Possible effect of splenectomy on liver regeneration in cirrhotic rats.

本实验方案旨在确定脾切除术对肝硬化大鼠肝脏再生的可能影响。首先，研究肝切除术后的病理生理，关注细胞动力学、膜流动性和膜功能的变化。正常大鼠肝切除70%后6小时膜流动性增加，24小时后达到峰值。与此相反，用碘化丙啶和BrdU抗体进行2色分析的DNA直方图显示，s期核在肝切除术后24和48小时达到峰值。为了产生肝硬化，腹腔注射4%硫代乙酰胺10周。随着肝硬化的发展，膜流动性下降，细胞动力学分析显示2C室的比例较正常人增加。肝硬化大鼠肝切除术70%后，约三分之二的肝硬化大鼠在48小时内死亡。然而，脾切除术并没有提高生存率。然后行50%肝切除术以提高生存率。通过。肝部分切除后，半数正常大鼠肝膜流动性增加，出现s期核。然而，脾切除术并没有显著改变膜流动性或细胞动力学。以上结果表明，即使在肝部分切除术后的肝硬化也会发生再生过程，脾切除术对肝部分切除术后膜流动性或再生的变化没有显著影响。

The present experimental protocol is to identify a possible effect of splenectomy on liver regeneration in cirrhotic rats. First, the pathophysiology along with hepatic resection was studied, paying an attention at changes in cell kinetics, membrane fluidity as membraneous function. In normal rats, membrane fluidity was increased at 6 hours after 70% hepatectomy, making a peak at 24 hours later. By contrast, DNA histogram using 2 color analysis with propidium iodide and BrdU antibody showed a peak of S-phased nuclei at 24 and 48 hours after hepatectomy. In order to produce eirrhosis, an intraperitoneal injection of 4% thioacetoamide was performed for 10 weeks. Along with development of cirrhosis, membrane fluidity decreased and the analysis of cell kinetics showed an increasing proportion of 2C compartment compared to that of normal. By 70% hepatectomy in the cirrhotic rats, about two of third cirrhotic rats died within 48 hours later. However, splenectomy didnot improve the survival rate. Then, 50% hepatectomy was undergone to improve survival rate. By. this, membrane fluidity was increased and also, S-phased nuclei appeared in half of proportion of normal rats after partial hepatectomy. However, splenectomy did not alter significantly membrane fluidity or cell kinetics. Above results suggest that regeneration process occurs even in cirrhotic liver after partial hepatectomy and splenectomy does not affect significantly the changes in membrane fluidity or regeneration following partial hepatectomy.