Transient receptor potential A1 mediates gastric distention-induced visceral pain in rats

TRPA 1主要与感觉神经肽一起在背根神经节（DRG）和结状神经节（NG）神经元以及大鼠胃的神经纤维中表达。鞘内注射TRPA 1反义核酸可减弱内脏反应，并抑制DRG神经元ERK 1/2的激活，但对胃扩张（GD）引起的NG神经元无影响。此外，鞘内和腹膜内注射TRPA 1抑制剂HC-03003抑制了对毒性GD的反应。伤害性GD激活背根节神经元中的TRPA 1可能参与急性内脏痛。

TRPA1 was predominantly expressed with sensory neuropeptides in dorsal root ganglion(DRG) and nodose ganglion(NG) neurons, and in nerve fibers in the rat stomach. Intrathecal injection of TRPA1 antisense attenuated the visceromotor response, and suppressed ERK1/2 activation in the DRG, but not NG, neurons produced by gastric distention(GD). Furthermore, intrathecal and intraperitoneal injections of the TRPA1 inhibitor HC-03003 suppressed the response to noxious GD. The activation of TRPA1 in DRG neurons by noxious GD may be involved in acute visceral pain.