and tested in human eyes.

1)从转基因小鼠获得的正常神经视网膜已成功地移植到视网膜营养不良小鼠的视网膜下腔中，而不需要免疫抑制。眼底镜检查没有观察到任何免疫排斥的迹象。2）发现在每个实验中分化的光感受器外节的取向是不同的，并且在我们目前的方法中很难获得正确的取向。3）已经进行了几次尝试来改善结果，需要进一步的组织学和电生理学研究。4）TGF-β中和抗体成功地抑制了兔眼实验性增殖性玻璃体视网膜病变的发展。5）在患有各种视网膜疾病的患者中检查视网膜电图以研究视网膜功能，以便与实验动物中的视网膜电图进行比较。6）开发LED内置电极并在人眼中进行测试。

项目成果

Mitamura Y: "Macrophage migration inhibitory factor levels in the vitreous of patients with proliferative vitreoretinopathy"American Journal of Ophthalmology. 128. 793-765 (1999)

Mitamura Y：“增殖性玻璃体视网膜病变患者玻璃体中巨噬细胞迁移抑制因子的水平”美国眼科杂志。

Mitamura Y: "Macrophage migration inhibitory factor level in the vitreous"American Journal of Ophthalmology. 129. 678-680 (2000)

Mitamura Y：“玻璃体内巨噬细胞迁移抑制因子水平”美国眼科杂志。

Yamamoto,S.et al.: "Electroretinograms and visuall evoked potentials alicsted by spectral stimuli in a patient with enhauced S-cone syndrome"Jpn J Ophthalmol. 43. 433-437 (1999)

Yamamoto,S.et al.：“患有增强型 S 锥综合征的患者通过光谱刺激进行的视网膜电图和视觉诱发电位”Jpn J Ophamol。

Yamamoto T: "Expression and possible role of Activin A in proliferative vitreoretinal diseases"Japanese Journal of Ophthalmology. 44. 221-226 (2000)

Yamamoto T：“激活素 A 在增殖性玻璃体视网膜疾病中的表达和可能的作用”日本眼科杂志。

Yamamoto S: "Morphological and functional analyses of diabetic macular edema by optical coherence tomography and multifocal electroretinograms"Graefe's Archive for Clinical and Experimental Ophthalmology. (印刷中).

Yamamoto S：“通过光学相干断层扫描和多焦视网膜电图对糖尿病黄斑水肿进行形态学和功能分析”格雷夫临床和实验眼科档案（正在出版）。