Kinetic propertie of three Darier disease SERCA26 mutants.

三种 Darier 病 SERCA26 突变体的动力学特性。

• 批准号: 14370256
• 负责人: IIZUKA Hajime
• 金额: $ 5.44万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370256/
• 关键词: Darier disease; endoplasmic reticulum; calcium pump; keratinization

The functional abnormalities in Darier disease-causing calcium ATPase (Serca2B) mutants were explored. Among 51mutants, 15 showed negligible expression of SERCA2b, 21 showed negative ATPase activities, and 8 showed functional uncoupling of ATPase and calcium pump activities in Cos1 cells. The remaining 3 showed unique characteristics. They exhibited nearly normal expression, high ATPase and coupled calcium transport activities that were essentially identical (L321F) or slightly lower (I274V and M719I) as compared with those of the wild type. M719I and L321F possess 2-3 fold reduced affinity for cytoplasmic calcium, whereas I274V possesses the normal high affinity. L321F exhibited also the remarkably reduced sensitivity to the feedback inhibition of the transport cycle by accumulated luminal calcium. Thus different abnormalities in Darier disease causing ATPase (SERCA2b) showed distinct types of altered calcium homeostasis in Cos1 cells.

探讨了Darier病致病钙ATPase(Serca2B)突变体的功能异常。在51个突变株中，15个突变株的SERCA2b表达可忽略不计，21个突变株的ATPase活性为阴性，8个突变株的ATPase和钙泵活性在功能上解偶联。其余3例表现出独特的特征。它们表现出几乎正常的表达，高ATPase和偶联钙转运活性，与野生型基本相同(L321F)或略低(I274V和M719I)。M719I和L321F对胞浆钙的亲和力降低2-3倍，而I274V具有正常的高亲和力。L321F对鲁米诺钙蓄积对转运周期的反馈抑制的敏感性也显著降低。因此，引起ATPase(SERCA2b)的Darier病的不同异常在Cos1细胞中显示出不同类型的钙稳态改变。

项目成果

Distinct types of abnormality in kinetic properties of three Darier disease-causing sarco(endo)plasmic reticulum Ca2+-ATPase mutants that exhibit normal expression and high Ca2+ transport activity

• DOI: 10.1074/jbc.m404887200
• 发表时间: 2004-08-20
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Sato, K;Yamasaki, K;Suzuki, H
• 通讯作者: Suzuki, H

Prediction of a coding sequence for a novel type II keratin

新型 II 型角蛋白编码序列的预测

• 发表时间: 2005
• 期刊: Journal of Dermatological Science 37
• 作者: Senshu T;Iizuka H;et al.
• 通讯作者: et al.

Cyclic AMP differentially regulates cell proliferation of normal human keratinocytes through ERK activation depending on the expression pattern of B-Raf

• DOI: 10.1007/s00403-004-0478-z
• 发表时间: 2004-04-01
• 期刊: ARCHIVES OF DERMATOLOGICAL RESEARCH
• 影响因子: 3
• 作者: Takahashi, H;Honma, M;Iizuka, H
• 通讯作者: Iizuka, H

Epidermal lamellar granules transport different cargoes

表皮层状颗粒运输不同的货物

• 发表时间: 2005
• 期刊: Journal of Investigative Dermatology 122
• 作者: Ishida-Yamamoto A;Iizuka H;et al.
• 通讯作者: et al.

Unique keratinization process in psoriasts : late differentiation markers are abolished

银屑病患者独特的角化过程：晚期分化标记被废除

• 发表时间: 2004
• 期刊: Journal of Dermatology 31
• 作者: Iizuka H;Takahashi H;Honma M;Ishida-Yamamoto A
• 通讯作者: Ishida-Yamamoto A