Transient receptor potential A1 mediates gastric distention-induced visceral pain in rats

TRPA1主要表达于背根神经节(DRG)和结状神经节(NG)神经元以及大鼠胃的神经纤维中。鞘内注射TRPA1反义寡核苷酸可减弱胃扩张引起的内脏运动反应，并抑制DRG神经元ERK1/2的激活，但不抑制NG神经元的激活。此外，鞘内和腹膜内注射TRPA1抑制剂HC-03003可抑制对伤害性GD的反应。伤害性GD激活DRG神经元TRPA1可能参与了急性内脏痛的发生。

TRPA1 was predominantly expressed with sensory neuropeptides in dorsal root ganglion(DRG) and nodose ganglion(NG) neurons, and in nerve fibers in the rat stomach. Intrathecal injection of TRPA1 antisense attenuated the visceromotor response, and suppressed ERK1/2 activation in the DRG, but not NG, neurons produced by gastric distention(GD). Furthermore, intrathecal and intraperitoneal injections of the TRPA1 inhibitor HC-03003 suppressed the response to noxious GD. The activation of TRPA1 in DRG neurons by noxious GD may be involved in acute visceral pain.