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Fetal surgical prevention of genetic limb anomalies in mice.

胎儿手术预防小鼠遗传性肢体异常。

基本信息

批准号：
63570715
负责人：
NARUSE Ichiro
金额：
$ 1.22万
依托单位：
Institute for Developmental Research, Aichi Prefectural Colony
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570715/
关键词：
fetal surgery laser limb anomaly exo utero fetal therapy mouse genetic anomaly 胎仔外科胎仔手術レーザー四肢奇形

项目摘要

The aim of this project is the promotion of fetal therapy in congenital anomalies by means of fetal surgery. The development of ultrasonography has enabled the perinatal team to diagnose antenatally a wide variety of congenital anomalies. Recently, investigators have reported the in utero placement of catheters in fetuses with bladder obstruction and hydrocephalus in an attempt to prevent further organ damage resulting from tissue compression. In the present study, fetal laser surgery to prevent congenital anomalies was used in mouse embryos exo utero.The homozygous Polydactyly Nagoya (Pdn/Pdn) mice exhibit 1-3 extra digits both in the fore- and hindlimbs preaxialy. The extra digital rays in the left hindlimbs of Pdn/Pdn fetuses were irradiated with 2 watts argon laser beam for 0.3 sec. on day 13 of gestation exo utero.The left hindlimbs of the Pdn/Pdn fetuses received fetal laser surgery lost their preaxial extra digits on day 18 of gestation, resulting in 5 digits, though their first digit was triphalangia.We don't consider now that fetal surgery of polydactyly in the human fetus is much superior to the cutting of the extra digits after birth. However, the combination of laser instrumentation with the fetoscope and/or with the real-time ultrasound scanner will promote the study of preventing congenital anomalies such as not only the polydactyly and syndactyly, but also teratoma and parasitic twin in the human.

该项目的目的是通过胎儿手术促进先天性畸形的胎儿治疗。超声检查的发展使围产期小组能够在产前诊断各种各样的先天性异常。最近，研究者报道了在患有膀胱梗阻和脑积水的胎儿中放置导管以试图防止组织压迫导致的进一步器官损伤。在本研究中，胎儿激光手术，以防止先天性异常的小鼠胚胎子宫外，纯合子多趾名古屋（Pdn/Pdn）小鼠表现出1-3额外的数字在前和后肢轴前。用2 W氩激光照射Pdn/Pdn胎儿左后肢的额外趾线0.3秒。Pdn/Pdn胎儿行胎儿激光手术，于孕18天左后肢失去轴前多指，第一指为三趾，但仍有5指，目前认为胎儿多指手术并不比出生后切除多指优越上级多少。然而，激光仪器与胎儿镜和/或与实时超声扫描仪的组合将促进预防先天性异常的研究，例如不仅是多指畸形和并指畸形，而且是畸胎瘤和寄生双胞胎。