Serological diagnosis of antisalivary duct antibody and antinuclear antibody by human salivary duct cells in Sjogren's syndrome

人唾液管细胞抗唾液管抗体和抗核抗体对干燥综合征的血清学诊断

• 批准号: 63570926
• 负责人: SAKAMOTO Maya
• 金额: $ 1.34万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570926/
• 关键词: Antisalivary duct antibody; Antinuclear antibody; Human salivary duct cells; Sjogren's syndrome; シェーグレン症候群

This study was designed to evaluate the serological diagnosis of Antisalivary duct antibody(ASDA) and Antinuclear antibody(ANA)in sera of patients with Sjbgren's syndrome(SjS) using Human salivary duct(HSD) cells transformed by SV40 in vitro.The detection of ASDA and ANA in sera from patients with SiS were examined by indirect immunofluorescent technique using HSD cells as substrate and the results were summarized as follows;1.ASDA could be detected in 78% of sera from patients with definite SiS, but not in sera of patients with probable SjS and control patients. 2.Correlation between ASDA positive and lymphocytic sialoadenitis of definite SjS patients was recognized clearly. 3.ANA was found in 87% of sera from patients with definite SjS and 5 % of sera from patients with probable SjS. 4.Three staining types of speckled, homogeneous and peripheral patterns could be classified in FITC positive nuclear. 5.The speckled pattern of antinuclear antibody is frequent findings in sera from patients who had clinical features compatible with SjS.These results indicated that HSD cells were very useful for serological diagnosis of SjS.

应用SV40体外转化的人唾液管(HSD)细胞对干燥综合征(Sjbgren‘s综合征)患者血清中的抗涎管抗体(Asda)和抗核抗体(ANA)进行血清学诊断。以SIS患者血清为底物，采用间接免疫荧光技术检测SIS患者血清中Asda和ANA。2.明确了SjS患者Asda阳性与淋巴细胞性涎腺炎的相关性。3.确诊SjS患者血清ANA阳性率为87%，疑似SjS患者血清ANA含量为5%。4.FITC阳性细胞核可分为斑点型、均质型和周边型三种染色类型。5.抗核抗体斑点模式是临床表现与SjS相容的患者中常见的表现，提示HSD细胞对SjS的血清学诊断有重要价值。

项目成果

Atsuko Osawa: "Immunologic study of Sjogrens syndrome No.2 Establishment of human salivary duct cells transformed by SV40" J.Jpn.Stomatol.Soc.37(4):879-885, 1988.

Atsuko Osawa：“干燥综合征的免疫学研究 No.2 SV40 转化的人唾液管细胞的建立”J.Jpn.Stomatol.Soc.37(4)：879-885，1988。

阪本真弥: 日本口腔科学会雑誌. 38. 172-186 (1989)

Shinya Sakamoto：日本口腔医学会杂志 38. 172-186 (1989)。

Maya Sakamoto: "Serological diagnosis of antisalivary duct antibody(ASDA) and antinuclear antibody(ANA) by human salivary duct(HSD) cells in Sjogrens syndrome(SjS)" J.Jpn.Stomatol.Soc.38(1):172-186, 1989.

Maya Sakamoto：“干燥综合征 (SjS) 中人唾液管 (HSD) 细胞抗唾液管抗体 (ASDA) 和抗核抗体 (ANA) 的血清学诊断” J.Jpn.Stomatol.Soc.38(1):172-186

大澤篤子: 日本口腔科学会雑誌. 37. 879-885 (1988)

大泽敦子：日本口腔医学会杂志 37. 879-885 (1988)。

阪本真弥: "ヒト唾液腺導管樹立細胞によるSjogren症候群の血清学的診断-抗唾液腺管抗体と抗核抗体-" 日本口腔科学会雑誌. 38. 172-186 (1989)

Shinya Sakamoto：“使用人唾液腺管建立细胞的干燥综合征的血清学诊断 - 抗唾液管抗体和抗核抗体 -” 日本口腔医学会杂志 38. 172-186 (1989)。