Arhinencephaly induced by fetal laser surgery exo utero in mice.

在本研究中，有人建议，非附件的嗅神经的端脑阻断诱导的嗅球从端脑的遗传archinencephalic小鼠胚胎（Pdn/Pdn）。结果表明，胚胎期激光手术对嗅球原基的破坏是导致胼胝体发育不全和前连合连接不规则的触发因素，推测嗅神经与端脑的不连接可能影响了嗅神经中促黄体生成素释放激素（LHRH）神经元的迁移。结果表明，嗅球原基中的前体僧帽细胞凋亡是由于嗅神经元的无神经支配所致，梨状皮质中的前体神经元凋亡是由于Pdn/Pdn脑中僧帽细胞死亡导致的无神经支配所致。在胚胎激光手术破坏嗅球原基的胚胎中，梨状皮质前体神经元也出现凋亡，提示Pdn/Pdn脑和胚胎激光手术诱导的无嗅脑中，前体神经元的凋亡和脑异常的表现是连续的。

In the present study, it has been suggested that non-attachment of the olfactory nerve to the telencephalon blocked the induction of the olfactory bulbs from the telencephalon in genetic archinencephalic mouse embryos (Pdn/Pdn). And it was shown that the destruction of the olfactory bulb anlage using fetal laser surgery exo utero becomes a trigger of agenesis of the corpus callosum and irregular connection of the anterior commissure in later morphogenesis of the mouse brain.We speculated that the migration of luteinizing hormone-releasing hormone (LHRH) neurons might be disturbed in the olfactoy nerve because of the non-attachment of the olfactory nerve to the telencephalon. partial arrest of LHRH neurons was observed.It was suggested that apoptosis of the precursor mitral cells in the anlage of the olfactory bulb was induced by non-innervation of olfactory neurons, and apoptosis of the precurosor neurons in the pyriform cortex was induced by the non-innervation caused by the death of mitral cells in Pdn/Pdn brain. Apoptosis in the precursor neurons in the pyriform cortex was also observed in the embryos whose anlages of the olfactory bulbs were destructed by fetal laser surgery.Thus, sequential apoptosis of the precursor neurons and sequential manifestation of the brain abnormalities were proposed in Pdn/Pdn brain and also in the arhinencephalic brains induced experimentally by fetal laser surgery exo utero.