Transmembrane Signalling System in Psoriasis

1)银屑病皮损表皮以β-肾上腺素能腺苷环化酶反应缺陷为特征。2)糖皮质激素通过诱导β2-肾上腺素能受体来增强角质形成细胞的β-肾上腺素能腺苷环化酶反应。3)蛋白激酶C激活剂诱导角质形成细胞角化包膜的主要成分之一--总蛋白的表达。4)维甲酸Ro 10-1670能增强猪皮肤器官培养体系中β-肾上腺素能腺苷环化酶的反应。然而，在FRSK培养体系中，维甲酸降低了β-肾上腺素能腺苷环化酶的反应。在后者系统中，伴随着FRSK细胞胸腺嘧啶核苷掺入增加的β-肾上腺素能反应减弱。5)银屑病皮损表皮超氧化物歧化酶活性显着降低。这是由于铜、锌-超氧化物歧化酶活性降低所致。免疫组织化学染色显示，与真皮乳头相对应的基底细胞层中可见铜锌超氧化物歧化酶染色强度降低。

1) Psoriatic involved epidermis was characterized by defective beta-adrenergic adenylate cyclase response. This was observed by the treatment of keratinocytes with protein kinase C activators as well as by the treatment of cells with the beta-adrenergic agonists.2) Glucocorticoids augment the beta-adrenergic adenylate cyclase response of keratinocytes through the induction of the beta2-adrenergic receptor.3) Protein kinase C activators induced the expression of involucrin, that is one of the major constituent of keratinocyte cornified envelope. The induction of involucrin by the protein kinase C activators was jun/fos-dependent process and was inhibited by glucocorticoids.4) Retinoid, Ro 10-1670, augmented the beta-adrenergic adenylate cyclase response in pig skin organ culture system. However, the retinoid decreased the beta-adrenergic adenylate cyclase response in FRSK culture system. In the latter system the decreased beta-adrenergic response was accompanied by the increased thymidine incorporation of FRSK cells.5) Superoxide dismutase activity was markedly decreased in the psoriatic involved epidermis. This was due to the decreased Cu, Zn-SOD activity. Immunohistochemical staining showed that the decreased Cu, Zn-SOD staining pattern was observed in the basal cell layr corresponding to the dermal papillae.