Serological diagnosis of antisalivary duct antibody and antinuclear antibody by human salivary duct cells in Sjogren's syndrome

本研究应用SV40体外转化的人涎腺导管（HSD）细胞，以间接免疫荧光技术检测Sjbgren综合征（SjS）患者血清中抗涎腺导管抗体（ASDA）和抗核抗体（ANA），结果如下：1.Sis患者血清中ASDA的检出率为78%，而可疑SjS患者和正常对照组血清中ASDA的检出率均为100%。2.明确了ASDA阳性与淋巴细胞性涎腺炎的相关性。3. ANA在确诊的SjS患者血清中的检出率为87%，在疑似SjS患者血清中的检出率为5%。4. FITC阳性细胞核可分为斑点型、均匀型和周边型三种染色类型。5.抗核抗体斑点样模式在符合SjS临床特征的患者血清中常见，提示HSD细胞对SjS的血清学诊断有重要意义。

This study was designed to evaluate the serological diagnosis of Antisalivary duct antibody(ASDA) and Antinuclear antibody(ANA)in sera of patients with Sjbgren's syndrome(SjS) using Human salivary duct(HSD) cells transformed by SV40 in vitro.The detection of ASDA and ANA in sera from patients with SiS were examined by indirect immunofluorescent technique using HSD cells as substrate and the results were summarized as follows;1.ASDA could be detected in 78% of sera from patients with definite SiS, but not in sera of patients with probable SjS and control patients. 2.Correlation between ASDA positive and lymphocytic sialoadenitis of definite SjS patients was recognized clearly. 3.ANA was found in 87% of sera from patients with definite SjS and 5 % of sera from patients with probable SjS. 4.Three staining types of speckled, homogeneous and peripheral patterns could be classified in FITC positive nuclear. 5.The speckled pattern of antinuclear antibody is frequent findings in sera from patients who had clinical features compatible with SjS.These results indicated that HSD cells were very useful for serological diagnosis of SjS.