Transmembrane Signaling System in Psoriatic Keratinocytes.

银屑病角质形成细胞的跨膜信号系统。

• 批准号: 63570464
• 负责人: IIZUKA Hajime
• 金额: $ 1.41万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570464/
• 关键词: Psoriasis; Adenylate cyclase; G protein; Protein kinase C; Calcium; Phorbol ester; Phospholipase C; プロテインキナ-ゼC; アデニル酸ミクラ-ゼ; 癌遺伝子; ras; 尋常性乾癬; アデニル酸シクラーゼ; G蛋白質(guanine nucleotidl binding protein); プロテインキナーゼC; tumor promoter; TPA(tetradecana

Recent evidence suggests that the breakdown of inositol phospholipids is an important transmembrane signalling system that is composed of two kinds of signals : the diacylglycerol (DAG) -protein kinase C signal, and the inositol trisphosphate - Ca^<++> signal. The system is activated in psoriatic hyperproliferative keratinocytes, which might be associated with the altered function of other transmembrane signaling systems such as the adenylate cyclase. The epidermal adenylate cyclase was shown to be modulated by various protein kinase C activators, which include tumor promoting phorbol esters. The modulation was similar to that of psoriatic involved epidermis. Despite the finding that the classical protein kinase C isozymes are activated by DAG and also by Ca^<++> at higher concentrations, the epidermal adenylate cyclase responses were modulated only by DAG-protein kinase C signal, but not by Ca^<++> signal. Our results indicate that the epidermal adenylate cyclase system is modulated by protein kinase C, which might be the novel Ca^<++>insensitive form of protein kinase C recently described by Kuroki and C -workers.

最近的证据表明，肌醇磷脂的降解是一个重要的跨膜信号系统，它由两种信号组成：二酰甘油(DAG)-蛋白激酶C信号和肌醇三磷酸钙++信号。该系统在银屑病过度增殖的角质形成细胞中被激活，这可能与其他跨膜信号系统的功能改变有关，如腺苷环化酶。研究表明，表皮腺苷环化酶受多种蛋白激酶C激活剂的调节，其中包括促进肿瘤的佛波酯。这种调节类似于银屑病受累的表皮。尽管发现经典的蛋白激酶C同工酶可被DAG激活，但在较高浓度下也可被Ca^&lt；++&gt；激活，但表皮的腺苷环化反应仅受DAG-蛋白激酶C信号的调节，而不受Ca^&lt；++&gt；信号的调节。我们的结果表明，表皮腺苷环化酶系统受蛋白激酶C的调节，这可能是黑木和C-Worker最近描述的一种新的钙不敏感的蛋白激酶C形式。

项目成果

Hidetoshi Takahashi: "Adenylate cyclase system in fetal rat keratinizing epidermal cells (FRSK cells) and SV40ーtransformed human keratinocytes" Journal of Dermatology. 17. 457-464 (1990)

Hidetoshi Takahashi：“胎鼠角化表皮细胞（FRSK 细胞）和 SV40 转化的人角质形成细胞中的腺苷酸环化酶系统”《皮肤病学杂志》17. 457-464 (1990)。

Hajime Iizuka: "Effects of the tumor promoter,phorbol 12 myristate 13 acetate,on the epidermal adenylate cyclase.suptem" Journal of Investigative Dermatology. 93. 387-391 (1989)

Hajime Iizuka：“肿瘤促进剂佛波醇 12 肉豆蔻酸 13 乙酸酯对表皮腺苷酸环化酶的影响。suptem”《皮肤病学研究杂志》。

Hidetoshi Takahashi,Hajime Iizuka: "No evidence for the mutation of ras gene in psoriatic epidermis." Archives of Dermatological Research.

Hidetoshi Takahashi、Hajime Iizuka：“没有证据表明银屑病表皮中 ras 基因发生突变。”

Hidetoshi, Takahashi et al: "Adenylate cyclase system in feral rat keratinizing epidermal cells) and SV40-transformed human keratinocytes." J Dermatol. 17. 457-464 (1990)

Hidetoshi、Takahashi 等人：“野鼠角质化表皮细胞中的腺苷酸环化酶系统和 SV40 转化的人角质形成细胞。”

Hidetoshi Takahashi: "No evidence for the mutation of ras gene in psoriatic epidermis" Archives of Dermatological Research. 282. 8-11 (1990)

Hidetoshi Takahashi：“没有证据表明银屑病表皮中 ras 基因发生突变”皮肤病学研究档案。