NMR studies of membrane proteins and peptides in novel amphiphilic mesophases

新型两亲中间相膜蛋白和肽的核磁共振研究

• 批准号: DP0450808
• 负责人: Em/Prof Philip Kuchel
• 金额: $ 88.38万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-02-01 至 2009-01-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0450808
• 关键词: NMR; studies; membrane; proteins; peptides

Membrane proteins are the next frontier in structural biology. Our goal is the structural and mechanistic characterization of the proteins and peptides from platypus venom and a cardiac potassium ion channel, HERG, that has a particular role in the suppression of cardiac arrhythmias. To do this we will refine and develop methods using amphiphilic mesophases and micelles and state-of-the-art NMR spectroscopy. Electrophysiological analysis of ion channels and interactions with toxins will relate NMR structures to function. The NMR methodologies we develop will have broad applicability to membrane proteins in general.

膜蛋白是结构生物学的下一个前沿。我们的目标是从鸭嘴兽毒液和心脏钾离子通道，HERG，具有抑制心律失常的特殊作用的蛋白质和肽的结构和机械特性。为此，我们将使用两亲性中间相和胶束以及最先进的NMR光谱来改进和开发方法。离子通道的电生理分析和与毒素的相互作用将NMR结构与功能联系起来。我们开发的NMR方法将具有广泛的适用性，一般膜蛋白。